Selected Publications

  • Boutros M., Paricio, N., Strutt, D., Mlodzik, M. (1998). Dishevelled activates JNK
    and discriminates between JNK pathways in planar polarity and wingless signaling.
    Cell 94:109-18.
  • Boutros, M., Mihaly, J., Bouwmeester, T., Mlodzik, M. (2000). Signaling specificity by
    Frizzled receptors in Drosophila. Science 288:1825-8.
  • Boutros, M., Kiger, A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S., HD
    FlyArray, Paro, R., Perrimon, N. (2004). Genome-wide RNAi Analysis of Cell Growth
    and Viability in Drosophila. Science 303:832-835.
  • Bartscherer, K., Pelte, N., Ingelfinger, D., Boutros, M. (2006). Secretion of Wnt ligands
    requires Evi, a conserved transmembrane protein. Cell 125:523-33.
  • Boutros, M., L. Bras, W. Huber. (2006). Analysis of cell-based RNAi screens.
    Genome Biology 7:R66
  • Boutros M., Ahringer, J. (2008). The art and design of genetic screens: RNA
    interference. Nature Reviews Genetics 9:554-566.
  • Horn, T., Sandmann, T., Fischer, B., Axelsson, E., Huber, W., Boutros, M. (2011).
    Mapping of signalling networks through synthetic genetic interaction analysis by RNAi.
    Nature Methods 8:341-6.
  • Gross, J., Chaudhary, V., Bartscherer, K., Boutros, M. (2012). Active Wnt proteins are
    secreted on exosomes. Nature Cell Biology 14:1036-45.
  • Laufer, C., Fischer, B., Billmann, M., Huber, W., Boutros, M. (2013). Mapping genetic
    interactions in human cancer cells with RNAi and multiparametric phenotyping.
    Nature Methods
    10:427–431.
  • Voloshanenko, O., Erdmann, G., Dubash, T.D., Augustin, I., Metzig, M., Moffa, G.,
    Hundsrucker, C., Kerr, G., Sandmann, T., Anchang, B., Demir, K., Boehm, C., Leible,
    S., Ball, C.R., Glimm, H., Spang, R., Boutros, M. (2013). Wnt secretion is required to
    maintain high levels of Wnt activity in colon cancer cells.
    Nature Communications 4:2610.
  • Fischer, B., Sandmann, T., Horn, T., Billmann, M., Chaudhary, V., Huber, W., Boutros,
    M (2015). A Map of Directional Genetic Interactions in a Metazoan Cell.
    eLife 4 doi:10.7554/eLife.05464.

Complete List of Publications

  • Chong, J.A., Tapia-Ramirez, J., Kim, S., Toledo-Aral, J.J., Zheng, Y.,
    Boutros, M.C., Altshuller, Y.M., Frohman, M.A., Kraner, S.D., Mandel, G.
    (1995). REST: A Mammalian Silencer Protein That Restricts Sodium
    Channel Gene Expression to Neurons. Cell 80:949-57.
  • Boutros M., Paricio, N., Strutt, D., Mlodzik, M. (1998). Dishevelled activates
    JNK and discriminates between JNK pathways in planar polarity and
    wingless signaling. Cell 94:109-18.
  • Paricio N., Feiguin, F., Boutros, M., Eaton, S., Mlodzik, M. (1999). The
    Drosophila STE20-like kinase misshapen is required downstream of the
    Frizzled receptor in planar polarity signaling. EMBO Journal 18:4669-78.
  • Boutros M., Mlodzik, M. (1999). Dishevelled: at the crossroads of divergent
    intracellular signaling pathways. Mechanisms of Development 83:27-37.
  • Wilson, A.C., Boutros, M., Johnson, K.M., Herr, W. (2000). HCF Amino- and
    Carboxy-Terminal Subunit Association through Two Separate Sets of
    Interaction Modules: Involvement of Fibronectin Type 3 Repeats. Molecular
    and Cellular Biology 20:6721-30.
  • Boutros M., Perrimon, N. (2000). Drosophila genome takes flight.
    Nature Cell Biology 2:E53-4.
  • Boutros, M., Mihaly, J., Bouwmeester, T., Mlodzik, M. (2000). Signaling
    specificity by Frizzled receptors in Drosophila. Science 288:1825-8.
