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Suchergebnis: CCU Molecular Hematology/Oncology - Translational Research
Die weiterführenden Inhalte sind nur auf englisch verfügbar.
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Suchergebnis: CCU Molecular Hematology/Oncology - Clinical Research
Die weiterführenden Inhalte sind nur auf englisch verfügbar.
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Suchergebnis: RNA-TRIZOL_d090.pdf
RNA Trizol RNA isolation from cells or tissues with TRIZOL A. Required reagents: DEPC‐treated water (Ambion) TRIzol Reagent (Invitrogen) Ice cold PBS 70% ethanol 100% ethanol (kept at ‐20) Always…
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Suchergebnis: Reverse_transcription_and_real-time_PCR_miRNA_C080.pdf
Rverse transcription and real time PCR miRNA Reverse transcription (RT) Reverse transcription (RT) reactions are performed using TaqMan miRNA Reverse Transcription Kit (Applied Biosystems, Germany)…
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Suchergebnis: TLDA_arrays_plasma_samples_c080.pdf
TILDA arrays plasma samples TLDA: TaqMan® Array MicroRNA Cards 1. Megaplex™ RT Reactions In this step, use the TaqMan® MicroRNA Reverse Transcription Kit and the Megaplex™ RT Primers to synthesize…
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Suchergebnis: Immunoprecipitation_with_AGO_c080.pdf
Immunoprecipitation with AGO Protocol: Immunoprecipitation Kit: Pierce Classic IP Kit Additional materials: 1. For cell culture 2. 0.1M Na2HPO4, 0.15M NaCl2, Ph 7.2 3. Antibody: anti‐AGO1 (clone…
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Suchergebnis: miRNA_extraction_protocols_c080.pdf
miRNA extraction 1. miRNA extraction from liquid samples (plasma/serum) 1. 2. 3. 4. defrost the plasma/serum sample on ice (do not vortex, but invert to mix) transfer 400 ul of plasma into fresh…
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Suchergebnis: miRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdfmiRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdf PDF, 80.37 KB Dokument Inhaltstyp: Dokument
MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Protocol: MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Seed 2.5×105 cells per well of a 6‐well plate 24 h before transfection.…
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Suchergebnis: RNA_affinity_purification_b150.pdf
RNA affinity purification Cytoplasmic Extract Preparation Forty 15 cm plates of 90 % confluent cells were washed twice with 1x PBS, scraped off the plates in 10 ml of 1x PBS and collected into 50 ml…
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Suchergebnis: PhenolExtraction_b066.pdf
Phenol Extraction Phenol / Chloroform / Isoamyl alcohol Volume of your sample (vol) Add 1/10 vol of 5M NaCl – you can increase the volume (vol) here by adding 0.5 M NaCl so that you loose less…
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