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Suchergebnis: RiboZero_b066.pdf
RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Ribo-Zero™ Magnetic Kit* (Human/Mouse/Rat) Cat. No. MRZH116 – 6 Reactions (Contains 1 box of Cat. No. RZH1046 and 1 box of Cat. No.…
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Suchergebnis: RNA_ISOLATION_b066.pdf
RNA Isolation RNA ISOLATION Main mix: (cover with aluminium foil): 4M guanidinisothiocyanat 25 mM sodium citrate pH 7 0,5% Sarcosyl Lauroylsacosine 20 ml 500 µl 100 µl (300 µl of 30% Sodium salt…
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Suchergebnis: Sucrose_gradient_b066.pdf
Sucrose gradient with long chromatin fragments (RNAse free) Sucrose gradient with long chromatin fragments (RNase free) Day 1 Prepare the gradient: Prepare a 5% and a 30% sucrose solution…
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Suchergebnis: userguide_encore_complete_rnaseq_b066.pdf
B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de USER GUIDE Encore Complete RNA-Seq Library Systems ® PART NOS. 0311, 0312, 0313, 0333 AND 0334 Patents, Licensing and Trademarks ©…
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Suchergebnis: NEB_Small_RNAs_b066.pdf
Small RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de SAMPLE PREPARATION NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (1–12) Instruction Manual NEB #E7300S/L 24/96…
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Suchergebnis: DNAGelExtraction_b066.pdf
DNA Gel Extraction DNA Gel extraction Under the UV light, cut out the piece of gel you are interested in. Put in a tube, that is big enough to contain the gel + buffer volume. Weight the piece of…
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Suchergebnis: FISH_TERRA_von_Katha_b066.pdf
Terra Fish with Dig‐labeled DNA probe + IF TERRA FISH with Dig‐labeled DNA probe + IF (Katharina Deeg, AG Rippe) DAY 1 - cells are grown on cover slips and incubated with CSK buffer for 5 min on…
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Suchergebnis: Click-iT-RNA_b066.pdf
Click-iT® RNA Imaging Kits B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Click-iT® RNA Imaging Kits Catalog nos. C10329, C10330 Table 1. Contents and storage information. Material …
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Suchergebnis: Methods_B063_DW_20121022_b063.pdf
Isolations, screening, sequencing Isolation of DNA and RNA (including miRNA) from tissue DNA and RNA including miRNA are isolated from tissues using the DNA/RNA All prep kit (Qiagen, Hilden,…
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Suchergebnis: 121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf PDF, 211.62 KB Dokument Inhaltstyp: Dokument
Daniel Mertens In vivo processing assay based on dual luciferase reporter system to evaluate DROSHA enzymatic activity Summary Luciferase reporter assays are widely used to study promoter activity,…
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