Research
- Research Topics
- Cell Biology and Tumor Biology
- Stem Cells and Cancer
- Experimental Hematology
- Molecular Embryology
- Signal Transduction and Growth Control
- Epigenetics
- Redox Regulation
- Vascular Oncology and Metastasis
- Clinical Neurobiology
- Molecular Neurogenetics
- Chaperones and Proteases
- Vascular Signaling and Cancer
- Molecular Neurobiology
- Molecular Biology of Centrosomes and Cilia
- Dermato-Oncology
- Molecular Hematology - Oncology
- Stress-induced Activation of Hematopoietic Stem Cells
- Metastatic Niches
- Molecular Leukemogenesis
- DNA Repair Mechanisms and Cancer
- Neuronal Signaling and Morphogenesis
- Cell Signaling and Metabolism
- Cell Fate Engineering and Disease Modeling
- Cancer Drug Development
- Metabolism and Stem Cell Plasticity
- Team
- Open positions
- Associated unit
- Cell Morphogenesis and Signal Transduction
- Helmholtz Professorship Cell Biology
- Functional and Structural Genomics
- Biophysics of Macromolecules
- Cancer Epigenomics
- Molecular Genome Analysis
- Applied Functional Genomics
- Molecular Genetics
- Pediatric Neurooncology
- Applied Bioinformatics
- Cancer Genome Research
- Chromatin Networks
- Functional Genome Analysis
- Theoretical Systems Biology
- Neuroblastoma Genomics
- Signaling and Functional Genomics
- Signal Transduction in Cancer and Metabolism
- Brain Cancer Metabolism
- Neurooncology
- Pediatric Oncology
- RNA Biology and Cancer
- Systems Biology of Signal Transduction
- Neuropathology
- Molecular thoracic Oncology
- Proteomics of Stem Cells and Cancer
- Computational Genomics and System Genetics
- Regulatory Genomics and Cancer Evolution
- Pediatric Glioma Research
- Somatic Evolution and Early Detection
- Bioinformatics and Omics Data Analytics
- Translational Control and Metabolism
- Mechanisms of Leukemogenesis
- Brain Tumor Translational Targets
- Molecular Genetics of Breast Cancer
- Functional Proteome Analysis
- Translational Functional Cancer Genomics
- Cancer Risk Factors and Prevention
- Tumor Immunology
- Imaging and Radiooncology
- Radiology
- Research
- Contrast Agents In Radiology Research Group
- Neuro-Oncologic Imaging Research Group
- Radiological Early Response Assessment Of Modern Cancer Therapies
- Imaging In Monoclonal Plasma Cell Disorders
- 7 Tesla MRI - Novel Imaging Biomarkers
- Functional Imaging
- Visualization And Forensic Imaging
- PET/MRI
- Dual- and Multienergy CT
- Radiomics Research Group
- Prostate Research Group
- Breast Imaging Research Group
- Bone marrow
- Musculoskeletal Imaging
- Staff
- Patients
- Research
- Medical Physics in Radiology
- X-Ray Imaging and Computed Tomography
- Medical Informatics for Translational Oncology
- Radiopharmaceutical Chemistry
- Medical Physics in Radiation Oncology
- Faculty & Staff
- DKFZ PhD Programm
- Research
- Computational Patient Models
- Applied Medical Radiation Physics
- Therapy Planning - Development
- Current Projects
- Former Projects
- Adaptive Radiotherapy
- Registration
- Functional Imaging
- Knowledge based radiotherapy planning (TAPIR)
- New technologies for transferring therapeutical knowledge (IRIS)
- Planning System for proton therapy of eye tumors (OCTOPUS)
- Image Processing and Represenation (VIS-A-VIS)
- Stereotactic laser neurosurgery (STELA)
- Contact
- Research Interest
- Radiotherapy Optimization
- Medical Engineering
- Novel Detection Techniques for Ion Beams
- Physical Quality Assurance in Radiation Therapy
- Ion Beam Therapy
- MR-guided RadioTherapy
- Clinical Research Group
- Publications
- Vacancies
- Awards
- Education and Training
- Events
- Biomedical Physics in Radiation Oncology
- Computer Assisted Medical Interventions
- Medical Image Computing
- Optical Nanoscopy
- Radiooncology - Radiobiology
- Radiation Oncology
- Molecular Radiooncology
- Nuclear Medicine
- Medical Imaging and Radiology - Cancer Prevention
- Translational Radiation Oncology
- Molecular Mechanisms of Head and Neck Tumors
- Radiology
- Infection, Inflammation and Cancer
- Tumor Virology
- Viral Transformation Mechanisms
- Pathogenesis of Virus-Associated Tumors
- Applied Tumor Biology
- Virus-associated Carcinogenesis
- Chronic Inflammation and Cancer
- Episomal-Persistent DNA in Cancer- and Chronic Diseases
- Immunotherapy and Immunoprevention
- Cell Plasticity and Epigenetic Remodeling
- Cellular Polarity and Viral Infection
- Noroviruses
- Laboratory of Oncolytic Virus Immuno-Therapeutics
- Infections and Cancer Epidemiology
- Tumorvirus-specific Vaccination Strategies
- Mammalian Cell Cycle Control Mechanisms
