Single-cell Open Lab (scOpenLab)

Overview

Single cell sequencing (sc-seq) technologies are evolving fast and become more and more important for many research groups at the DKFZ. The scOpenLab offers assisted access to instrumentation for processing cells and preparing the libraries for sc-seq to all DKFZ groups.

The lab is headed by Jan-Philipp Mallm (j.mallm@dkfz.de) and is located in the Bioquant in rooms 423-425 on the 4th floor.

We offer regular seminars (please see below) to introduce new users to single-cell sequencing applications. After participating in the seminar the systems can be booked online and a training session / detailed project discussion can be arranged.

The scOpenLab also supports setting up new protocols or developing new methods in collaboration with research groups. Here, we focus on providing automated, high-throughput and multi-omic readouts tailored to cancer research.

Available sc-seq readouts

single-nuclei sequencing
© dkfz.de

Currently, the technologies described below are available. Our portfolio of methods and instruments is constantly being updated and expanded in close interactions with the DKFZ groups of the sc-seq@dkfz network. Please contact us if you miss methods important for your work.

 

10x Genomics Chromium system (www.10xgenomics.com)

  • scRNA-seq (3' / 5' counting)
  • scVDJ-seq (+scRNA-seq)
  • scCNV-seq
  • scATAC-seq
  • CITE-seq & cell hashing

 

The preparation of a clean single-cell suspension is key for a successful experiment. We can provide protocols / guidelines for dissociation, nuclei extraction from frozen tissue and nuclei preparation for scATAC-seq. All cell / nuclei suspensions can be analyzed with an automated cell counting system at the scOpenLab prior to loading. 

Encapsulation of cardiomyocytes
© dkfz.de

 

InDrop (www.1cell-bio.com)

  • scRNA-seq (3' counting)
  • Custom scRNA-seq drop-seq assays
  • Cell types with unusual size

 Tapestri (www.missionbio.com)

  • targeted scDNA-seq

 

Customized scRNA-seq assays can be conducted with the inDrop system from 1CellBio. For targeted single-cell sequencing we can provide access to the Tapestri (scDNA-seq) system. Panels for targeted amplification can be designed or directly purchased from the companies - for further information please visit their websites. 

Correlation matrix scATAC-seq
© dkfz.de

 

 

Fluidigm C1 system (www.fluidigm.com)

  • scRNA-seq 
  • scATAC-seq 

 

Instead of capturing cells in droplets the Fluidigm C1 systems traps cells on a chip (IFC) and uses microfluidics to carry out enzymatic reactions. scRNA-seq and scATAC-seq experiments can be run on IFCs with either 96 or 800 capture sites. Multi-omics readouts are also available on the 96-IFCs as well as total RNA assays. 

Single-cell printing
© dkfz.de

Automized on plates

  • scRNA-seq
  • scATAC-seq
  • scHiC
  • scWGBS
  • G&T-seq
  • scNMT-seq (in validation)

Automated workflows are conducted with the Bravo (Agilent) or the Mosquito (TTP Labtech) systems, depending on the reaction volume. Customized protocols can be programmed and run on both machines and we are happy to work with you for setting up custom protocols.

How to get started

1)   Visit our introductory seminar

For all new users we offer a single-cell sequencing seminar covering the different techniques, pros & cons, project design and lab logistics. The introduction is mandatory for all new scOpenLab users.

At the following dates the next mandatory introductions will take place:

  • 14.01.2020  10 – 11 AM, Bioquant, 6th floor, Seminar room 655
  • 18.02.2020  10 – 11 AM, Bioquant, 6th floor, Seminar room 655

If you would like to participate, please send an e-mail to: scopenlab@dkfz.de

2)   Make an appointment with us to discuss your project in detail (optional but highly recommended)

3)   Create an account for booking machines online: https://dkfzppms.inet.dkfz-heidelberg.de/scopenlab/

4)   Book machines for your first experiment and request assistance

For all new users we will offer an introduction on an individual basis for each machine / technique in the lab according to their project.

5)   Run your experiment with a scOpenLab staff member

  

Staff members

Dr. Jan-Philipp Mallm

Dr. Michelle Liberio

Katharina Bauer

Laura Giese

Contact

scOpen Lab (W192)
Bioquant Center, room 423
Im Neuenheimer Feld 267
69120 Heidelberg
Germany
Tel.: +49-6221-54-51321 (lab)
Tel.: +49-6221-54-51322 (office)
e-mail: scOpenLab@dkfz.de

 

Partners & Funding

Selected Publications

Sacma M, PospiechJ , Bogeska R, de Back W, Mallm JP, Sakk V, Soller K , Marka GM, Vollmer A, Karns R, Cabezas-Wallscheid N, Trumpp A, Mendez-Ferrer S, Milsom M , Mulaw MA , Geiger H, Florian MC. (2019) Haematopoietic stem cells in perisinusoidal niches are protected from ageing. Nat Cell Biol, 21, 1309-1320.

Tirier SM, Park J, Preusser F, Amrhein L, Gu Z, Steiger S, Mallm JP, Waschow M, Eismann B, Gut M, Gut IG, Rippe K, Schlesner M, Theis F, Fuchs C, Ball, CR, Glimm H, Eils R, Conrad C. (2019) Pheno-seq – linking visual features and gene expression in 3D cell culture systems. Scientific Rep, 9:12367

Mallm JP, Iskar M, Ishaque N, Klett LC, Kugler SJ, Muino JM, Teif VB, Poos AM, Großmann S, Erdel F, Tavernari D, Koser SD, Schumacher S, Brors B, König R, Remondini D, Vingron M, Stilgenbauer S, Lichter P, Zapatka M, Mertens D, Rippe K. (2019) Linking aberrant chromatin features in chronic lymphocytic leukemia to transcription factor networks. Mol Syst Biol 15:e8339

Becker H, Greve G, Kataoka K, Mallm JP, Duque-Afonso J, Ma T, Niemöller C, Pantic M, Duyster J, Cleary ML, Schüler J, Rippe K, Ogawa S, Lübbert M. (2019) Identification of enhancer of mRNA decapping 4 as a novel fusion partner of MLL in acute myeloid leukemia. Blood Adv 3:761-765.

Kalamakis G, Brüne D, Ravichandran S, Bolz J, Fan W, Ziebell F, Stiehl T, Catalá-Martinez F, Kupke J, Zhao S, Llorens-Bobadilla E, Bauer K, Limpert S, Berger B, Christen U, Schmezer P, Mallm JP, Berninger B, Anders S, Del Sol A, Marciniak-Czochra A, Martin-Villalba A. (2019) Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Cell 176:1407-1419.

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