  • Moon R.T., Bowerman, B., Boutros, M., Perrimon, N. (2002). The promise
    and perils of Wnt signaling through beta-catenin. Science 296:1644-6.
  • Boutros, M., Agaisse, H., Perrimon, N. (2002). Sequential activation of
    signaling pathways during innate immune responses in Drosophila.
    Developmental Cell 3:711-22.  
  • Agaisse, H., Petersen, U.-M., Boutros, M., Mathey-Prevot, B., Perrimon, N.
    (2003). Signaling Role of Hemocytes in Drosophila JAK/STAT-Dependent
    Response to Septic Injury. Developmental Cell 5:441-50.
  • Hild, M., Beckmann, B., Haas, S., Koch, B., Solovyev, V., Busold, C.,
    Fellenberg, K., Boutros, M., Vingron, M., Sauer, F., Hoheisel, J. D., Paro, R.
    (2003). An integrated gene annotation and transcriptional profiling
    approach towards the full gene content of the Drosophila genome.
    Genome Biology 5:R3.
  • Boutros, M., Kiger, A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S.,
    HD FlyArray, Paro, R., Perrimon, N. (2004). Genome-wide RNAi Analysis of
    Cell Growth and Viability in Drosophila. Science 303:832-835.
  • Gesellchen V., Boutros, M. (2004). Managing the genome: microRNAs in
    Drosophila. Differentiation 72:74-80.
  • Kuttenkeuler D., Boutros, M. (2004). Genome-wide RNAi as a route to gene
    function in Drosophila. Brief Funct Genomic Proteomic 3:168-76.
  • Choe, S. E., Boutros, M., Michelson, A.M., Church, G.M., Halfon, M.S.
    (2005). Preferred analysis methods for Affymetrix GeneChips revealed by a
    wholly-defined control dataset. Genome Biology 6:R16.
  • Armknecht, S., Boutros, M., Kiger, A., Nybakken, K., Mathey-Prevot, B.,
    Perrimon, N.. (2005). High-throughput RNA interference screens in
    Drosophila tissue culture cells. Methods Enzymology 392:55-73.
  • Arziman, Z., Horn, T., Boutros, M. (2005). E-RNAi: A web application to
    design optimized RNAi constructs. Nucleic Acids Research 33:W582-88.
  • Muller, P., Kuttenkeuler, D., Gesellchen, V., Zeidler, M., Boutros, M. (2005).
    Identification of JAK/STAT signaling components by genome-wide RNAi.
    Nature 436:871-5.  
  • Gesellchen, V., Kuttenkeuler, D., Steckel, M., Pelte, N., Boutros, M. (2005).
    An RNAi screen identified a Role for IAP2 in Innate Immune Response in
    Drosophila. EMBO reports 6:979-984.
  • Fuchs, F., Boutros, M. (2006). Cellular phenotyping by RNAi. Brief Funct
    Genomic Proteomic 5:52-6.
  • Dasgupta, R., Boutros, M., Perrimon, N. (2005) Drosophila Wnt/Fz
    pathways. Sci STKE 2005:cm5.
  • Flockhart, I., Booker, M., Kiger, A., Boutros, M., Armknecht, S., Ramadan,
    N., Richardson, K., Xu, A., Perrimon, N., Mathey-Prevot, B. (2006).
    FlyRNAi: the Drosophila RNAi screening center database.
    Nucleic Acids Research 34:D489–94.
  • Bartscherer, K., Pelte, N., Ingelfinger, D., Boutros, M. (2006). Secretion of
    Wnt ligands requires Evi, a conserved transmembrane protein.
    Cell 125:523-33.
  • Echeverri C.J., Beachy. P.A., Baum, B., Boutros, M., Buchholz, F., Chanda,
    S.K., Downward, J., Ellenberg, J., Fraser, A.G., Hacohen, N., Hahn, W.C.,
    Jackson, A.L., Kiger, A., Linsley, P.S., Lum, L., Ma, Y., Mathey-Prevot, B.,
    Root, D.E., Sabatini, D.M., Taipale, J., Perrimon, N., Bernards, R.  (2006).
    Minimizing the risk of reporting false positives in large-scale RNAi screens.
    Nature Methods 3:777-9.
  • Boutros, M., L. Bras, W. Huber. (2006). Analysis of cell-based RNAi screens.