- Molecular Therapy of Virus-Associated Cancers
- DNA Vectors
- Other Units
- Cell Biology and Tumor Biology
- Research Groups A-Z
- Junior Research Groups
- Core Facilities
- Center for Preclinical Research
- Chemical Biology Core Facility
- Genomics and Proteomics
- Imaging and Cytometry
- Information Technology
- Library
- Kataloge - Catalogues
- Zeitschriften - Journals
- E-Books - Ebooks
- Datenbanken - Databases
- Dokument-Lieferung - Document Delivery
- Publikationsdatenbank - Publication database
- DKFZ Archiv - DKFZ Archive
- Open Access
- Science 2.0
- Ansprechpartner - Contact
- More Information - Service
- Anschrift - Address
- Antiquariat - Second Hand
- Aufstellungssystematik - Shelf Classification
- Ausleihe - Circulation
- Benutzerhinweise - Library Use
- Beschaffungsvorschläge - Desiderata
- Fakten und Zahlen - Facts and Numbers
- Kooperationen, Konsortien - Cooperations, Consortia
- Kopieren, Scannen - Copying, Scans
- Kurse, Führungen - Courses, Introductions
- Öffnungszeiten - Opening Hours
- DEAL-Info
- DKFZ-Intern - internal only
- Omics IT and Data Management Core Facility
- WHO Collaborating Centers
- Cooperations & Networks
- National Cooperations
- International Cooperations
- Cooperational Research Program with Israel: DKFZ - MOST in Cancer Research
- Program
- Members of the Program Committee
- Call 2018
- Publication Database
- German-Israeli Cancer Research Schools
- Archive
- Heidelberg - Israel, Science and Culture
- Symposium 40 Years of German-Israeli Cooperation
- 35th Anniversary Symposium
- 34th Meeting of the DKFZ-MOST Program
- 40th Anniversary Publication
- 30th Anniversary Publication
- 20th Anniversary Publication
- Flyer - The Cancer Cooperation Program
- List Publications 1976-2004
- Highlight-Projects
- Cooperational Research Program with Israel: DKFZ - MOST in Cancer Research
- Cooperations with industrial companies
- Helmholtz Alliances
- DKFZ PostDoc Network
- Cross Program Topic RNA@DKFZ
- Cross Program Topic Cancer Inflammation
- Cross Program Topic Epigenetics@dkfz
- Cross Program Topic Single Cell Sequencing
- iMed
- INFORM
- Databox
- Baden-Württemberg Cancer Registry
Vector Production & Development Unit (VP&DU)
Introduction
The VP&D Unit supports research-associated groups on two levels:
- Large Scale production of purified virus (vector) stocks for preclinical applications or basic research
- Development and optimization of new production, purification and analytic methods with the aim to establish working protocols for GMP-production of virus(vector) stocks necessary for clinical applications planned in the department F010.
Aim
- High virus yield and concentration
- High purity
- Precise analysis (quantity and quality)
- Good process control
- High safety
- Process development including upscaling
Task
- Standard large-scale production of purified, well characterized virus stocks of wildtype and recombinant parvoviruses.
- Accompanying research for clinical studies.
- Consulting and technical support intern by questions to virus production, purification and analysis.
- Optimization and adaptation of production and purification methods.
Beside providing support for scientists our aim is to establish standard protocols for:
(a) self-replicating wild type and mutant parvoviruses and
(b) recombinant autonomous and adeno-associated parvoviruses carrying foreign therapeutic genes.
These new developments concern:
- the improvement of distinct steps in the production and purification protocols (e.g. evaluation of initial titers for virus production [optimization of per cell output], optimizing transfection procedures [cost factors included], search for suitable cell lines for optimal production),
- up-scaling of the currently used procedures (evaluation of suitable production factories, adaptation to suspension/micro-carrier cultures),
- the establishment of new procedures for virus purification and quality controls (concentration of virus stocks, purification by alternative/additional purification steps [e.g. affinity chromatography], establishment of quality control measurements, (SDS-PAGE, HPLC/FPLC, Empty capsid vs. full capsids by ELISA-techniques),
- adaptation of present materials and methodologies to the production of GMP-grade virus (vector) stocks to support clinical trials
In the establishment of GMP-produced parvovirus, the unit also plays a pivotal role in the interaction of the F010 division with the various organizations involved in the approval and application of procedures for virus (vector) production under GMP-conditions.