Controls

In order to evaluate possible effects of GFP on the localization of fusion proteins we performed two types of assays.

1. We analyzed the sub-cellular localization of several fusion proteins in Vero (monkey kidney) and HeLa (human cervix carcinoma) cells. In every case the results obtained with the two cell lines have been identical, demonstrating that the sub-cellular localization in most cases will be independent on the cell line used in the analysis. Nevertheless, we will use cell lines of different origin (e.g. hepatocytes, neuronal cells) for future experiments.

2. We analyzed the sub-cellular localization of several known proteins with predicted or known localization. In most cases, the localization was verified - independent of the GFP fusion part.

We conclude that the sub-cellular localization of the proteins is mostly not affected by the GFP fusion part.

Controls

• Cell Lines
• Known Proteins