    Genome Biology 7:R66
  • Horn, T., Arziman, Z., Berger, J., Boutros, M. (2007). GenomeRNAi: a
    database for cell-based RNAi phenotypes. Nucleic Acid Research 35:D492-7
  • Eulalio, A., Rehwinkel, J., Stricker, M., Huntzinger, E., Yang, S.F., Doerks,
    T., Dorner, S., Bork, P., Boutros, M., Izaurralde, E. (2007). Target-specific
    requirements for enhancers of decapping in miRNA-mediated gene
    silencing. Genes and Development 21:2558-2570.
  • Goto, A., Matsushita, K., Gesellchen, V., El Chamy L., Kuttenkeuler, D.,
    Takeuchi, O., Hoffmann, J.A., Akira, S., Boutros, M., Reichhart, J.M. (2008).
    Akirins are highly conserved nuclear proteins required for NF-kappaB-
    dependent gene expression in Drosophila and mice. Nature Immunology
    9:97-10.
  • Stielow, B., Sapetschig, A., Krueger, I., Kunert, N., Brehm, A., Boutros, M.,
    Suske, G. (2008). Identification of SUMO-dependent chromatin-associated
    transcriptional repression components by a genome-wide RNAi screen.
    Molecular Cell 29:742-754.
  • Boutros M., Ahringer, J. (2008). The art and design of genetic screens: RNA
    interference. Nature Reviews Genetics 9:554-566.
  • Mueller P., Boutros, M., Zeidler, M. (2008). Identification of JAK/STAT
    pathway regulators-Insights from RNAi screens. Seminar Cell Dev Biol 19:360-369.
  • Steinbrink, S., Boutros, M. (2008). RNAi screening in cultured Drosophila
    cells. Methods Molecular Biology 420:139-1353.
  • Bartscherer, K., Boutros, M. (2008). Regulation of Wnt protein secretion
    and its role in gradient formation. EMBO reports 9:977-982.
  • Adell, T., Salo, E., Boutros, M., Bartscherer, K. (2009). Smed-Evi/Wntless is
    required for b-catenin-dependent and –independent processes during
    planarian regeneration. Development 136:905-910.
  • Gauhar, Z., Sun, L.V., Hua, S., Mason, C.E., Fuchs, F., Li, T.R., Boutros, M.,
    White, K.P. (2009). Genomic mapping of binding regions for the ecdysone
    receptor protein complex. Genome Research 19:1006-1013.
  • Simons, M., Gault, W.J., Gotthardt, D., Rohatgi, R., Klein, T.J., Shao, Y.,
    Lee, H.J., Wu, A.L., Fang, Y., Satlin, L.M., Dow, J.T., Chen, J., Zheng, J.,
    Boutros, M., Mlodzik, M. (2009). Electrochemical cues regulate assembly of
    the Frizzled/Dishevelled complex at the plasma membrane during planar
    epithelial polarization. Nature Cell Biology 11:286-94.
  • Farfsing, A., Engel, F., Seiffert, M., Hartmann, E., Ott, G., Rosenwald, A.,
    Stilgenbauer, S., Döhner, H., Boutros, M., Lichter, P., Pscherer, A. (2009).
    Gene knockdown studies revealed CCDC50 as a candidate gene in mantle
    cell lymphoma and chronic lymphocytic leukemia. Leukemia 11:2018-26.
  • Davidson, G., Shen, J., Huang, Y.L., Su, Y., Karaulanov, E., Bartscherer, K.,
    Hassler, C., Stannek, P., Boutros, M., Niehrs, C. (2009). Cell cycle control
    of Wnt receptor activation. Developmental Cell 17:788-99.
  • Kuttenkeuler, D., Pelte, N., Ragab, A., Gesellchen, V., Schneider, L., Blass,
    C., Axelsson, E., Huber, W., Boutros, M. (2010). A Large-Scale RNAi Screen
    Identifies Deaf1 as a Regulator of Innate Immune Responses in Drosophila.
    Journal of Innate Immunity 2:181-194.
  • Gilsdorf, M., Horn, T., Arziman, Z., Pelz, O., Kiner E., Boutros, M. (2010).
    GenomeRNAi: a database for cell-based RNAi phenotypes. 2009 Update.
    Nucleic Acids Research 38:D448-52.
