Selected Publications

  • Boutros M., Paricio, N., Strutt, D., Mlodzik, M. (1998). Dishevelled activates JNK
    and discriminates between JNK pathways in planar polarity and wingless signaling.
    Cell 94:109-18.
  • Boutros, M., Mihaly, J., Bouwmeester, T., Mlodzik, M. (2000). Signaling specificity by
    Frizzled receptors in Drosophila. Science 288:1825-8.
  • Boutros, M., Kiger, A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S., HD
    FlyArray, Paro, R., Perrimon, N. (2004). Genome-wide RNAi Analysis of Cell Growth
    and Viability in Drosophila. Science 303:832-835.
  • Bartscherer, K., Pelte, N., Ingelfinger, D., Boutros, M. (2006). Secretion of Wnt ligands
    requires Evi, a conserved transmembrane protein. Cell 125:523-33.
  • Boutros, M., L. Bras, W. Huber. (2006). Analysis of cell-based RNAi screens.
    Genome Biology 7:R66
  • Boutros M., Ahringer, J. (2008). The art and design of genetic screens: RNA
    interference. Nature Reviews Genetics 9:554-566.
  • Horn, T., Sandmann, T., Fischer, B., Axelsson, E., Huber, W., Boutros, M. (2011).
    Mapping of signalling networks through synthetic genetic interaction analysis by RNAi.
    Nature Methods 8:341-6.
  • Gross, J., Chaudhary, V., Bartscherer, K., Boutros, M. (2012). Active Wnt proteins are
    secreted on exosomes. Nature Cell Biology 14:1036-45.
  • Laufer, C., Fischer, B., Billmann, M., Huber, W., Boutros, M. (2013). Mapping genetic
    interactions in human cancer cells with RNAi and multiparametric phenotyping.
    Nature Methods
    10:427–431.
  • Voloshanenko, O., Erdmann, G., Dubash, T.D., Augustin, I., Metzig, M., Moffa, G.,
    Hundsrucker, C., Kerr, G., Sandmann, T., Anchang, B., Demir, K., Boehm, C., Leible,
    S., Ball, C.R., Glimm, H., Spang, R., Boutros, M. (2013). Wnt secretion is required to
    maintain high levels of Wnt activity in colon cancer cells.
    Nature Communications 4:2610.
  • Fischer, B., Sandmann, T., Horn, T., Billmann, M., Chaudhary, V., Huber, W., Boutros,
    M (2015). A Map of Directional Genetic Interactions in a Metazoan Cell.
    eLife 4 doi:10.7554/eLife.05464.

Complete List of Publications

Chong, J.A., Tapia-Ramirez, J., Kim, S., Toledo-Aral, J.J., Zheng, Y., Boutros, M.C., Altshuller, Y.M., Frohman, M.A., Kraner, S.D., Mandel, G. (1995). REST: A Mammalian Silencer Protein That Restricts Sodium Channel Gene Expression to Neurons. Cell 80:949-57.

Boutros M., Paricio, N., Strutt, D., Mlodzik, M. (1998). Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. Cell 94:109-18.

Paricio N., Feiguin, F., Boutros, M., Eaton, S., Mlodzik, M. (1999). The Drosophila STE20-like kinase misshapen is required downstream of the Frizzled receptor in planar polarity signaling. EMBO Journal 18:4669-78.

Boutros M., Mlodzik, M. (1999). Dishevelled: at the crossroads of divergent intracellular signaling pathways. Mechanisms of Development 83:27-37.

Wilson, A.C., Boutros, M., Johnson, K.M., Herr, W. (2000). HCF Amino- and Carboxy-Terminal Subunit Association through Two Separate Sets of Interaction Modules: Involvement of Fibronectin Type 3 Repeats. Molecular and Cellular Biology 20:6721-30.

Boutros M., Perrimon, N. (2000). Drosophila genome takes flight. Nature Cell Biology 2:E53-4.

Boutros, M., Mihaly, J., Bouwmeester, T., Mlodzik, M. (2000). Signaling specificity by Frizzled receptors in Drosophila. Science 288:1825-8.

Moon R.T., Bowerman, B., Boutros, M., Perrimon, N. (2002). The promise and perils of Wnt signaling through beta-catenin. Science 296:1644-6.

Boutros, M., Agaisse, H., Perrimon, N. (2002). Sequential activation of signaling pathways during innate immune responses in Drosophila. Developmental Cell 3:711-22.  

Agaisse, H., Petersen, U.-M., Boutros, M., Mathey-Prevot, B., Perrimon, N. (2003). Signaling Role of Hemocytes in Drosophila JAK/STAT-Dependent Response to Septic Injury. Developmental Cell 5:441-50.

Hild, M., Beckmann, B., Haas, S., Koch, B., Solovyev, V., Busold, C., Fellenberg, K., Boutros, M., Vingron, M., Sauer, F., Hoheisel, J. D., Paro, R. (2003). An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome. Genome Biology 5:R3.

