Cookie Settings

We use cookies to optimize our website. These include cookies that are necessary for the operation of the site, as well as those that are only used for anonymous statistic. You can decide for yourself which categories you want to allow. Further information can be found in our data privacy protection .


These cookies are necessary to run the core functionalities of this website and cannot be disabled.

Name Webedition CMS
Purpose This cookie is required by the CMS (Content Management System) Webedition for the system to function correctly. Typically, this cookie is deleted when the browser is closed.
Name econda
Purpose Session cookie emos_jcsid for the web analysis software econda. This runs in the “anonymized measurement” mode. There is no personal reference. As soon as the user leaves the site, tracking is ended and all data in the browser are automatically deleted.

These cookies help us understand how visitors interact with our website by collecting and analyzing information anonymously. Depending on the tool, one or more cookies are set by the provider.

Name econda
Purpose Statistics
External media

Content from external media platforms is blocked by default. If cookies from external media are accepted, access to this content no longer requires manual consent.

Name YouTube
Purpose Show YouTube content
Name Twitter
Purpose activate Twitter Feeds

Regulation of Cytokinesis


Cytokinesis is the process at the end of mitosis by which mother and daughter cells are separated from one another. It involves the coordination of the cell cycle with multiple cellular processes such as cytoskeleton organisation and membrane fusion machinery. Failure in cytokinesis is linked to aneuploidy and a predisposition to a variety of cancers.

The molecular mechanisms that control cytokinesis are still poorly understood. In budding yeast, the site of cell division is established at early stages of the cell cycle by the deposition of scaffold proteins, called septins, at the mother-daughter boundary (bud neck). The septin ring provides the seeding point for the formation of the acto-myosin ring complex, which drives the separation of the two cell bodies in late anaphase. Animal cells also use a contractile ring system composed of actin and myosin filaments, for division. Although the acto-myosin ring complex is formed at variable stages of the cell cycle depending on the organism, it will not contract until late anaphase. This coordination is important to allow cytokinesis to occur only after full partitioning of the chromosomes between mother and daughter cells.

At the end of mitosis, after the full elongation of the mitotic spindle and separation of the sister chromatids, cyclin-dependent kinase (CDK) activity is down-regulated to allow transition out of mitosis (mitotic exit) and cytokinesis. The protein phosphatase Cdc14 plays a pivotal role during mitotic exit in budding yeast. Through dephosphorylation of key targets, Cdc14 promotes the degradation of the mitotic cyclin Clb2 and accumulation of the CDK inhibitor Sic1. Full activation of Cdc14 requires an active MEN, including the protein kinases Cdc15, Dbf2-Mob1 and Dfb20. Dbf2 and Dbf20 function directly upstream of Cdc14 in the MEN pathway. As a consequence of MEN activation, Cdc14 becomes fully active. Although down-regulation of mitotic CDK activity by the MEN is a pre-requisite for cytokinesis, several lines of evidence suggest that MEN components and Cdc14 have a more direct role in cytokinesis. MEN components such as Mob1 and the kinases Cdc15, Dbf2 and Dbf20 localise at the yeast centrosome in a cell cycle-dependent manner, and in addition appear at the plane of division in late mitosis. MEN deficient mutants fail to complete cytokinesis, even if mitotic CDK activity is down-regulated.

Little is known about how Cdc14 and MEN components regulate cytokinesis. We are systematically studying the function of Cdc14 and MEN components in cell division. In addition, proteomics approaches are employed to identify new components involved in cell division in both budding yeast and mammalian cells.

to top
powered by webEdition CMS