Dr. Steffen Schmitt

© dkfz.de

Flow Cytometry is a powerful analysis tool in modern biology and life sciences. The applications are numerous and have contributed to many fields including virology, immunology, and molecular biology. The major advantages include the analysis of high cell numbers per sample combined with an increasing number of parameters. Based on the expression pattern of those parameters, it is possible to isolate specific cell populations for further analysis (e.g. gene expression pattern, functional assays, etc.).

Single cells stained with fluorescent dye coupled monoclonal antibodies (or other bioreactive molecules) emit characteristic light signals, while passing one or more focused laser beams. These events can then be collected and analysed (with a speed of 10.000 - 30.000 particles/ sec.). At the moment we can analyse with our instruments up to 19 parameters at once while collecting 4 different subpopulations for further analysis.

The core facility flow cytometry is equipped with 7 instruments for analysing, 4 cell sorters and various software modules to process and toanalysethe samples. Upon request, we will help you plan, perform and interpret your FACS® (fluorescence activated cell sorting) and cell sorting experiments. The Core Facility Flow Cytometry is also involved in training and education of PhD-students and staffmembers of the DKFZ (see program Aus- und Weiterbildung). If you want to use our services, you have to provide us with a signed hard copy of our user guidelines. After training the analysers can be used by the users itself. The cell sorters are exclusively operated by the facility staff. Appointments could be made directly with the responsible operator via sending an e-mail (see below).

DKFZ members and cooperation partners will find further informations about our support and facility equipment on our intranet pages (see below).

Please don’t hesitate to contact us, if you have further questions:

Dr. Steffen Schmitt
Phone: 42-1261 (office)

Klaus Hexel
Phone: 42-3641

Gelo dela Cruz
Phone: 42-3643

Jens Hartwig
Phone: 42-3642

Fax: 42-3755
E-Mail: cytometry@dkfz.de
Intranet: Scientific Services - Core Facilities