The Transgenic Service provides investigators at the DKFZ with the mouse embryo manipulation technology in order to generate transgenic mice

The transgenic core services include:

  • Consultation / Support with the planning of transgenic projects
  • Microinjection of DNA into zygotes
  • CRISPR/Cas technology (endonuclease mediated genome editing)
  • Microinjection of embryonic stem cells (ES-cells) into blastocysts
  • Electroporation / Culture of embryonic stem cells
  • Gene Targeting (collaborative service)
  • Generation of ES cell lines from transgenic mice (collaborative service)
  • Morula aggregation
  • In Vitro Fertilisation
  • Cryopreservation of mouse embryos and spermatozoa
  • Lentivirus injection / incubation
  • Embryo transfer (rederivation of mouse lines)
  • Vasectomy
  • Training in transgenic techniques

Important notice

Please indicate your interest to use our core facility as early as possible, due to the necessary preparations (“Tierversuchsantrag” (license for animal experimentation), mouse ordering, reservation of housing in a mouse barrier)

to top