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Suchergebnis: RIP-Seq_b060__1_.pdf
SILAC B060 Jan Meier Michael Rogers j.meier@dkfz.de SILAC Taken from: Lößner et al.; “Quantitative Proteomics Identify Novel miR-155 Target Proteins”; PLOS One; 2011 For SILAC analysis, SILAC…
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Suchergebnis: PAT_protocol_a290.pdf
PAT Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in well, dice with…
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Suchergebnis: poly_A__tail_assay_a290.pdf
Poly(A) tail assay Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in…
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Suchergebnis: RNA_IP_Tissue_protocol_a290.pdf
RNA IP Tissue Protocol IP RNA Preparation of the beads 50µL Magnetic Prot A beads per condition (Dynabeads® Protein A, Invitrogen) Wash 2 x with PBS‐DEPC Block with yeast tRNA 50µg…
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Suchergebnis: Fracationation_Tissue_protocol_a290.pdf
Tissue Polysome Fractionation Tissue Polysome Fractionation (Stefan’s Protocol) Reagents HBSS+: HBSS + 1:50 CHX o 20ml/mouse Homogenization Buffer (HB): 1:50 CHX + 1:100 DTT + 1:50…
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Suchergebnis: Polysome_fractionation_a200.pdf
Polysome fractionation with RNA purification Polysome fractionation with RNA purification Johanna Schott, Georg Stoecklin lab, 2013 Material: Sucrose Beckman Centrifuge Tubes, 11 x 60 mm (328874)…
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Suchergebnis: RNA-IP_a200.pdf
RNA-Immunoprecipitation (RNA-IP) Kathrin Leppek, Georg Stoecklin lab, 2013 Small scale RNA-IP to confirm mRNA targets of RNA-binding proteins by RT-qPCR. In this case RAW264.7 macrophages were used…
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Suchergebnis: mRNA_half-life_measurement_a200.pdf
mRNA half‐life measurement in mammalian cells Messenger RNA half‐life measurements in mammalian Cells Sahil Sharma, Georg Stoecklin Lab, 2013 mRNA half‐life can be determined using Northern blot…
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Suchergebnis: Flouresence_in_situ_hybridization_a200.pdf
Flourescence in situ hybridization for detection of poly(A) RNA Fluorescence in situ hybridization for detection of poly(A) RNA Material: 1x PBS 4% Paraformaldehyde (PFA) in 1x PBS…
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Suchergebnis: In_vitro_RNA-protein_binding_AG_Hofmann_2012_a190.pdf
In vitro RNA-protein binding assay A190 Ilse Hofmann i.hofmann@dkfz-heidelberg.de AG Hofmann Nov 2012 In vitro RNA-protein binding assay The following samples are required for the assay: • • …
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