Boutros, M., Kiger, A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S., HD FlyArray, Paro, R., Perrimon, N. (2004). Genome-wide RNAi Analysis of Cell Growth and Viability in Drosophila. Science 303:832-835.

Gesellchen V., Boutros, M. (2004). Managing the genome: microRNAs in Drosophila. Differentiation 72:74-80.

Kuttenkeuler D., Boutros, M. (2004). Genome-wide RNAi as a route to gene function in Drosophila. Brief Funct Genomic Proteomic 3:168-76.

Choe, S. E., Boutros, M., Michelson, A.M., Church, G.M., Halfon, M.S. (2005). Preferred analysis methods for Affymetrix GeneChips revealed by a wholly-defined control dataset. Genome Biology 6:R16.

Armknecht, S., Boutros, M., Kiger, A., Nybakken, K., Mathey-Prevot, B., Perrimon, N.. (2005). High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymology 392:55-73.

Arziman, Z., Horn, T., Boutros, M. (2005). E-RNAi: A web application to design optimized RNAi constructs. Nucleic Acids Research 33:W582-88.

Muller, P., Kuttenkeuler, D., Gesellchen, V., Zeidler, M., Boutros, M. (2005). Identification of JAK/STAT signaling components by genome-wide RNAi. Nature 436:871-5.  

Gesellchen, V., Kuttenkeuler, D., Steckel, M., Pelte, N., Boutros, M. (2005). An RNAi screen identified a Role for IAP2 in Innate Immune Response in Drosophila. EMBO reports 6:979-984.

Fuchs, F., Boutros, M. (2006). Cellular phenotyping by RNAi. Brief Funct Genomic Proteomic 5:52-6.

Dasgupta, R., Boutros, M., Perrimon, N. (2005) Drosophila Wnt/Fz pathways. Sci STKE 2005:cm5.

Flockhart, I., Booker, M., Kiger, A., Boutros, M., Armknecht, S., Ramadan, N., Richardson, K., Xu, A., Perrimon, N., Mathey-Prevot, B. (2006). FlyRNAi: the Drosophila RNAi screening center database. Nucleic Acids Research 34:D489–94.

Bartscherer, K., Pelte, N., Ingelfinger, D., Boutros, M. (2006). Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 125:523-33.

Echeverri C.J., Beachy. P.A., Baum, B., Boutros, M., Buchholz, F., Chanda, S.K., Downward, J., Ellenberg, J., Fraser, A.G., Hacohen, N., Hahn, W.C., Jackson, A.L., Kiger, A., Linsley, P.S., Lum, L., Ma, Y., Mathey-Prevot, B., Root, D.E., Sabatini, D.M., Taipale, J., Perrimon, N., Bernards, R.  (2006). Minimizing the risk of reporting false positives in large-scale RNAi screens. Nature Methods 3:777-9.

Boutros, M., L. Bras, W. Huber. (2006). Analysis of cell-based RNAi screens. Genome Biology 7:R66Horn, T., Arziman, Z., Berger, J., Boutros, M. (2007). GenomeRNAi: a database for cell-based RNAi phenotypes. Nucleic Acid Research 35:D492-7

Eulalio, A., Rehwinkel, J., Stricker, M., Huntzinger, E., Yang, S.F., Doerks, T., Dorner, S., Bork, P., Boutros, M., Izaurralde, E. (2007). Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Genes and Development 21:2558-2570.

Goto, A., Matsushita, K., Gesellchen, V., El Chamy L., Kuttenkeuler, D., Takeuchi, O., Hoffmann, J.A., Akira, S., Boutros, M., Reichhart, J.M. (2008). Akirins are highly conserved nuclear proteins required for NF-kappaB-dependent gene expression in Drosophila and mice. Nature Immunology 9:97-10.

Stielow, B., Sapetschig, A., Krueger, I., Kunert, N., Brehm, A., Boutros, M., Suske, G. (2008). Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen. Molecular Cell 29:742-754.

Boutros M., Ahringer, J. (2008). The art and design of genetic screens: RNA interference. Nature Reviews Genetics 9:554-566.

Mueller P., Boutros, M., Zeidler, M. (2008). Identification of JAK/STAT pathway regulators-Insights from RNAi screens. Seminar Cell Dev Biol 19:360-369.

Steinbrink, S., Boutros, M. (2008). RNAi screening in cultured Drosophila cells. Methods Molecular Biology 420:139-1353.

Bartscherer, K., Boutros, M. (2008). Regulation of Wnt protein secretion and its role in gradient formation. EMBO reports 9:977-982.

Adell, T., Salo, E., Boutros, M., Bartscherer, K. (2009). Smed-Evi/Wntless is required for b-catenin-dependent and –independent processes during planarian regeneration. Development 136:905-910.

Gauhar, Z., Sun, L.V., Hua, S., Mason, C.E., Fuchs, F., Li, T.R., Boutros, M., White, K.P. (2009). Genomic mapping of binding regions for the ecdysone receptor protein complex. Genome Research 19:1006-1013.