  • Mleczko-Sanecka, K., Casanovas, G., Ragab, A., Breitkopf, K., Muller, A.,
    Boutros, M., Dooley, S., Hentze, M.W., Muckenthaler, M.U. (2010). SMAD7
    controls iron metabolism as a potent inhibitor of hepcidin expression. Blood
    115:2657-65.
  • Cruciat, C.M., Ohkawara, B., Acebron, S.P., Karaulanov, E., Reinhard, C.,
    Ingelfinger, D., Boutros, M., Niehrs, C. (2010). Requirement of prorenin
    receptor an vacuolar H+-ATPase-mediated acidification for Wnt signaling.
    Science 327:459-63.
  • Pau, G., Fuchs, F., Sklyar, O., Boutros, M., Huber, W. (2010). EBImage - an
    R package for image processing with applications to cellular phenotypes.
    Bioinformatics 26:979-81.
  • Falschlehner, C., Steinbrink, S., Erdmann, G., Boutros, M. (2010).
    High-throughput RNAi screening to dissect cellular pathways: a how-to
    guide. Biotechnology Journal 5:368-76.
  • Gobert, V., Osman, D., Bras, S., Auge, B., Boube, M., Bourbon, H.M., Horn,
    T., Boutros, M., Haenlin, M., Waltzer, L. (2010). A genome-wide RNA
    interference screen identifies a differential role of the Mediator CDK8
    module subunits for GATA/RUNX-activated transcription in Drosophila.
    Molecular Cellular Biology 30:2837-48.
  • Pelz., O., Gilsdorf, M., Boutros, M. (2010). web cellHTS: A web application
    for the analysis of high-throughput screening data. BMC Bioinformatics
    11:185.
  • Horn, T., Boutros, M. (2010). E-RNAi: a web application for the multi-
    species design of RNAi reagents—2010 update. Nucleic Acids Research
    38:W332-9.
  • Saj, A., Arziman, Z., Stempfle, D., van Belle, W., Sauder, U., Horn, T.,
    Dürrenberger, M., Paro, R., Boutros, M., Merdes, G. (2010). A combined ex
    vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an
    extensive notch interaction network. Developmental Cell 18:862-76.
  • Leber, B., Maier, B., Fuchs, F., Chi, J., Riffel, P., Anderhub, S., Wagner, L.,
    Ho, A.D., Salisbury, J.L., Boutros, M., Krämer, A. (2010). Proteins required
    for centrosome clustering in cancer cells. Science Translational Medicine
    2:33ra38.
  • Horn, T., Sandmann, T., Boutros, M. (2010).  Design and evaluation of
    genome-wide libraries for RNAi screens. Genome Biology 11:R61.
  • Fuchs, F., Pau, G., Kranz, D., Sklyar, O., Budjan, C., Steinbrink, S., Horn,
    T., Pedal, A., Huber, W., Boutros, M. (2010). Clustering phenotype
    populations by genome-wide RNAi and multiparametric imaging.
    Molecular Systems Biology 6:370.
  • Buechling, T., Bartscherer, K., Ohkawara, B., Chaudhary, V., Spirohn, K.,
    Niehrs, C., Boutros, M. (2010).  Wnt/Frizzled signaling requires dPRR, the
    Drosophila homolog of the prorenin receptor.
    Current Biology 20:1263-1268.
  • Niehrs, C., Boutros, M. (2010). Trafficking, Acidification and Growth Factor
    Signaling. Science Signaling 3:pe26.
  • Müller, H., Schmidt, D., Steinbrink, S., Mirgorodskaya, E., Lehmann, V., 
    Habermann, K., Dreher, F., Gustavsson, N., Kessler, T., Lehrach, H.,
    Herwig, R., Gobom, J., Ploubidou, A., Boutros, M., Lange, B.M. (2010).
    Proteomic and functional analysis of the mitotic Drosophila centrosome.
    EMBO Journal 29:3344-57.
  • Gilboa, L., Boutros, M. (2010). Celebrating 100 years of Drosophila
    research. EMBO reports 11:724-726.
  • Metzig, M., Nickles, D., Boutros, M. (2011). Large-scale RNAi screens to
    dissect TNF and NF-κB signaling pathways. Adv Exp Med Biol. 691:131-9.
  • Metzig, M., Nickles, D., Falschlehner, C., Lehmann-Koch, J., Straub, B.K.,
    Roth, W., Boutros, M. (2011). An RNAi screen identifies USP2 as a factor
    required for TNF-α induced NF-κB signaling. Int Journal of Cancer
    29:607-18.
