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Recombinant antibodies

For recombinant antibody production in HEK cells, RNA of hybridomas is sequenced, the variable immunoglobulin regions identified, cloned in frame into different Fc backbones and the immunoglobulin chains are expressed.

  • RNA isolation and RNA sequencing of stable hybridoma cell line
  • Identification of variable regions of the heavy and light chain
  • Cloning into Fc backbone (human, mouse, rabbit, Fab with HIS- or Streptavidin-tag), scFv
  • Antibody production through transient expression in HEK cells



     

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last update:
23/01/2025
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