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Searchresult: DNAGelExtraction_b066.pdf
DNA Gel Extraction DNA Gel extraction Under the UV light, cut out the piece of gel you are interested in. Put in a tube, that is big enough to contain the gel + buffer volume. Weight the piece of…
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Searchresult: FISH_TERRA_von_Katha_b066.pdf
Terra Fish with Dig‐labeled DNA probe + IF TERRA FISH with Dig‐labeled DNA probe + IF (Katharina Deeg, AG Rippe) DAY 1 - cells are grown on cover slips and incubated with CSK buffer for 5 min on…
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Searchresult: Click-iT-RNA_b066.pdf
Click-iT® RNA Imaging Kits B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Click-iT® RNA Imaging Kits Catalog nos. C10329, C10330 Table 1. Contents and storage information. Material …
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Searchresult: Methods_B063_DW_20121022_b063.pdf
Isolations, screening, sequencing Isolation of DNA and RNA (including miRNA) from tissue DNA and RNA including miRNA are isolated from tissues using the DNA/RNA All prep kit (Qiagen, Hilden,…
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Searchresult: 121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf PDF, 211.62 KB Document content type: Document
Daniel Mertens In vivo processing assay based on dual luciferase reporter system to evaluate DROSHA enzymatic activity Summary Luciferase reporter assays are widely used to study promoter activity,…
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Searchresult: RIP-Seq_b060__1_.pdf
SILAC B060 Jan Meier Michael Rogers j.meier@dkfz.de SILAC Taken from: Lößner et al.; “Quantitative Proteomics Identify Novel miR-155 Target Proteins”; PLOS One; 2011 For SILAC analysis, SILAC…
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Searchresult: PAT_protocol_a290.pdf
PAT Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in well, dice with…
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Searchresult: poly_A__tail_assay_a290.pdf
Poly(A) tail assay Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in…
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Searchresult: RNA_IP_Tissue_protocol_a290.pdf
RNA IP Tissue Protocol IP RNA Preparation of the beads 50µL Magnetic Prot A beads per condition (Dynabeads® Protein A, Invitrogen) Wash 2 x with PBS‐DEPC Block with yeast tRNA 50µg…
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Searchresult: Fracationation_Tissue_protocol_a290.pdf
Tissue Polysome Fractionation Tissue Polysome Fractionation (Stefan’s Protocol) Reagents HBSS+: HBSS + 1:50 CHX o 20ml/mouse Homogenization Buffer (HB): 1:50 CHX + 1:100 DTT + 1:50…
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