  • Liang, J., Fu, Y., Cruciat, C.M., Jia, S., Wang, Y., Tong, Z., Tao, Q.,
    Ingelfinger, D., Boutros, M., Meng, A., Niehrs, C., Wu, W. (2011).
    Transmembrane Protein 198 Promotes LRP6 Phosphorylation and Wnt
    Signaling Activation. Molecular Cellular Biology 31:2577-90.
  • Huhn, S., Ingelfinger, D., Bermejo, J.L., Bevier, M., Pardini, B., Naccarati,
    A., Steinke, V., Rahner, N., Holinski-Feder, E., Morak, M., Schackert, H.K.,
    Görgens, H., Pox, C.P., Goecke, T., Kloor, M., Loeffler, M., Büttner, R.,
    Vodickova, L., Novotny, J., Demir, K., Cruciat, C.M., Renneberg, R., Huber,
    W., Niehrs, C., Boutros, M., Propping, P., Vodièka, P., Hemminki, K., Försti,
    A. (2011). Polymorphisms in CTNNBL1 in relation to colorectal cancer with
    evolutionary implications. Int J Mol Epidemiol Genet. 2:36-50.
  • Kondylis, V., Tang, Y., Fuchs, F., Boutros, M., Rabouille, C. (2011).
    Identification of ER Proteins involved in the Functional Organisation of the
    Early Secretory Pathway in Drosophila Cells by a Targeted RNAi Screen.
    PLoS One 6:e17173.
  • Ragab, A., Buechling, T., Gesellchen, V., Spirohn, K., Boettcher, A.-L.,
    Boutros, M. (2011). Drosophila Ras/MAPK signalling regulates innate
    immune responses in immune and intestinal stem cells. EMBO Journal
    30:1123-36
  • Horn, T., Sandmann, T., Fischer, B., Axelsson, E., Huber, W., Boutros, M.
    (2011). Mapping of signalling networks through synthetic genetic
    interaction analysis by RNAi. Nature Methods 8:341-6.
  • Axelsson, E., Sandmann, T., Horn, T., Boutros, M., Huber, W., Fischer, B.
    (2011).  Extracting quantitative genetic interaction phenotypes from matrix
    combinatorial RNAi. BMC Bioinformatics 12:342.
  • Sandmann, T., Vogg, M.C., Owlarn, S., Boutros, M., Bartscherer, K. (2011).
    The head-regeneration transcriptome of the planarian Schmidtea
    mediterranea. Genome Biology 12:R76
  • Zacharogianni, M., Kondylis, V., Tang, Y., Farhan, H., Xanthakis, D., Fuchs,
    F., Boutros, M., Rabouille, C. (2011). ERK7 is a negative regulator of
    protein secretion in response to amino acid starvation. EMBO Journal
    30:3684-700.
  • Glinka, A., Dolde, C., Kirsch, N., Huang, Y.L., Kazanskaya, O., Ingelfinger,
    D., Boutros, M., Cruciat, C.-M., Niehrs, C. (2011). LGR4 and LGR5 are
    R-spondin receptors mediating Wnt/beta-catenin and Wnt/PCP signaling.
    EMBO reports 12:1055-61.
  • Gilbert, D.F., Erdmann, G., Zhang, X., Fritzsche, A., Demir, K., Jaedicke, A.,
    Muehlenberg, K., Wanker, E.E., Boutros, M. (2011). A Novel Multiplex Cell
    Viability Assay for High-Throughput RNAi Screening. PLoS One 6:e28338.
  • Buechling, T., Boutros, M. (2011). Wnt signaling signaling at and above the
    receptor level. Curr Top Dev Biol. 97:21-53.
  • Buechling, T., Chaudhary, V., Spirohn, K., Weiss, M., Boutros, M. (2011).
    p24 proteins are required for secretion of Wnt ligands. EMBO reports,
    12:1265-72.
  • Augustin, I., Goidts, V., Bongers, A., Kerr, G., Vollert, G., Radlwimmer, B.,
    Hartmann, C., Herold-Mende, C., Reifenberger, G., von Deimling, A.,
    Boutros, M. (2012). The Wnt secretion protein Evi/Gpr177 promotes glioma
    tumorigenesis. EMBO Molecular Medicine 4:38-51.