Simons, M., Gault, W.J., Gotthardt, D., Rohatgi, R., Klein, T.J., Shao, Y., Lee, H.J., Wu, A.L., Fang, Y., Satlin, L.M., Dow, J.T., Chen, J., Zheng, J., Boutros, M., Mlodzik, M. (2009). Electrochemical cues regulate assembly of the Frizzled/Dishevelled complex at the plasma membrane during planar epithelial polarization. Nature Cell Biology 11:286-94.

Farfsing, A., Engel, F., Seiffert, M., Hartmann, E., Ott, G., Rosenwald, A., Stilgenbauer, S., Döhner, H., Boutros, M., Lichter, P., Pscherer, A. (2009). Gene knockdown studies revealed CCDC50 as a candidate gene in mantle cell lymphoma and chronic lymphocytic leukemia. Leukemia 11:2018-26.

Davidson, G., Shen, J., Huang, Y.L., Su, Y., Karaulanov, E., Bartscherer, K., Hassler, C., Stannek, P., Boutros, M., Niehrs, C. (2009). Cell cycle control of Wnt receptor activation. Developmental Cell 17:788-99.

Kuttenkeuler, D., Pelte, N., Ragab, A., Gesellchen, V., Schneider, L., Blass, C., Axelsson, E., Huber, W., Boutros, M. (2010). A Large-Scale RNAi Screen Identifies Deaf1 as a Regulator of Innate Immune Responses in Drosophila. Journal of Innate Immunity 2:181-194.

Gilsdorf, M., Horn, T., Arziman, Z., Pelz, O., Kiner E., Boutros, M. (2010). GenomeRNAi: a database for cell-based RNAi phenotypes. 2009 Update. Nucleic Acids Research 38:D448-52.

Mleczko-Sanecka, K., Casanovas, G., Ragab, A., Breitkopf, K., Muller, A., Boutros, M., Dooley, S., Hentze, M.W., Muckenthaler, M.U. (2010). SMAD7 controls iron metabolism as a potent inhibitor of hepcidin expression. Blood 115:2657-65.

Cruciat, C.M., Ohkawara, B., Acebron, S.P., Karaulanov, E., Reinhard, C., Ingelfinger, D., Boutros, M., Niehrs, C. (2010). Requirement of prorenin receptor an vacuolar H+-ATPase-mediated acidification for Wnt signaling. Science 327:459-63.

Pau, G., Fuchs, F., Sklyar, O., Boutros, M., Huber, W. (2010). EBImage - an R package for image processing with applications to cellular phenotypes. Bioinformatics 26:979-81.

Falschlehner, C., Steinbrink, S., Erdmann, G., Boutros, M. (2010). High-throughput RNAi screening to dissect cellular pathways: a how-to guide. Biotechnology Journal 5:368-76.

Gobert, V., Osman, D., Bras, S., Auge, B., Boube, M., Bourbon, H.M., Horn, T., Boutros, M., Haenlin, M., Waltzer, L. (2010). A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/RUNX-activated transcription in Drosophila. Molecular Cellular Biology 30:2837-48.

Pelz., O., Gilsdorf, M., Boutros, M. (2010). web cellHTS: A web application for the analysis of high-throughput screening data. BMC Bioinformatics 11:185.

Horn, T., Boutros, M. (2010). E-RNAi: a web application for the multi-species design of RNAi reagents—2010 update. Nucleic Acids Research 38:W332-9.

Saj, A., Arziman, Z., Stempfle, D., van Belle, W., Sauder, U., Horn, T., Dürrenberger, M., Paro, R., Boutros, M., Merdes, G. (2010). A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Developmental Cell 18:862-76.

Leber, B., Maier, B., Fuchs, F., Chi, J., Riffel, P., Anderhub, S., Wagner, L., Ho, A.D., Salisbury, J.L., Boutros, M., Krämer, A. (2010). Proteins required for centrosome clustering in cancer cells. Science Translational Medicine 2:33ra38.

Horn, T., Sandmann, T., Boutros, M. (2010).  Design and evaluation of genome-wide libraries for RNAi screens. Genome Biology 11:R61.

Fuchs, F., Pau, G., Kranz, D., Sklyar, O., Budjan, C., Steinbrink, S., Horn, T., Pedal, A., Huber, W., Boutros, M. (2010). Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Molecular Systems Biology 6:370.

Buechling, T., Bartscherer, K., Ohkawara, B., Chaudhary, V., Spirohn, K., Niehrs, C., Boutros, M. (2010).  Wnt/Frizzled signaling requires dPRR, the Drosophila homolog of the prorenin receptor. Current Biology 20:1263-1268.

Niehrs, C., Boutros, M. (2010). Trafficking, Acidification and Growth Factor Signaling. Science Signaling 3:pe26.

