Monoclonal Antibodies
The Core Facility Antibodies uses state-of-the-art hybridoma technologies for production of monoclonal antibodies via the somatic fusion of lymphocytes from immunized mice or rats. We advise in the selection of antigens, immunize animals and perform screening of mother clones by e.g. ELISA, immunofluorescence or IHC. Clones having been tested successfully will be subcloned, expanded and maintained to guarantee long-time availability of materials. In addition, we provide non-GMP production and purification of antibodies.
Our service includes:
- Advice in the selection of the antigen (protein/peptide)
- Alignment analysis to check for cross-reactivity
- Immunization of animals
- Titer test
- Advice in validation of antibody specificity
- Consultation and close interaction with customers during all steps of production
Our workflow:
- Fusion of lymphocytes to immortalized cell line
- Screening of clones by ELISA/Western blot/Immunofluorescence
- Selection of clones displaying desired specificity
- Sub-cloning to capture stable cell lines
- Estimation of antibody subgroup
- Expansion and storage of positive clones
- Supply with supernatant for your experiments
- If required, scale up and antibody purification
last update:
23/01/2025