  • Shamu, C., Wiemann, S., Boutros, M. (2012). On target: Call for a public
    repository for large-scale RNAi experiments. Nature Cell Biology, 14:115.
  • Sandmann, T., Boutros, M. (2012). Screens, Maps and Networks: from
    genome sequences to personalized medicine. Current Opinions in Genetics
    and Development 22:36-44.
  • Falschlehner, C., Boutros, M. (2012). Innate immunity: regulation of
    caspases by IAP-dependent ubiquitylation. EMBO Journal 31:2750-2.
  • Icardi, L., Moria, R., Gesellchen, V., Eyckerman, S., De Cauwer, L.,
    Verhelst, J., Vercauterene, K., Saelens, X., Meulemane, P., Leroux-Roelse,
    G., De Bosscher, K., Boutros, M., Tavernier, J. (2012). Sin3a repressor
    complex is a master regulator of STAT transcriptional activity.
    Proc. Natl. Acad. Sci. 109:12058-63.
  • Nickles, D., Falschlehner, C., Metzig, M., Boutros, M. (2012). A
    genome-wide RNAi screen identifies CASP4 as a factor required for tumor
    necrosis factor alpha signaling. Molecular and Cellular Biology 32:3372-81.
  • Demir, K., Boutros, M. (2012). Cell perturbation screens for target
    identification by RNAi. Methods Molecular Biology 910:1-13.
  • Stender, J.D., Pascual, G., Liu, W., Kaikkonen, M.U., Do, K., Spann, N.J.,
    Boutros, M., Perrimon, N., Rosenfeld, M.G., Glass, C.K. (2012). Control of
    proinflammatory gene programs by regulated trimethylation and
    demethylation of histone H4K20. Molecular Cell 48:28–38.
  • Petrakis, S., Raskó, T., Russ, J., Stroedicke, M., Riechers, S., Friedrich, R.
    P., Möller, A., Reinhardt, A., Vinayagam, A., Schaefer, M., Boutros, M.,
    Tricoire, H., Andrade-Navarro, M. A., Wanker, E. E. (2012). Identification of
    Human Proteins that Modify Misfolding and Proteotoxicity of Pathogenic
    Ataxin-1. PLoS Genetics 8:e1002897.
  • Gross, J., Chaudhary, V., Bartscherer, K., Boutros, M. (2012). Active Wnt
    proteins are secreted on exosomes. Nature Cell Biology 14:1036-45.
  • Horn, T., Boutros, M. (2012). Design of RNAi Reagents for Invertebrate
    Model Organisms and Human Disease Vectors. Methods Molecular Biology
    942:315-46.
  • Brechmann, M., Mock, T., Nickles, D., Kiessling, M., Weit, N., Breuer, R.,
    Müller, W., Wabnitz, G., Frey, F., Nicolay, J.P., Booken, N., Samstag, Y.,
    Klemke, C.D., Herling, M., Boutros, M., Krammer, P.H., Arnold, R. (2012).
    A PP4 Holoenzyme Balances Physiological and Oncogenic Nuclear
    Factor-Kappa B Signaling in T Lymphocytes. Immunity 37:697-708.
  • Page, R.M., Münch, A., Horn, T., Kuhn, P.H., Colombo, A., Reiner, O.,
    Boutros, M., Steiner, H., Lichtenthaler, S.F., Haass, C. (2012). Loss of
    PAFAH1B2 Reduces Amyloid-β Generation by Promoting the Degradation of
    Amyloid Precursor Protein C-Terminal Fragments. Journal of Neuroscience
    32:18204-18214.
  • Erdmann, G., Volz, C., Boutros, M. (2012). Systematic approaches to
    dissect biological processes in stem cells by image-based screening.
    Biotechnology Journal 7:768-78.
  • Schmidt, E.E., Pelz, O., Buhlmann, S., Kerr, G., Horn, T., Boutros, M.
    (2013). GenomeRNAi: a database for cell-based and in vivo RNAi
    phenotypes, 2013 update. Nucleic Acids Research 41:D1021–D1026.
