Head: Prof. Dr. Holger Sültmann

Senior Scientists and Postdocs: Prof. Dr. Peter Altevogt, PD Dr. Sabine Klauck, Dr. Doreen Heckmann-Nötzel

Computational Scientists: Dr. Vladimir Kuryshev, Clémentine Mercé

PhD students: Leonie Ratz, Steffen Dietz, Rebecca Weber (with Thoraxklinik HD), Arlou Kristina Angeles, Sebastian Weingart, Simone Bauer, Ann-Kathrin Daum

MD students: Anja Riediger, Sabrina Müller

MSc student: Florian Janke

BSc student: Niclas Flosdorf

Technical assistants: Sabrina Gerhardt, Simon Ogrodnik

 

Contact:
Prof. Dr. rer. nat. Holger Sültmann
DKFZ and NCT Heidelberg
Im Neuenheimer Feld 460
69120 Heidelberg, Germany
Tel.: ++49 6221 565934
Fax: ++49 6221 565382
E-Mail: h.sueltmann@dkfz.de
(Directions)


Cancer cells are genetically different from normal cells and often exhibit aberrant genome structures and deregulated gene expression. The characterization of the global gene expression changes typical for certain tumor types and stages comprises potential molecular markers for diagnosis and prognosis and can also provide insights into the mechanisms of tumor progression.

(Epi)genomics and transcriptomics of cancer

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In the past, we have successfully established and coordinated interdisciplinary cancer research projects in (NGFNplus and ICGC), and analyzed multiple orthogonal data sets on the same tumor samples. The initial step in many of our projects consists of applying genomics and epigenomics technologies to identify molecular signatures and genes for tumor stratification. Technology-wise, our focus is on the application and analysis of ultra-high throughput DNA and RNA-Seq and microarray data for DNA mutations as well as mRNA and miRNA expression. We are also increasingly analyzing the contribution of epigenomic alterations (DNA methylation, histone modification) to the regulation of mRNAs and miRNAs involved in malignant processes and the occurence of therapy resistance.

Cellular analysis of gene and protein function

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To characterize the functions of the genes emerging from our screening studies, we apply cellular functional assays in the individual tumor backgrounds to overexpress or silence (RNAi, CRISPR/Cas9) selected mRNAs or miRNAs and to investigate their impact on tumorigenic processes. In addition, we use quantitative proteomics (Bioplex) to investigate signaling pathway activities. We also exploit established high throughput technologies (e.g. CRISPR/Cas9 screens) to search for synthetic lethal interactions in defined genetic tumor cell backgrounds.

Molecular tumor stratification and translation

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In order to advance our research results into clinically useful applications, we have identified DNA, mRNA, and miRNA signatures in tissues for the diagnosis and prognosis of cancer and successfully implemented technologies (exome seq and digital PCR) to quantify circulating nucleic acids (DNA mutations, miRNA abundance) in blood plasma (“liquid biopsy”) for tumor detection, prognosis, and monitoring. Our aim is to translate these markers into the clinical setting and to embed the identification of biomarkers as research efforts into investigator-initiated clinical studies (DKTK, DZL, HIPO).

Computational biology

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Data analyses and network modeling approaches are performed by computational scientists in close collaboration with the Division of Theoretical Bioinformatics at DKFZ.



Former group members:
Dr. Julia Romic-Pickl, Dr. Sajo Kaduthanam, Dr. Uwe Schirmer, Denise Keitel, Dr. Ruprecht Kuner, Dr. Helen Hülsmann, Dr. Holger Armbruster, Dr. Mark Laible, Dr. Daniela Wuttig, Dr. Julia Starmann, Jennifer Metzger, Marina Gonzalez, Dr. Lukasz Kacprzyk, Dr. Stephan Gade, Sara Myers, Dr. Maria Fälth, Tatjana Andrasiuk, Dr. Rainer Will, Dr. Marc Johannes, Dr. Jan Christoph Brase, Dr. Stephanie Wittig-Blaich, Dr. Melanie Bewerunge-Hudler, Rita Schatten, Angelika Wörner, Thorsten Kühlwein, Dr. Nicole Chui Pressinotti, Dr. Caroline End, Prof. Dr. Tim Beißbarth, Dr. Alberto Calabró, Dr. Mark Fellmann, Dr. Christian Löbke, Dr. Markus Ruschhaupt, Dr. Van Duc Luu, Dr. Michael Stojanov, Andreas Buneß, Dr. Claudia Heine, Prof. Dr. Holger Fröhlich, Prof. Dr. Achim Tresch, Dr. Jörg Schneider, Dr. Katharina Finis, Dr. Wolfgang Huber, Dr. Markus Vogt, Prof. Dr. Florian Haller, Gabi Rottsahl, Claudia Grosser


Support:

German Cancer Consortium (DKTK)

German Center for Lung Research (DZL)

German Federal Ministry for Science and Education (BMBF).

Bruno und Helene Jöster Stiftung

Deutsche Krebshilfe

Heidelberg Institute for Personalized Oncology (HIPO)

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