Müller, H., Schmidt, D., Steinbrink, S., Mirgorodskaya, E., Lehmann, V.,  Habermann, K., Dreher, F., Gustavsson, N., Kessler, T., Lehrach, H., Herwig, R., Gobom, J., Ploubidou, A., Boutros, M., Lange, B.M. (2010). Proteomic and functional analysis of the mitotic Drosophila centrosome. EMBO Journal 29:3344-57.

Gilboa, L., Boutros, M. (2010). Celebrating 100 years of Drosophila research. EMBO reports 11:724-726.

Metzig, M., Nickles, D., Boutros, M. (2011). Large-scale RNAi screens to dissect TNF and NF-κB signaling pathways. Adv Exp Med Biol. 691:131-9.

Metzig, M., Nickles, D., Falschlehner, C., Lehmann-Koch, J., Straub, B.K., Roth, W., Boutros, M. (2011). An RNAi screen identifies USP2 as a factor required for TNF-α induced NF-κB signaling. Int Journal of Cancer 29:607-18.

Liang, J., Fu, Y., Cruciat, C.M., Jia, S., Wang, Y., Tong, Z., Tao, Q., Ingelfinger, D., Boutros, M., Meng, A., Niehrs, C., Wu, W. (2011). Transmembrane Protein 198 Promotes LRP6 Phosphorylation and Wnt Signaling Activation. Molecular Cellular Biology 31:2577-90.

Huhn, S., Ingelfinger, D., Bermejo, J.L., Bevier, M., Pardini, B., Naccarati, A., Steinke, V., Rahner, N., Holinski-Feder, E., Morak, M., Schackert, H.K., Görgens, H., Pox, C.P., Goecke, T., Kloor, M., Loeffler, M., Büttner, R., Vodickova, L., Novotny, J., Demir, K., Cruciat, C.M., Renneberg, R., Huber, W., Niehrs, C., Boutros, M., Propping, P., Vodièka, P., Hemminki, K., Försti, A. (2011). Polymorphisms in CTNNBL1 in relation to colorectal cancer with evolutionary implications. Int J Mol Epidemiol Genet. 2:36-50.

Kondylis, V., Tang, Y., Fuchs, F., Boutros, M., Rabouille, C. (2011). Identification of ER Proteins involved in the Functional Organisation of the Early Secretory Pathway in Drosophila Cells by a Targeted RNAi Screen. PLoS One 6:e17173.

Ragab, A., Buechling, T., Gesellchen, V., Spirohn, K., Boettcher, A.-L., Boutros, M. (2011). Drosophila Ras/MAPK signalling regulates innate immune responses in immune and intestinal stem cells. EMBO Journal 30:1123-36

Horn, T., Sandmann, T., Fischer, B., Axelsson, E., Huber, W., Boutros, M. (2011). Mapping of signalling networks through synthetic genetic interaction analysis by RNAi. Nature Methods 8:341-6.

Axelsson, E., Sandmann, T., Horn, T., Boutros, M., Huber, W., Fischer, B. (2011).  Extracting quantitative genetic interaction phenotypes from matrix combinatorial RNAi. BMC Bioinformatics 12:342.

Sandmann, T., Vogg, M.C., Owlarn, S., Boutros, M., Bartscherer, K. (2011). The head-regeneration transcriptome of the planarian Schmidtea mediterranea. Genome Biology 12:R76

Zacharogianni, M., Kondylis, V., Tang, Y., Farhan, H., Xanthakis, D., Fuchs, F., Boutros, M., Rabouille, C. (2011). ERK7 is a negative regulator of protein secretion in response to amino acid starvation. EMBO Journal 30:3684-700.

Glinka, A., Dolde, C., Kirsch, N., Huang, Y.L., Kazanskaya, O., Ingelfinger, D., Boutros, M., Cruciat, C.-M., Niehrs, C. (2011). LGR4 and LGR5 are R-spondin receptors mediating Wnt/beta-catenin and Wnt/PCP signaling. EMBO reports 12:1055-61.

Gilbert, D.F., Erdmann, G., Zhang, X., Fritzsche, A., Demir, K., Jaedicke, A., Muehlenberg, K., Wanker, E.E., Boutros, M. (2011). A Novel Multiplex Cell Viability Assay for High-Throughput RNAi Screening. PLoS One 6:e28338.

Buechling, T., Boutros, M. (2011). Wnt signaling signaling at and above the receptor level. Curr Top Dev Biol. 97:21-53.

Buechling, T., Chaudhary, V., Spirohn, K., Weiss, M., Boutros, M. (2011). p24 proteins are required for secretion of Wnt ligands. EMBO reports, 12:1265-72.

Augustin, I., Goidts, V., Bongers, A., Kerr, G., Vollert, G., Radlwimmer, B., Hartmann, C., Herold-Mende, C., Reifenberger, G., von Deimling, A., Boutros, M. (2012). The Wnt secretion protein Evi/Gpr177 promotes glioma tumorigenesis. EMBO Molecular Medicine 4:38-51.