  • Kuhns, S., Schmidt, K.N., Reymann, J., Gilbert, D.F., Neuner, A., Hub, B.,
    Carvalho, R., Wiedemann, P., Zentgraf, H., Erfle, H., Klingmüller, U.,
    Boutros, M., Pereira, G. (2013). The microtubule affinity regulating kinase
    MARK4 promotes axoneme extension during early ciliogenesis. Journal of
    Cell Biology 200:505–522.
  • Laufer, C., Fischer, B., Billmann, M., Huber, W., Boutros, M. (2013).
    Mapping genetic interactions in human cancer cells with RNAi and
    multiparametric phenotyping. Nature Methods 10:427–431.
  • Gross, J.C., Boutros, M., (2013). Secretion and extracellular space travel of
    Wnt proteins. Current Opinions in Genetics and Development 23:385-390.
  • Fukuyama, H., Verdier, Y., Guan, Y., Makino-Okamura, C., Shilova, V., Liu,
    X., Maksoud, E., Matsubayashi, J., Haddad, I., Spirohn, K., Ono, K., Hetru,
    C., Rossier, J., Ideker, T., Boutros, M., Vinh, J., Hoffmann, J.A. (2013).
    Landscape of protein-protein interactions in Drosophila immune deficiency
    signaling during bacterial challenge. Proc. Natl. Acad. Sci.  110:10717-22.
  • Serysheva, E., Berhane, H., Grumolato, L., Demir, K., Balmer, S., Bodak,
    M., Boutros, M., Aaronson, S., Mlodzik, M., Jenny, A. (2013). Wnk kinases
    are positive regulators of canonical Wnt/-catenin signaling. EMBO reports
    14:718-25.
  • Augustin, I., Gross, J., Baumann, D., Korn, C., Kerr, G., Grigoryan, T.,
    Mauch, C., Birchmeier, W., Boutros, M. (2013). Loss of epidermal Evi/Wls
    results in a phenotype resembling psoriasiform dermatitis. Journal of
    Experimental Medicine 210:1761-77.
  • Börner, K., Niopek, D., Cotugno, G., Kaldenbach, M., Pankert, T.,
    Willemsen, J., Zhang, X., Mockenhaupt, S., Schürmann, N., Serva, A, Hiet,
    M.-S., Wiedtke, E., Castoldi, M., Bauer, A., Starkuviene, V., Erfle, H.,
    Schnitzler, P., Muckenthaler, M., Gilbert, D., Bartenschlager, R., Boutros,
    M., Binder, M., Streetz, K., Kräusslich, H.-G., Grimm, D. (2013). Robust
    RNAi enhancement via human Argonaute-2 overexpression from plasmids,
    viral vectors and cell lines. Nucleic Acids Research 41:e199.
  • Heigwer, F., Kerr, G., Walther, N., Glaeser, K., Pelz, O., Breinig, M.,
    Boutros, M. (2013). E-TALEN: a web tool to design TALENs for genome
    engineering. Nucleic Acids Research 41:e190
  • Demir, K., Kirsch, N., Beretta, C.A., Erdmann, G., Ingelfinger, D., Moro, E.,
    Argenton, F., Carl, M., Niehrs, C., Boutros, M. (2013). RAB8B is required for
    activity and caveolar endocytosis of LRP6. Cell Reports 4:1224-34.
  • Voloshanenko, O., Erdmann, G., Dubash, T.D., Augustin, I., Metzig, M.,
    Moffa, G., Hundsrucker, C., Kerr, G., Sandmann, T., Anchang, B., Demir,
    K., Boehm, C., Leible, S., Ball, C.R., Glimm, H., Spang, R., Boutros, M.
    (2013). Wnt secretion is required to maintain high levels of Wnt activity in
    colon cancer cells. Nature Communications 4:2610.
  • Zhang, X., Boutros, M. (2013). A novel phenotypic dissimilarity method for
    image-based high-throughput screens. BMC Bioinformatics 14:336.
  • Mleczko-Sanecka, K., Roche, F., da Silva, A. R., Call, D., D'Alessio, F.,
    Ragab, A., Lapinski, F.E., Ummani, R., Korf, U., Oakes, C.C., Damm, G.,
    D’Alessandro, L., Klingmüller, U., King, P.D., Boutros, M., Hentze, M.W.,
    Muckenthaler, M. U. (2014). Unbiased RNAi screen for hepcidin regulators
    links hepcidin suppression to the proliferative Ras/RAF and the nutrient-
    dependent mTOR signaling pathways. Blood 123:1574-85.
