Cookie Settings

We use cookies to optimize our website. These include cookies that are necessary for the operation of the site, as well as those that are only used for anonymous statistic. You can decide for yourself which categories you want to allow. Further information can be found in our data privacy protection .

Essential

These cookies are necessary to run the core functionalities of this website and cannot be disabled.

Name Webedition CMS
Purpose This cookie is required by the CMS (Content Management System) Webedition for the system to function correctly. Typically, this cookie is deleted when the browser is closed.
Name econda
Purpose Session cookie emos_jcsid for the web analysis software econda. This runs in the “anonymized measurement” mode. There is no personal reference. As soon as the user leaves the site, tracking is ended and all data in the browser are automatically deleted.
Statistics

These cookies help us understand how visitors interact with our website by collecting and analyzing information anonymously. Depending on the tool, one or more cookies are set by the provider.

Name econda
Purpose Statistics
External media

Content from external video platforms is blocked by default. If cookies from external media are accepted, access to this content no longer requires manual consent.

Name Youtube
Purpose External media

Developmental specification of adult stem cells and the study of how key biological processes differ in these cells between the embryonic and adult state

Pluripotent cell lines can be differentiated into various blood cell types via the process of embryoid body formation. The burst-forming erythroid unit (BFU-e) comprises a type of red cell progenitor while the colony forming unit granulocyte-macrophage (CFU-GM) is a bi-potent myeloid progenitor cell capable of making both granulocytes and macrophages.
© dkfz.de

During embryonic development, HSCs are generated from pluripotent cells in a series of progressive differentiation steps. This process can be modeled in vitro by directing the differentiation of pluripotent cell lines, such as embryonic stem (ES) cells or induced pluripotent stem cells (iPSCs), towards the formation of the hematopoietic lineages. The in vitro specification of hematopoietic lineages from ES cells and patient-specific iPSCs closely mimics the process via which HSCs are generated during development, and holds enormous potential for disease modeling and for the eventual scalable production of differentiated cells for regenerative therapy. However, a number of differences exist between the normal developmental process and the in vitro differentiation procedure. The most notable of these differences is the current inability to generate in vitro, HSCs that have the same functional properties as their in vivo specified counterparts. One of the current focuses of the Experimental Hematology group is to better understand the important molecular and biological differences between hematopoietic specification in vitro and in vivo, and to attempt to apply this knowledge towards developing better methodologies for the generation of functional HSCs from ES cells and iPSCs.

Key Publications on pluripotent cell lines and HSC specification

  1. §Müller L.U.W., §Milsom M.D., Harris C.E., Vyas R., Brumme K.M., Parmar K., Moreau L.A., Schambach A., Park I.H., London W.B., Strait K., D’Andrea A., Daley G.Q. and Williams D.A. (2012). Overcoming reprogramming resistance of Fanconi anemia cells. Blood. 119 (23): pp5449-57. §Equal contribution.
     
  2. Ghiaur G., Ferkowicz M.J., Milsom M.D., Bailey J., Cancelas J.A., Yoder M.C. and Williams D.A. (2008). Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of the fetal liver with hematopoietic progenitor cells. Blood, 111 (7): pp3313-21.

to top
powered by webEdition CMS