Shamu, C., Wiemann, S., Boutros, M. (2012). On target: Call for a public repository for large-scale RNAi experiments. Nature Cell Biology, 14:115.

Sandmann, T., Boutros, M. (2012). Screens, Maps and Networks: from genome sequences to personalized medicine. Current Opinions in Genetics and Development 22:36-44.

Falschlehner, C., Boutros, M. (2012). Innate immunity: regulation of caspases by IAP-dependent ubiquitylation. EMBO Journal 31:2750-2.

Icardi, L., Moria, R., Gesellchen, V., Eyckerman, S., De Cauwer, L., Verhelst, J., Vercauterene, K., Saelens, X., Meulemane, P., Leroux-Roelse, G., De Bosscher, K., Boutros, M., Tavernier, J. (2012). Sin3a repressor complex is a master regulator of STAT transcriptional activity. Proc. Natl. Acad. Sci. 109:12058-63.

Nickles, D., Falschlehner, C., Metzig, M., Boutros, M. (2012). A genome-wide RNAi screen identifies CASP4 as a factor required for tumor necrosis factor alpha signaling. Molecular and Cellular Biology 32:3372-81.

Demir, K., Boutros, M. (2012). Cell perturbation screens for target identification by RNAi. Methods Molecular Biology 910:1-13.

Stender, J.D., Pascual, G., Liu, W., Kaikkonen, M.U., Do, K., Spann, N.J., Boutros, M., Perrimon, N., Rosenfeld, M.G., Glass, C.K. (2012). Control of proinflammatory gene programs by regulated trimethylation and demethylation of histone H4K20. Molecular Cell 48:28–38.

Petrakis, S., Raskó, T., Russ, J., Stroedicke, M., Riechers, S., Friedrich, R. P., Möller, A., Reinhardt, A., Vinayagam, A., Schaefer, M., Boutros, M., Tricoire, H., Andrade-Navarro, M. A., Wanker, E. E. (2012). Identification of Human Proteins that Modify Misfolding and Proteotoxicity of Pathogenic Ataxin-1. PLoS Genetics 8:e1002897.

Gross, J., Chaudhary, V., Bartscherer, K., Boutros, M. (2012). Active Wnt proteins are secreted on exosomes. Nature Cell Biology 14:1036-45.

Horn, T., Boutros, M. (2012). Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors. Methods Molecular Biology 942:315-46.

Brechmann, M., Mock, T., Nickles, D., Kiessling, M., Weit, N., Breuer, R., Müller, W., Wabnitz, G., Frey, F., Nicolay, J.P., Booken, N., Samstag, Y., Klemke, C.D., Herling, M., Boutros, M., Krammer, P.H., Arnold, R. (2012). A PP4 Holoenzyme Balances Physiological and Oncogenic Nuclear Factor-Kappa B Signaling in T Lymphocytes. Immunity 37:697-708.

Page, R.M., Münch, A., Horn, T., Kuhn, P.H., Colombo, A., Reiner, O., Boutros, M., Steiner, H., Lichtenthaler, S.F., Haass, C. (2012). Loss of PAFAH1B2 Reduces Amyloid-β Generation by Promoting the Degradation of Amyloid Precursor Protein C-Terminal Fragments. Journal of Neuroscience 32:18204-18214.

Erdmann, G., Volz, C., Boutros, M. (2012). Systematic approaches to dissect biological processes in stem cells by image-based screening. Biotechnology Journal 7:768-78.

Schmidt, E.E., Pelz, O., Buhlmann, S., Kerr, G., Horn, T., Boutros, M. (2013). GenomeRNAi: a database for cell-based and in vivo RNAi phenotypes, 2013 update. Nucleic Acids Research 41:D1021–D1026.

Kuhns, S., Schmidt, K.N., Reymann, J., Gilbert, D.F., Neuner, A., Hub, B., Carvalho, R., Wiedemann, P., Zentgraf, H., Erfle, H., Klingmüller, U., Boutros, M., Pereira, G. (2013). The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis. Journal of Cell Biology 200:505–522.

Laufer, C., Fischer, B., Billmann, M., Huber, W., Boutros, M. (2013). Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping. Nature Methods 10:427–431.

Gross, J.C., Boutros, M., (2013). Secretion and extracellular space travel of Wnt proteins. Current Opinions in Genetics and Development 23:385-390.

Fukuyama, H., Verdier, Y., Guan, Y., Makino-Okamura, C., Shilova, V., Liu, X., Maksoud, E., Matsubayashi, J., Haddad, I., Spirohn, K., Ono, K., Hetru, C., Rossier, J., Ideker, T., Boutros, M., Vinh, J., Hoffmann, J.A. (2013). Landscape of protein-protein interactions in Drosophila immune deficiency signaling during bacterial challenge. Proc. Natl. Acad. Sci.  110:10717-22.

Serysheva, E., Berhane, H., Grumolato, L., Demir, K., Balmer, S., Bodak, M., Boutros, M., Aaronson, S., Mlodzik, M., Jenny, A. (2013). Wnk kinases are positive regulators of canonical Wnt/b-catenin signaling. EMBO reports 14:718-25.

