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Practical Course HP-F12: Immunological Methods

Type: Practical Course with Student Seminars

Date: 17. June - 5. July 2024



Christian Busse, Stefan Eichmüller, Viktor Umansky (responsible organizer contacts:,,
Branko Cirovic, Nikolaus Dietlein, Christine Engeland, Alessia Floerchinger, Thorsten Feyerabend, Ed Green, Felix Hartmann, Johannes Heidbüchel, Wolfram Osen, Thomas Plum, Philipp Rößner, Martina Seiffert


Methods in experimental immunology and tumor immunology including cellular analysis, single-cell assays, sequencing, and various functional assays. The practical covers a selection of these methods routinely applied by research groups working in the tumor immunology field.

Contents (practical part):

  • Introduction into flow cytometry and cell sorting
  • Mulitplexed Ion Beam Imaging
  • In-vitro differentiation of HSPCs
  • Lymphoma – Treatment (Isolation of peripheral blood mononuclear cells (PBMC) and immune cells from blood; In vitro drug testing using primary cancer and immune cells; Multi-color flow cytometry to determine apoptosis and T cell activation; Analysis of FACS data and interpretation of results)
  • Basic immune repertoire analysis using R
  • T cells – Bi-specific T-cell engagers (BiTEs) (Detection of antigen-specific T cells using FACS-based cytokine secretion assay; Functional T cell assays: ICS, ELISPOT, ELISA)
  • T cells – Anti-tumor responses
  • Quantification of the type 1 hyper-reactive mast cell degranulation

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