  • Kranz, D., Boutros, M. (2014). A synthetic lethal screen identifies FAT1 as
    an antagonist of caspase-8 in extrinsic apoptosis. EMBO Journal 33:181-97.
  • Heigwer, F., Kerr, G., Boutros, M. (2014). E-CRISP: fast CRISPR target site
    identification. Nature Methods 11:122-3.
  • Jankovics, F., Henn, L., Bujna, A., Vilmos, P., Spirohn, K., Boutros, M.,
    Erdélyi, M. (2014). Functional analysis of the Drosophila embryonic germ
    cell transcriptome by RNA interference. PLoS ONE 9:e98579.
  • Kumar, S., Igman, M., Patel, T.R., Trageser, B., Gross, J.C., Rahm, K.,
    Boutros, M., Gradl, D., Steinbeisser, H., Holstein, T., Stetefeld, J., Ozbek,
    S. Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and
    modulatory determinants of wnt signaling activity. BMC Biology 12:44.
  • Korn, C., Scholz, B., Hu, J., Srivastava, K., Wojtarowicz, J., Arnsperger, T.,
    Adams, R.H., Boutros, M., Augustin, H.G., Augustin, I. (2014). Endothelial
    cell-derived non-canonical Wnt ligands control vascular pruning in
    angiogenesis. Development 141:1757-1766.
  • Laufer, C., Fischer, B., Huber, W., Boutros, M. (2014). Measuring genetic
    interactions in human cells by RNAi and imaging. Nature Protocols
    9:2341–2353.
  • Zhou, J., Florescu, S., Boettcher, A.-L., Luo, L., Dutta, D., Kerr, G., Cai, Y.,
    Edgar, B. A., Boutros, M. (2015). Dpp/Gbb signaling is required for normal
    intestinal regeneration during infection. Developmental Biology
    399:189-203.
  • Khandelwal, N., Breinig, M., Speck, T., Michels, T., Kreutzer, C., Sorrentino,
    A., Sharma, A. K., Umansky, L., Conrad, H., Poschke, I., Offringa, R.,
    König, R., Bernhard, H., Machlenkin, A., Boutros, M., Beckhove, P. (2015).
    A high-throughput RNAi screen for detection of immune-checkpoint
    molecules that mediate tumor resistance to cytotoxic T lymphocytes. EMBO
    Molecular Medicine 7:450-63.
  • Guida, C., Altamura, S., Klein, F. A., Galy, B., Boutros, M., Ulmer, A. J.,
    Hentze, M. W., Muckenthaler, M. U. (2015). A novel inflammatory pathway
    mediating rapid hepcidin-independent hypoferremia. Blood 125:2265-75.
  • Fischer, B., Sandmann, T., Horn, T., Billmann, M., Chaudhary, V., Huber,
    W., Boutros, M. (2015). A Map of Directional Genetic Interactions in a
    Metazoan Cell. eLife 4 doi: 10.7554/eLife.05464.
  • Tiebe, M., Lutz, M., De La Garza1, A., Buechling, T., Boutros, M., Teleman,
    A.A. (2015). REPTOR and REPTOR-BP regulate organismal metabolism and
    transcription downstream of mTORC1. Developmental Cell 33:272-84.
  • Betge, J., Kerr, G., Miersch, T., Leible, S., Erdmann, G., Galata, C.L., Zhan,
    T., Gaiser, T., Post, S., Ebert, M.P., Horisberger, K., Boutros, M. (2015).
    Amplicon sequencing of colorectal cancer: variant calling in frozen and
    formalin-fixed samples. PLoS ONE 10:e0127146.
  • Erdmann, G., Suchanek, M., Horn, P., Graf, F., Volz, C., Horn, T., Zhang,
    X., Wagner, W., Ho, A.D., Boutros, M. (2015). Functional fingerprinting of
    human mesenchymal stem cells using high-throughput RNAi screening.
    Genome Medicine 7:46.

Book Chapters and Books

more

Systems Genetics

Patents

  • Boutros, M. and Augustin, I. GPR177 as a target and marker in tumors. US9062114B2 
  • Fuchs, F., Gilbert, D., Koch, C., Maskey, R., Steinbrink, S. and Boutros, M. Tricyclic pyrimidine derivatives as Wnt antagonists. EP2445913B1

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