Cookie Settings

We use cookies to optimize our website. These include cookies that are necessary for the operation of the site, as well as those that are only used for anonymous statistic. You can decide for yourself which categories you want to allow. Further information can be found in our data privacy protection .


These cookies are necessary to run the core functionalities of this website and cannot be disabled.

Name Webedition CMS
Purpose This cookie is required by the CMS (Content Management System) Webedition for the system to function correctly. Typically, this cookie is deleted when the browser is closed.
Name econda
Purpose Session cookie emos_jcsid for the web analysis software econda. This runs in the “anonymized measurement” mode. There is no personal reference. As soon as the user leaves the site, tracking is ended and all data in the browser are automatically deleted.

These cookies help us understand how visitors interact with our website by collecting and analyzing information anonymously. Depending on the tool, one or more cookies are set by the provider.

Name econda
Purpose Statistics
External media

Content from external media platforms is blocked by default. If cookies from external media are accepted, access to this content no longer requires manual consent.

Name YouTube
Purpose Show YouTube content
Name Twitter
Purpose activate Twitter Feeds

Division of Pediatric Neurooncology

Prof. Dr. Stefan Pfister

Intratumoral heterogeneity demonstrated by amplification of distinct oncogenes (red= MYCN, green = GLI2) in different cell populations of a medulloblastoma sample.

Pediatric Neurooncology is currently a vibrant field of research. This is desperately needed, since brain tumors have become the number one cause of cancer-related mortality in children. Our group aims to bridge the gap between generating genomic screening data as well as faithful models for preclinical drug testing, and exploiting these data for the sake of our patients. The first goal includes the identification, validation and clinical application of prognostic and predictive biomarkers in different childhood brain tumors, including genome, transcriptome and epigenome analysis, and integrative bioinformatics approaches. The second major focus involves the generation of suitable in vitro and in vivo models for systematic pre-clinical testing of novel smart drugs, often in combination with established cytotoxic drugs, chemotherapy or immunotherapy. These novel therapies are ultimately translated into patient care, tightly linked with thorough patient selection based on the genetic/molecular signature of the individual tumor (“personalized cancer care”).

The thorough understanding of the immense biological heterogeneity of childhood brain tumors is a prerequisite for targeted treatment approaches. Thus, we will continue to comprehensively investigate the entire genetic and epigenetic diversity of childhood brain tumors within and across histopathological entities. Many of these novel methods are currently being prepared for routine diagnostic applications in a clinical setting through our nationally and internationally acting molecular diagnostics programs ( Preclinical models will allow us to specifically test biological hypotheses gained from genome-wide primary tumor analyses in vitro and in vivo, before they are recommended for use in patients. Another focus will be the analysis of clonality within tumors, their respective metastases, and tumor relapses, by ultra-deep next-generation and single-cell sequencing techniques. As a third major focus, we have started focusing on the detection of tumor-specific alteration in body fluids, such as cerebrospinal fluid and plasma, which can be exploited for molecular diagnostics, tumor cell clearance (minimal residual disease), detection of molecular targets, and primary resistance mechanisms. Finally, we will evaluate novel as well as many conventional therapies systematically for their inter-individual range in pharmacokinetics. These differences might help explaining successes and failures in individual patients.


Prof. Dr. Stefan Pfister
Pediatric Neurooncology (B062)
Deutsches Krebsforschungszentrum
Im Neuenheimer Feld 280
69120 Heidelberg
Tel: +49 6221 424618

Selected Publications

  • Lin, C.Y., Erkek, S. et al., …and Pfister, S.M.*, Bradner, J.E.*, Northcott, P.A.* (2016). Active medulloblastoma enhancers reveal subgroup-specific cellular origins. Nature. 530(7588):57-62.
  • Pajtler KW, Witt H, Sill M et al., …and Kool, M.*, Pfister, S.M.* (2015). Molecular Classification of Ependymal Tumors across All CNS Compartments, Histopathological Grades, and Age Groups. Cancer Cell. 27(5):728-43.
  • Northcott, P. et al., …and Peter Lichter, P.*, Korbel, J.O.*, Wechsler-Reya, R*, Pfister, S.M.* (2014) Enhancer hijacking activates GFI1 family oncogenes in medulloblastoma. Nature 511(7510):428-34.
  • Kool M et al.,…,and Wechsler-Reya, R.J.*, Lichter, P.*, Pfister, S.M*. (2014). Genome Sequencing of SHH Medulloblastoma Predicts Genotype-Related Response to Smoothened Inhibition. Cancer Cell 25(3):393-405.
to top
powered by webEdition CMS