Five selected Publications

Protein quality control at the inner nuclear membrane. Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus DJ, Le Dez G, Brossard A, Gunnarsson A, Barry JD, Meurer M, Kirrmaier D, Boone C, Huber W, Rabut G, Ljungdahl PO, Knop M. Nature. 2014 Dec 18;516(7531):410-3. doi: 10.1038/nature14096.

Directional tissue migration through a self-generated chemokine gradient. Donà E, Barry JD, Valentin G, Quirin C, Khmelinskii A, Kunze A, Durdu S, Newton LR, Fernandez-Minan A, Huber W, Knop M, Gilmour D. Nature. 2013 Nov 14;503(7475):285-9. doi: 10.1038/nature12635. Epub 2013 Sep 25.

Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy. Gibeaux R, Politi AZ, Nédélec F, Antony C, Knop M. Genes Dev. 2013 Feb 1;27(3):335-49. doi: 10.1101/gad.206318.112.

Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E, Knop M. Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281.

Segregation of yeast nuclear pores. Khmelinskii A, Keller PJ, Lorenz H, Schiebel E, Knop M. Nature. 2010 Jul 22;466(7305):E1. doi: 10.1038/nature09255.

Publications

Protein quality control at the inner nuclear membrane.
 Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus DJ, Le Dez G, Brossard A, Gunnarsson A, Barry JD, Meurer M, Kirrmaier D, Boone C, Huber W, Rabut G, Ljungdahl PO, Knop M.
 Nature. 2014 Dec 18;516(7531):410-3. doi: 10.1038/nature14096.

PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast.
 Huber F, Meurer M, Bunina D, Kats I, Maeder CI, Stefl M, Mongis C, Knop M.
 PLoS One. 2014 Dec 10;9(12):e114590. doi: 10.1371/journal.pone.0114590. eCollection 2014.

A memory system of negative polarity cues prevents replicative aging.
 Meitinger F, Khmelinskii A, Morlot S, Kurtulmus B, Palani S, Andres-Pons A, Hub B, Knop M, Charvin G, Pereira G.
 Cell. 2014 Nov 20;159(5):1056-69. doi: 10.1016/j.cell.2014.10.014. Epub 2014 Nov 13.

Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis.
 Boeke D, Trautmann S, Meurer M, Wachsmuth M, Godlee C, Knop M, Kaksonen M.
 Mol Syst Biol. 2014 Nov 3;10:756. doi: 10.15252/msb.20145422.

Analytical model for macromolecular partitioning during yeast cell division
 Kinkhabwala, A, Khmelinskii, A, and Knop, M. BMC Biophysics 2014, 7:10

PSFj: know your fluorescence microscope.
 Theer P, Mongis C, Knop M.
 Nat Methods. 2014 Oct;11(10):981-2. doi: 10.1038/nmeth.3102. No abstract available.

Analysis of protein dynamics with tandem fluorescent protein timers.
 Khmelinskii A, Knop M.
 Methods Mol Biol. 2014;1174:195-210. doi: 10.1007/978-1-4939-0944-5_13.

Tracking protein turnover and degradation by microscopy: photo-switchable versus time-encoded fluorescent proteins.
 Knop M, Edgar BA.
 Open Biol. 2014 Apr 16;4:140002. doi: 10.1098/rsob.140002.

Determinants of robustness in spindle assembly checkpoint signalling
 Heinrich, S, Geissen, EM, Kamenz, J, Trautmann, S, Widmer, C, Drewe, P, Knop, M, Radde, N, Hasenauer, J, Hauf, S
 Nat Cell Biol. 2013 Nov;15(11):1328-39. doi: 10.1038/ncb2864. Epub 2013 Oct 27.

Directional tissue migration through a self-generated chemokine gradient.
Donà E, Barry JD, Valentin G, Quirin C, Khmelinskii A, Kunze A, Durdu S, Newton LR, Fernandez-Minan A, Huber W, Knop M, Gilmour D.
 Nature. 2013 Nov 14;503(7475):285-9. doi: 10.1038/nature12635. Epub 2013 Sep 25.

When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved.
 Gibeaux R, Knop M.
 Nucleus. 2013 May-Jun;4(3):182-8. doi: 10.4161/nucl.25021. Epub 2013 May 15. Review.

Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.
 Gibeaux R, Politi AZ, Nédélec F, Antony C, Knop M.
 Genes Dev. 2013 Feb 1;27(3):335-49. doi: 10.1101/gad.206318.112.