Augustin, I., Gross, J., Baumann, D., Korn, C., Kerr, G., Grigoryan, T., Mauch, C., Birchmeier, W., Boutros, M. (2013). Loss of epidermal Evi/Wls results in a phenotype resembling psoriasiform dermatitis. Journal of Experimental Medicine 210:1761-77.

Börner, K., Niopek, D., Cotugno, G., Kaldenbach, M., Pankert, T., Willemsen, J., Zhang, X., Mockenhaupt, S., Schürmann, N., Serva, A, Hiet, M.-S., Wiedtke, E., Castoldi, M., Bauer, A., Starkuviene, V., Erfle, H., Schnitzler, P., Muckenthaler, M., Gilbert, D., Bartenschlager, R., Boutros, M., Binder, M., Streetz, K., Kräusslich, H.-G., Grimm, D. (2013). Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines. Nucleic Acids Research 41:e199.

Heigwer, F., Kerr, G., Walther, N., Glaeser, K., Pelz, O., Breinig, M., Boutros, M. (2013). E-TALEN: a web tool to design TALENs for genome engineering. Nucleic Acids Research 41:e190

Demir, K., Kirsch, N., Beretta, C.A., Erdmann, G., Ingelfinger, D., Moro, E., Argenton, F., Carl, M., Niehrs, C., Boutros, M. (2013). RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Reports 4:1224-34.

Voloshanenko, O., Erdmann, G., Dubash, T.D., Augustin, I., Metzig, M., Moffa, G., Hundsrucker, C., Kerr, G., Sandmann, T., Anchang, B., Demir, K., Boehm, C., Leible, S., Ball, C.R., Glimm, H., Spang, R., Boutros, M. (2013). Wnt secretion is required to maintain high levels of Wnt activity in colon cancer cells. Nature Communications 4:2610.

Zhang, X., Boutros, M. (2013). A novel phenotypic dissimilarity method for image-based high-throughput screens. BMC Bioinformatics 14:336.

Mleczko-Sanecka, K., Roche, F., da Silva, A. R., Call, D., D'Alessio, F., Ragab, A., Lapinski, F.E., Ummani, R., Korf, U., Oakes, C.C., Damm, G., D’Alessandro, L., Klingmüller, U., King, P.D., Boutros, M., Hentze, M.W., Muckenthaler, M. U. (2014). Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to the proliferative Ras/RAF and the nutrient-dependent mTOR signaling pathways. Blood 123:1574-85.

Kranz, D., Boutros, M. (2014). A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. EMBO Journal 33:181-97.

Heigwer, F., Kerr, G., Boutros, M. (2014). E-CRISP: fast CRISPR target site identification. Nature Methods 11:122-3.

Jankovics, F., Henn, L., Bujna, A., Vilmos, P., Spirohn, K., Boutros, M., Erdélyi, M. (2014). Functional analysis of the Drosophila embryonic germ cell transcriptome by RNA interference. PLoS ONE 9:e98579.

Kumar, S., Igman, M., Patel, T.R., Trageser, B., Gross, J.C., Rahm, K., Boutros, M., Gradl, D., Steinbeisser, H., Holstein, T., Stetefeld, J., Ozbek, S. Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of wnt signaling activity. BMC Biology 12:44.

Korn, C., Scholz, B., Hu, J., Srivastava, K., Wojtarowicz, J., Arnsperger, T., Adams, R.H., Boutros, M., Augustin, H.G., Augustin, I. (2014). Endothelial cell-derived non-canonical Wnt ligands control vascular pruning in angiogenesis. Development 141:1757-1766.

Laufer, C., Fischer, B., Huber, W., Boutros, M. (2014). Measuring genetic interactions in human cells by RNAi and imaging. Nature Protocols 9:2341–2353.

Zhou, J., Florescu, S., Boettcher, A.-L., Luo, L., Dutta, D., Kerr, G., Cai, Y., Edgar, B. A., Boutros, M. (2015). Dpp/Gbb signaling is required for normal intestinal regeneration during infection. Developmental Biology 399:189-203.

Khandelwal, N., Breinig, M., Speck, T., Michels, T., Kreutzer, C., Sorrentino, A., Sharma, A. K., Umansky, L., Conrad, H., Poschke, I., Offringa, R., König, R., Bernhard, H., Machlenkin, A., Boutros, M., Beckhove, P. (2015). A high-throughput RNAi screen for detection of immune-checkpoint molecules that mediate tumor resistance to cytotoxic T lymphocytes. EMBO Molecular Medicine 7:450-63.

Guida, C., Altamura, S., Klein, F. A., Galy, B., Boutros, M., Ulmer, A. J., Hentze, M. W., Muckenthaler, M. U. (2015). A novel inflammatory pathway mediating rapid hepcidin-independent hypoferremia. Blood 125:2265-75.

