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Practical Course HP-F12: Immunological Methods

Type: Practical Course with Student Seminars

Date: 17. June - 5. July 2024

 

Hosts/Supervisors:

Christian Busse, Stefan Eichmüller, Viktor Umansky (responsible organizer contacts: christian.busse@dkfz.de, s.eichmueller@dkfz.de, v.umansky@dkfz.de)
Branko Cirovic, Nikolaus Dietlein, Christine Engeland, Alessia Floerchinger, Thorsten Feyerabend, Ed Green, Felix Hartmann, Johannes Heidbüchel, Wolfram Osen, Thomas Plum, Philipp Rößner, Martina Seiffert

Topics:

Methods in experimental immunology and tumor immunology including cellular analysis, single-cell assays, sequencing, and various functional assays. The practical covers a selection of these methods routinely applied by research groups working in the tumor immunology field.

Contents (practical part):

  • Introduction into flow cytometry and cell sorting
  • Mulitplexed Ion Beam Imaging
  • In-vitro differentiation of HSPCs
  • Lymphoma – Treatment (Isolation of peripheral blood mononuclear cells (PBMC) and immune cells from blood; In vitro drug testing using primary cancer and immune cells; Multi-color flow cytometry to determine apoptosis and T cell activation; Analysis of FACS data and interpretation of results)
  • Basic immune repertoire analysis using R
  • T cells – Bi-specific T-cell engagers (BiTEs) (Detection of antigen-specific T cells using FACS-based cytokine secretion assay; Functional T cell assays: ICS, ELISPOT, ELISA)
  • T cells – Anti-tumor responses
  • Quantification of the type 1 hyper-reactive mast cell degranulation

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