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Searchresult: miRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdfmiRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdf PDF, 80.37 KB Document content type: Document
MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Protocol: MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Seed 2.5×105 cells per well of a 6‐well plate 24 h before transfection.…
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Searchresult: miRNA_extraction_protocols_c080.pdf
miRNA extraction 1. miRNA extraction from liquid samples (plasma/serum) 1. 2. 3. 4. defrost the plasma/serum sample on ice (do not vortex, but invert to mix) transfer 400 ul of plasma into fresh…
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Searchresult: Immunoprecipitation_with_AGO_c080.pdf
Immunoprecipitation with AGO Protocol: Immunoprecipitation Kit: Pierce Classic IP Kit Additional materials: 1. For cell culture 2. 0.1M Na2HPO4, 0.15M NaCl2, Ph 7.2 3. Antibody: anti‐AGO1 (clone…
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Searchresult: RNA_affinity_purification_b150.pdf
RNA affinity purification Cytoplasmic Extract Preparation Forty 15 cm plates of 90 % confluent cells were washed twice with 1x PBS, scraped off the plates in 10 ml of 1x PBS and collected into 50 ml…
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Searchresult: PhenolExtraction_b066.pdf
Phenol Extraction Phenol / Chloroform / Isoamyl alcohol Volume of your sample (vol) Add 1/10 vol of 5M NaCl – you can increase the volume (vol) here by adding 0.5 M NaCl so that you loose less…
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Searchresult: RiboZero_b066.pdf
RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Ribo-Zero™ Magnetic Kit* (Human/Mouse/Rat) Cat. No. MRZH116 – 6 Reactions (Contains 1 box of Cat. No. RZH1046 and 1 box of Cat. No.…
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Searchresult: RNA_ISOLATION_b066.pdf
RNA Isolation RNA ISOLATION Main mix: (cover with aluminium foil): 4M guanidinisothiocyanat 25 mM sodium citrate pH 7 0,5% Sarcosyl Lauroylsacosine 20 ml 500 µl 100 µl (300 µl of 30% Sodium salt…
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Searchresult: Sucrose_gradient_b066.pdf
Sucrose gradient with long chromatin fragments (RNAse free) Sucrose gradient with long chromatin fragments (RNase free) Day 1 Prepare the gradient: Prepare a 5% and a 30% sucrose solution…
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Searchresult: userguide_encore_complete_rnaseq_b066.pdf
B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de USER GUIDE Encore Complete RNA-Seq Library Systems ® PART NOS. 0311, 0312, 0313, 0333 AND 0334 Patents, Licensing and Trademarks ©…
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Searchresult: NEB_Small_RNAs_b066.pdf
Small RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de SAMPLE PREPARATION NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (1–12) Instruction Manual NEB #E7300S/L 24/96…
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