Fischer, B., Sandmann, T., Horn, T., Billmann, M., Chaudhary, V., Huber, W., Boutros, M. (2015). A Map of Directional Genetic Interactions in a Metazoan Cell. eLife 4 doi: 10.7554/eLife.05464.

Tiebe, M., Lutz, M., De La Garza1, A., Buechling, T., Boutros, M., Teleman, A.A. (2015). REPTOR and REPTOR-BP regulate organismal metabolism and transcription downstream of mTORC1. Developmental Cell 33:272-84.

Betge, J., Kerr, G., Miersch, T., Leible, S., Erdmann, G., Galata, C.L., Zhan, T., Gaiser, T., Post, S., Ebert, M.P., Horisberger, K., Boutros, M. (2015). Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples. PLoS ONE 10:e0127146.

Erdmann, G., Suchanek, M., Horn, P., Graf, F., Volz, C., Horn, T., Zhang, X., Wagner, W., Ho, A.D., Boutros, M. (2015). Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening. Genome Medicine 7:46.

Brunk, F., Augustin, I., Meister, M., Boutros, M., Kyewski, B. (2015). Thymic Epithelial cells are a nonredundant source of Wnt ligands for thymus development. Journal of Immunology 195:5261-5271.

Boutros, M., Heigwer, F., Laufer, C. (2015). Microscopy-based high content screening. Cell 163:1314-1325.

Zhan, T., Boutros, M. (2016). Towards a compendium of essential genes – from model organisms to synthetic lethality in cancer cells. Critical Reviews in Biochemistry and Molecular Biology 51:74-85.

Winter, J., Breinig, M., Heigwer, F., Brügemann, D., Leible, S., Pelz, O., Zhan, T., Boutros, M. (2016). caRpools: An R package for exploratory data analysis and documentation of pooled CRISPR/Cas9 screens. Bioinformatics 32:632-4.

Breinig, M., Klein, F., Huber, W., Boutros, M. (2015). A chemical-genetic interaction map of small molecules using high-throughput imaging in cancer cells. Molecular Systems Biology 11:846.

Scholz, B., Korn, C., Wojtarowicz, J., Mogler, C., Augustin, I., Boutros, M., Niehrs, Augustin, H.G. (2016). Endothelial RSPO3 controls vascular stability and pruning through non-canonical WNT/Ca2+/NFAT signaling. Developmental Cell 36:79-93.

Lamiable, O., Kellenberger, C., Kemp, C., Troxler, L., Pelte, N., Boutros, M., Marques, J.T., Daeffler, L., Hoffmann, J.A., Roussel, A., Imler, J-L. (2016). The cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila. Proc. Natl. Acad. Sci 13:698-703.

Li, X., Chatterjee, N., Spirohn, K., Boutros, M., Bohmann, D. (2016).  Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes. Scientific Reports 6:21455.

Schäfer, M., Oeing, C.U., Rohm, M., Baysal-Temel, E., Lehmann, L.H., Bauer, R., Volz, H.C., Boutros, M., Sohn, D., Sticht, C., Gretz, N., Eichelbaum, K., Werner, T., Hirt, M.N., Eschenhagen, T., Müller-Decker, K., Strobel, O., Hackert, T., Krijgsveld, J., Katus, H.A., Berriel Diaz, M., Backs, J., Herzig, S. (2016). Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia. Molecular Metabolism 5:67-78.

Billmann, M., Horn, T., Fischer, B., Sandmann, T., Huber, W., Boutros, M. (2016). A genetic interaction map of cell cycle regulators. Molecular Biology Cell 27:1397-1407.

Boutros, M., Niehrs, C. (2016). Sticking around: short-range activity of Wnt ligands. Developmental Cell 36:485-6.

Tsokanos, F.F., Albert, M.A., Demetriades, C., Spirohn, K., Boutros, M., Teleman, A. A. (2016). eIF4A inactivates TORC1 in response to amino acid starvation. EMBO Journal. 35:1058-76.

Moffa, G., Erdmann, G., Voloshanenko, O., Hundsrucker, C., Sadeh, M.J., Boutros, M., Spang, R. (2016). Refining Pathways: A Model Comparison Approach. PLoS One 11:e0155999.

Chatterjee, N., Tian, M., Spirohn, K., Boutros, M., Bohmann, D. (2016). Keap1-Independent Regulation of Nrf2 Activity by Protein Acetylation and a BET Bromodomain Protein. PLoS Genetics 12:e1006072.

Heigwer, F., Zhan, T., Breinig, M., Winter, J., Brügemann, D., Leible, S., Boutros, M. (2016). CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries. Genome Biology 17:55.

Gilbert, D.F., Boutros, M. (2016). A Protocol for a High-Throughput Multiplex Cell Viability Assay. Methods Molecular Biology 1470:75-84.

Glaeser, K., Boutros, M., Gross, J.C. (2016). Biochemical Methods to Analyze Wnt Protein Secretion. Methods Molecular Biology 1481:17-28.

