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Research

Known Proteins

Localization of N- and C-terminal fusions with known proteins

Localization of N- and C-terminal fusions with known proteins

A selection of known characterised proteins representing various subcellular compartments were taken, amplified and cloned into the modified CFP and YFP expression vectors via the Gateway cloning system. These were then transfected into Vero cells and the localisations of the fusion proteins recorded. In most cases the expected localisations (marked in green) were indeed observed. Only in two of the examples tested were the observed localisations incorrect (marked in red). In general the GFP tagging strategy using Gateway does not appear to inhibit the correct localisation of expressed proteins.