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Searchresult: Detection_of_cytosine_methylation_in_RNA_AGLyko_a130.pdfDetection_of_cytosine_methylation_in_RNA_AGLyko_a130.pdf PDF, 291.11 KB Document content type: Document
Detection of Cytosine Methylation in RNA Using Bisulfite Sequencing A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de Cite as: Cold Spring Harb…
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Searchresult: SOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdfSOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdf PDF, 120.00 KB Document content type: Document
Transfection of miRNA‐LNA inhibitors in MEF Reagents cell types: miRNA inhibitor: MEF miRCURY LNA™ microRNA Power Inhibitor 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot,…
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Searchresult: SOP_Transfection_of_miRNA_mimics_in_HUVECs_a100.pdf
Transfection of miRNA mimics in HUVECs Reagents cell types: miRNA inhibitor: HUVECs miRVana miRNA mimics 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot, store at ‐20°C…
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Searchresult: PAR_Clip_protocol_a030.pdf
PAR clip A030 Ingrid Grummt i.grummt@dkfz.de PROTOCOL OUTLINE 1. Protocol Title PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) - a step-by-step protocol…
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Searchresult: Protein_pull-down_with_immobilized_RNA_a030.pdf
Protein pull‐down with immobilized RNA Pull‐down of RNA binding proteins with immobilized biotinylated transcripts Buffers Cell lysis buffer: ◦ 85 mM M KCl ◦ 0.5% NP‐40 (Pierce) ◦ 5…
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Searchresult: RIP_protocol_a030.pdf
RNA immunoprecipitation (RIP) RNA immunoprecipitation (RIP) Use 5‐10x106 cells per RIP. Wash cells twice in cold PBS, harvest and pellet cells by centrifugation (3 min…
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Searchresult: RNA_pull-down_with_immobilized_proteins_a030.pdf
RNA pull‐down with GFP‐tagged immobilized proteins Pull‐down of RNA with GFP‐tagged immobilized proteins 1. Purification of GFP‐tagged proteins: Materials: Lysis buffer ◦ AM300 (AM100…
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Searchresult: Isolation-of-nascent-transcripts_a030.pdf
Isolation of nascent transcripts Protocoll for 4sU‐tagging, thiol‐specific biotinylation and separation of total RNA into nascent and pre‐existing RNA This protocol was completed on the 29.11.2010…
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Searchresult: ChRIP_protocola030.pdf
Chromatin Immunoprecipitation ChRIP Assay Chromatin Immunoprecipitation (ChRIP) Assay 1. Add 1% formaldehyde to the medium, 10 min at room temperature on a rocker 2. Add 125 mM glycine (5 min at RT)…
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Searchresult: rna_at_dkfz_meeting_2012.pdf
RNA@ Meeting: 18.07.2012, 14:00 – 19:00 Room K1 / K2 and Foyer of DKFZ Communication Center RNA@ PROGRAMME 14:00 – 15:30 Welcome Sven Diederichs 1 Maiwen Caudron‐Herger Maintenance of active…
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