Konotop, G., Bausch, E., Nagai, T., Turchinovich, A., Becker, N., Benner, A., Boutros, M., Mizuno, K., Krämer, A., Raab, M.S. (2016). Pharmacological inhibition of centrosome clustering by slingshot-mediated cofilin activation and actin cortex destabilization. Cancer Research 76:6690-6700

Costanzo, M., VanderSluis, B., Koch, E.N., Baryshnikova, A., Pons, C., Tan, G., Wang,, W., Usaj, M., Hanchard, J., Lee, S.D., Pelechano, V., Styles, E.B., Billmann, M., van Leeuwen, J., van Dyk, N., Lin, Z.Y., Kuzmin, E., Nelson, J., Piotrowski, J.S., Srikumar, T., Bahr, S., Chen, Y., Deshpande, R., Kurat, C.F., Li, S.C., Li, Z., Usaj, M.M., Okada, H., Pascoe, N., San Luis, B.J., Sharifpoor, S., Shuteriqi, E., Simpkins, S.W., Snider, J., Suresh, H.G., Tan, Y., Zhu, H., Malod-Dognin, N., Janjic, V., Przulj, N., Troyanskaya, O.G., Stagljar, I., Xia, T., Ohya, Y., Gingras, A.C., Raught, B., Boutros, M., Steinmetz, L.M., Moore, C.L., Rosebrock, A.P., Caudy, A.A., Myers, C.L., Andrews, B., Boone, C. (2016). A global genetic interaction network maps a wiring diagram of cellular function. Science 353 (6306). pii: aaf1420.

Billmann, M., Boutros, M. (2016). Methods for High-Throughput RNAi Screening in Drosophila Cells. Methods Molecular Biology 1478:95-116.

Augustin, I., Dewi, D. L., Hundshammer, J., Rempel, E., Brunk, F., Boutros, M. (2016). Immune cell infiltration in teratomas is impaired by increased Wnt secretion. Stem Cell Research 17:607-61.

Zhan, T., Rintdorff, N., Boutros, M. (2016). Wnt signaling in cancer. Oncogene 36:1461-1473

Rauscher, B., Heigwer, F., Breinig, M., Winter, J., Boutros, M. (2017). GenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens. Nucleic Acids Research 45:D679-D686.

Augustin, I., Dewi, D.L., Hundshammer, J., Erdmann, G., Kerr, G., Boutros, M. (2017). Autocrine Wnt regulates survival and genomic stability of embryonic stem cells. Science Signaling 10:eaah6829.

Zhou, J., Edgar, B.A., Boutros, M. (2017). ATF3 acts as a rheostat to control JNK signaling during intestinal regeneration. Nature Communications 8:14289.

Billmann, M., Boutros, M. (2017). Systematic Epistatic Mapping of Cellular Processes. Cell Division 2:2. doi: 10.1186/s13008-016-0028-z.

Baliga, N.S., Björkegren, J.L., Boeke, J.D., Boutros, M., Crawford, N.P., Dudley, A.M., Farber, C.R., Jones, A., Levey, A.I., Lusis, A.J., Mak, H.C., Nadeau, J.H., Noyes, M.B., Petretto, E., Seyfried, N.T., Steinmetz, L.M., Vonesch, S.C. (2017). The State of Systems Genetics in 2017. Cell Systems 4:7-15.

Seiler, J., Breinig, M., Caudron-Herger, M., Polycarpou-Schwarz, M., Boutros, M., Diederichs, S. (2017). The lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance. Nucleic Acids Research Feb 4. doi: 10.1093/nar/gkx076.

Strassburger, K., Lorbeer, F.K., Lutz, M., Graf, F., Boutros, M., Teleman, A.A. (2017) Oxygenation and adenosine deaminase support growth and proliferation of ex vivo cultured Drosophila wing imaginal discs. Development 10.1242/dev.147538.

Worst, T.S., von Hardenberg, J., Gross, J.C., Erben, P., Schnoelzer, M., Hausser, I., Bugert, P., Michel, M.S., Boutros, M. (2017). A database-augmented, exosome-based mass spectrometry approach exemplarily identifies circulating claudin 3 as biomarker in prostate cancer. Molecular Cell Proteomics 10.1074/mcp.M117.068577.

Scheeder, C., Heigwer, F., Boutros, M. (2017). HTSvis: a web app for exploratory data analysis and visualization of arrayed high-throughput screens. Bioinformatics 10.1093/bioinformatics/btx319.

Nötzold, L., Frank, L., Gandhi, M., Polycarpou-Schwarz, M., Groß, M., Gunkel, M., Beil, N., Erfle, H., Harder, N., Rohr, K., Trendel, J., Krijgsveld, J., Longerich, T., Schirmacher, P., Boutros, M., Erhardt, S., Diederichs, S. (2017). The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells. Sci Rep. 7:2265. doi: 10.1038/s41598-017-02357-0.

 

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