Tandem fluorescent protein timers for in vivo analysis of protein dynamics.
 Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E, Knop M.
 Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281.

TIPI: TEV protease-mediated induction of protein instability.
 Taxis C, Knop M.
 Methods Mol Biol. 2012;832:611-26. doi: 10.1007/978-1-61779-474-2_43.

Seamless gene tagging by endonuclease-driven homologous recombination.
 Khmelinskii A, Meurer M, Duishoev N, Delhomme N, Knop M.
 PLoS One. 2011;6(8):e23794. doi: 10.1371/journal.pone.0023794. Epub 2011 Aug 22.

Exploiting spore-autonomous fluorescent protein expression to quantify meiotic chromosome behaviors in Saccharomyces cerevisiae.
 Thacker D, Lam I, Knop M, Keeney S.
 Genetics. 2011 Oct;189(2):423-39. doi: 10.1534/genetics.111.131326. Epub 2011 Aug 11.

Yeast cell morphology and sexual reproduction--a short overview and some considerations.
 Knop M.
 C R Biol. 2011 Aug-Sep;334(8-9):599-606. doi: 10.1016/j.crvi.2011.05.007. Epub 2011 Jul 1. Review.

Genomic promoter replacement cassettes to alter gene expression in the yeast Saccharomyces cerevisiae.
 Kaufmann A, Knop M.
 Methods Mol Biol. 2011;765:275-94. doi: 10.1007/978-1-61779-197-0_16.

Artificial tethering to nuclear pores promotes partitioning of extrachromosomal DNA during yeast asymmetric cell division.
 Khmelinskii A, Meurer M, Knop M, Schiebel E.
 Curr Biol. 2011 Jan 11;21(1):R17-8. doi: 10.1016/j.cub.2010.11.034.

Segregation of yeast nuclear pores.
 Khmelinskii A, Keller PJ, Lorenz H, Schiebel E, Knop M.
 Nature. 2010 Jul 22;466(7305):E1. doi: 10.1038/nature09255.

Evolution of mutational robustness in the yeast genome: a link to essential genes and meiotic recombination hotspots.
 Keller PJ, Knop M.
 PLoS Genet. 2009 Jun;5(6):e1000533. doi: 10.1371/journal.pgen.1000533. Epub 2009 Jun 26.

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron.
 Taxis C, Stier G, Spadaccini R, Knop M.
 Mol Syst Biol. 2009;5:267. doi: 10.1038/msb.2009.25. Epub 2009 Apr 28.

The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis.
 Maier P, Rathfelder N, Maeder CI, Colombelli J, Stelzer EH, Knop M.
 EMBO J. 2008 Sep 17;27(18):2363-74. doi: 10.1038/emboj.2008.168. Epub 2008 Aug 28.

Reconstitution of Mdm2-dependent post-translational modifications of p53 in yeast.
 Di Ventura B, Funaya C, Antony C, Knop M, Serrano L.
 PLoS One. 2008 Jan 30;3(1):e1507. doi: 10.1371/journal.pone.0001507.

Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling.
 Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PI, Knop M.
 Nat Cell Biol. 2007 Nov;9(11):1319-26. Epub 2007 Oct 21.

Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane.
 Maier P, Rathfelder N, Finkbeiner MG, Taxis C, Mazza M, Le Panse S, Haguenauer-Tsapis R, Knop M.
 EMBO J. 2007 Apr 4;26(7):1843-52. Epub 2007 Mar 8.

Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast.
 Taxis C, Maeder C, Reber S, Rathfelder N, Miura K, Greger K, Stelzer EH, Knop M.
 Traffic. 2006 Dec;7(12):1628-42.

Evolution of the hemiascomycete yeasts: on life styles and the importance of inbreeding.
 Knop M.
 Bioessays. 2006 Jul;28(7):696-708. Review.

Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis.
 Gordon O, Taxis C, Keller PJ, Benjak A, Stelzer EH, Simchen G, Knop M.
 EMBO J. 2006 Aug 23;25(16):3856-68. Epub 2006 Aug 3.

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae.
 Taxis C, Knop M.
 Biotechniques. 2006 Jan;40(1):73-8.

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system.
 Taxis C, Keller P, Kavagiou Z, Jensen LJ, Colombelli J, Bork P, Stelzer EH, Knop M.
 J Cell Biol. 2005 Nov 21;171(4):627-40. Epub 2005 Nov 14.

