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Searchresult: RNA_ISOLATION_b066.pdf
RNA Isolation RNA ISOLATION Main mix: (cover with aluminium foil): 4M guanidinisothiocyanat 25 mM sodium citrate pH 7 0,5% Sarcosyl Lauroylsacosine 20 ml 500 µl 100 µl (300 µl of 30% Sodium salt…
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Searchresult: Sucrose_gradient_b066.pdf
Sucrose gradient with long chromatin fragments (RNAse free) Sucrose gradient with long chromatin fragments (RNase free) Day 1 Prepare the gradient: Prepare a 5% and a 30% sucrose solution…
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Searchresult: userguide_encore_complete_rnaseq_b066.pdf
B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de USER GUIDE Encore Complete RNA-Seq Library Systems ® PART NOS. 0311, 0312, 0313, 0333 AND 0334 Patents, Licensing and Trademarks ©…
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Searchresult: Click-iT-RNA_b066.pdf
Click-iT® RNA Imaging Kits B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de Click-iT® RNA Imaging Kits Catalog nos. C10329, C10330 Table 1. Contents and storage information. Material …
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Searchresult: Methods_B063_DW_20121022_b063.pdf
Isolations, screening, sequencing Isolation of DNA and RNA (including miRNA) from tissue DNA and RNA including miRNA are isolated from tissues using the DNA/RNA All prep kit (Qiagen, Hilden,…
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Searchresult: 121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf PDF, 211.62 KB Document content type: Document
Daniel Mertens In vivo processing assay based on dual luciferase reporter system to evaluate DROSHA enzymatic activity Summary Luciferase reporter assays are widely used to study promoter activity,…
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Searchresult: RIP-Seq_b060__1_.pdf
SILAC B060 Jan Meier Michael Rogers j.meier@dkfz.de SILAC Taken from: Lößner et al.; “Quantitative Proteomics Identify Novel miR-155 Target Proteins”; PLOS One; 2011 For SILAC analysis, SILAC…
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Searchresult: Fracationation_Tissue_protocol_a290.pdf
Tissue Polysome Fractionation Tissue Polysome Fractionation (Stefan’s Protocol) Reagents HBSS+: HBSS + 1:50 CHX o 20ml/mouse Homogenization Buffer (HB): 1:50 CHX + 1:100 DTT + 1:50…
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Searchresult: PAT_protocol_a290.pdf
PAT Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in well, dice with…
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Searchresult: poly_A__tail_assay_a290.pdf
Poly(A) tail assay Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in…
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