Practical Course HP-F12: Immunological Methods
Type: Practical Course with Student Seminars
Date: 13. June -11. July 2022
Hosts/Supervisors:
Christian Busse, Stefan Eichmüller, Viktor Umansky (responsible organizer contacts: christian.busse@dkfz.de, s.eichmueller@dkfz.de, v.umansky@dkfz.de)
Branko Cirovic, Nikolaus Dietlein, Christine Engeland, Thorsten Feyerabend, Ed Green, Felix Hartmann, Johannes Heidbüchel, Wolfram Osen, Thomas Plum, Sandrine Sander, Martina Seiffert
Topics:
Methods in experimental immunology and tumor immunology including cellular analysis, single-cell assays, sequencing, and various functional assays. The practical course covers a selection of these methods routinely applied by research groups working in the tumor immunology field.
Contents (practical part):
- Introduction into flow cytometry and cell sorting
- B cell development and germinal center structure
- Sequence and repertoire analysis of immunoglobulins
- Affinity maturation of antibodies
- Quantification of the type 1 hyper-reactive mast cell degranulation
- T cell metabolism
- Bi-specific T-cell engagers (BiTEs)
- Detection of immune cell infiltration in tumors using immunofluorescence techniques
- Detection of antigen-specific T cells using FACS-based cytokine secretion assay
- Functional T cell assays: ICS, ELISPOT, ELISA
- Multiplexed Ion Beam Imaging
- Lymphoma – Treatment
- Basic immune repertoire analysis using R