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Searchresult: miRNA_extraction_protocols_c080.pdf
miRNA extraction 1. miRNA extraction from liquid samples (plasma/serum) 1. 2. 3. 4. defrost the plasma/serum sample on ice (do not vortex, but invert to mix) transfer 400 ul of plasma into fresh…
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Searchresult: miRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdfmiRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdf PDF, 80.37 KB Document content type: Document
MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Protocol: MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Seed 2.5×105 cells per well of a 6‐well plate 24 h before transfection.…
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Searchresult: Reverse_transcription_and_real-time_PCR_miRNA_C080.pdfReverse_transcription_and_real-time_PCR_miRNA_C080.pdf PDF, 78.66 KB Document content type: Document
Rverse transcription and real time PCR miRNA Reverse transcription (RT) Reverse transcription (RT) reactions are performed using TaqMan miRNA Reverse Transcription Kit (Applied Biosystems, Germany)…
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Searchresult: TLDA_arrays_plasma_samples_c080.pdf
TILDA arrays plasma samples TLDA: TaqMan® Array MicroRNA Cards 1. Megaplex™ RT Reactions In this step, use the TaqMan® MicroRNA Reverse Transcription Kit and the Megaplex™ RT Primers to synthesize…
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Searchresult: RNA_affinity_purification_b150.pdf
RNA affinity purification Cytoplasmic Extract Preparation Forty 15 cm plates of 90 % confluent cells were washed twice with 1x PBS, scraped off the plates in 10 ml of 1x PBS and collected into 50 ml…
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Searchresult: DNAGelExtraction_b066.pdf
DNA Gel Extraction DNA Gel extraction Under the UV light, cut out the piece of gel you are interested in. Put in a tube, that is big enough to contain the gel + buffer volume. Weight the piece of…
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Searchresult: FISH_TERRA_von_Katha_b066.pdf
Terra Fish with Dig‐labeled DNA probe + IF TERRA FISH with Dig‐labeled DNA probe + IF (Katharina Deeg, AG Rippe) DAY 1 - cells are grown on cover slips and incubated with CSK buffer for 5 min on…
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Searchresult: NEB_Small_RNAs_b066.pdf
Small RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de SAMPLE PREPARATION NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (1–12) Instruction Manual NEB #E7300S/L 24/96…
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Searchresult: PhenolExtraction_b066.pdf
Phenol Extraction Phenol / Chloroform / Isoamyl alcohol Volume of your sample (vol) Add 1/10 vol of 5M NaCl – you can increase the volume (vol) here by adding 0.5 M NaCl so that you loose less…
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Searchresult: RiboZero_b066.pdf
RNA B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Ribo-Zero™ Magnetic Kit* (Human/Mouse/Rat) Cat. No. MRZH116 – 6 Reactions (Contains 1 box of Cat. No. RZH1046 and 1 box of Cat. No.…
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