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization.
 Reynaud EG, Andrade MA, Bonneau F, Ly TB, Knop M, Scheffzek K, Pepperkok R.
 BMC Biol. 2005 Oct 7;3:21.

Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis.
 Riedel CG, Mazza M, Maier P, Körner R, Knop M.
 J Biol Chem. 2005 Nov 11;280(45):37846-52. Epub 2005 Sep 7.

Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast.
 Knop M, Miller KJ, Mazza M, Feng D, Weber M, Keränen S, Jäntti J.
 Mol Biol Cell. 2005 Oct;16(10):4543-56. Epub 2005 Jul 19.

A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
 Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.
 Yeast. 2004 Aug;21(11):947-62.

Regulation of exocytotic events by centrosome-analogous structures. Taxis, C. & Knop, M. Topics Curr. Gen. 2004 10 193-207

Prospore membrane formation: how budding yeast gets shaped in meiosis.
 Moreno-Borchart AC, Knop M.
 Microbiol Res. 2003;158(2):83-90. Review.

Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.
 Maekawa H, Usui T, Knop M, Schiebel E.
 EMBO J. 2003 Feb 3;22(3):438-49.

 Improved version of the red fluorescent protein (drFP583/DsRed/RFP).
 Knop M, Barr F, Riedel CG, Heckel T, Reichel C.
 Biotechniques. 2002 Sep;33(3):592, 594, 596-8 passim.

Prospore membrane formation linked to the leading edge protein (LEP) coat assembly.
 Moreno-Borchart AC, Strasser K, Finkbeiner MG, Shevchenko A, Shevchenko A, Knop M.
 EMBO J. 2001 Dec 17;20(24):6946-57.

A screen for genes required for meiosis and spore formation based on whole-genome expression.
 Rabitsch KP, Tóth A, Gálová M, Schleiffer A, Schaffner G, Aigner E, Rupp C, Penkner AM, Moreno-Borchart AC, Primig M, Esposito RE, Klein F, Knop M, Nasmyth K.
 Curr Biol. 2001 Jul 10;11(13):1001-9.

Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis.
 Knop M, Strasser K.
 EMBO J. 2000 Jul 17;19(14):3657-67.

Microtubule organization by the budding yeast spindle pole body.
 Knop M, Pereira G, Schiebel E.
 Biol Cell. 1999 May-Jun;91(4-5):291-304. Review.

Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy.
 Pereira G, Grueneberg U, Knop M, Schiebel E.
 EMBO J. 1999 Aug 2;18(15):4180-95.

Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines.
 Knop M, Siegers K, Pereira G, Zachariae W, Winsor B, Nasmyth K, Schiebel E.
 Yeast. 1999 Jul;15(10B):963-72.

Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication.
 Elliott S, Knop M, Schlenstedt G, Schiebel E.
 Proc Natl Acad Sci U S A. 1999 May 25;96(11):6205-10.

Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation.
 Knop M, Schiebel E.
 EMBO J. 1998 Jul 15;17(14):3952-67.

Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body.
 Pereira G, Knop M, Schiebel E.
 Mol Biol Cell. 1998 Apr;9(4):775-93.

Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p.
 Knop M, Schiebel E.
 EMBO J. 1997 Dec 1;16(23):6985-95.

The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication.
 Knop M, Pereira G, Geissler S, Grein K, Schiebel E.
 EMBO J. 1997 Apr 1;16(7):1550-64.

N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast.
 Knop M, Hauser N, Wolf DH.
 Yeast. 1996 Sep 30;12(12):1229-38.

The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment.
 Geissler S, Pereira G, Spang A, Knop M, Souès S, Kilmartin J, Schiebel E.
 EMBO J. 1996 Aug 1;15(15):3899-911. Erratum in: EMBO J 1996 Sep 16;15(18):5124.

Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast.
 Knop M, Finger A, Braun T, Hellmuth K, Wolf DH.
 EMBO J. 1996 Feb 15;15(4):753-63.

Analysis of two mutated vacuolar proteins reveals a degradation pathway in the endoplasmic reticulum or a related compartment of yeast.
 Finger A, Knop M, Wolf DH.
 Eur J Biochem. 1993 Dec 1;218(2):565-74.

Vacuolar/lysosomal proteolysis: proteases, substrates, mechanisms.
 Knop M, Schiffer HH, Rupp S, Wolf DH.
 Curr Opin Cell Biol. 1993 Dec;5(6):990-6. Review.

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