Practical Course HP-F12: Immunological Methods
Type: Practical Course with Student Seminars
Date: 15. June - 7. July 2023
Hosts/Supervisors:
Christian Busse, Stefan Eichmüller, Viktor Umansky (responsible organizer contacts: christian.busse@dkfz.de, s.eichmueller@dkfz.de, v.umansky@dkfz.de)
Branko Cirovic, Nikolaus Dietlein, Christine Engeland, Alessia Floerchinger, Thorsten Feyerabend, Ed Green, Felix Hartmann, Johannes Heidbüchel, Wolfram Osen, Thomas Plum, Philipp Rößner, Martina Seiffert
Topics:
Methods in experimental immunology and tumor immunology including cellular analysis, single-cell assays, sequencing, and various functional assays. The practical covers a selection of these methods routinely applied by research groups working in the tumor immunology field.
Contents (practical part):
- Basic immune repertoire analysis using R
- Introduction into flow cytometry and cell sorting
- Mulitplexed Ion Beam Imaging
- In-vitro differentiation of HSPCs
- Lymphoma – Treatment (Isolation of peripheral blood mononuclear cells (PBMC) and immune cells from blood; In vitro drug testing using primary cancer and immune cells; Multi-color flow cytometry to determine apoptosis and T cell activation; Analysis of FACS data and interpretation of results)
- T cells – Bi-specific T-cell engagers (BiTEs) (Detection of antigen-specific T cells using FACS-based cytokine secretion assay; Functional T cell assays: ICS, ELISPOT, ELISA)
- Quantification of the type 1 hyper-reactive mast cell degranulation