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Methods portfolio

IFN-γ ELISpot

ELISpot

ELISpot
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With the help of an ELISpot assay (Enzyme Linked Immuno Spot Assay), individual reactive T cells can be functionally detected in a PBMC sample. The method is ideal for screening and for monitoring of successful vaccination.

Fluorescence-activated cell scanning (FACS)

FACS

FACS
© dkfz.de

The phenotypic composition of a sample can be determined by fluorescence-activated cell scanning (FACS) measurement. Within a sample a variety of cellular markers can be used.

Luminex

Luminex

Luminex
© dkfz.de

A luminex measurement offers the possibility to determine 27 cytokines / chemokines simultaneously within a sample and measurement. As a result, cytokine profiles and chemokine profiles can be analyzed.

ELISA

ELISA

© dkfz.de

An ELISA (Enzyme-linked Immunosorbent Assay) can be used for a wide variety of approaches. In our laboratory we use the ELISA to detect specific antibody reactions in the blood serum and to determine relative antibody titers.

TCR-sequencing

Each T cell expresses only one T cell receptor (TCR) clone. With the help of TCR sequencing, the TCR repertoire at the time of blood sampling can be analyzed and, if necessary, follow it over time. It is possible to detect a clonal expansion of antigen-specific T cells.

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