Proteomics
Dr. Dominic Helm
Head of Facility
The Proteomics Core Facility at the DKFZ offers services related to protein analysis for internal groups and their cooperation partners. From the digestion of the proteins to the measurement on our LC-MS instruments to the data analysis, we find out how the proteome of your samples is composed.
Services
We offer the following sample preparation services:
- Protocols for extraction buffers and preparation of ready-to-use buffers
- Provision of various devices for protein extraction (see Instrumentation)
- Bead-based protein digestion (SP3)
- SDS-PAGE, Coomassie staining and in-gel digestion
- Peptide purifications
Source SP3: Hughes, Christopher S., et al. "Single-pot, solid-phase-enhanced sample preparation for proteomics experiments." Nature protocols 14.1 (2019): 68-85.
Source In-gel digestion: Shevchenko, Andrej, et al. "In-gel digestion for mass spectrometric characterization of proteins and proteomes." Nature protocols 1.6 (2006): 2856-2860.
Our workflow
Instrumentation
Our LC-MS systems are equipped with Orbitrap-based mass spectrometers, the Orbitrap Exploris 480 from Thermo Fisher Scientific. These instruments are characterized by high sensitivity and scanning speed in combination with mass accuracy in the ppm range and high resolution and are therefore perfectly suited for high-throughput proteome analysis with optimal data quality.
We are eagerly awaiting the arrival of our new Orbitrap Astral mass spectrometers in 2025.
Team
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Dr. Dominic Helm
Head of Facility
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Frederic Bethke
Technician (LC-MS)
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Gerhard Erben
Engineer (LC-MS)
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Sabine Fiedler
Technician (Sample Preparation)
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Dr. Dario Frey
Postdoctoral Researcher
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Ramona Irsevic
Technician (Sample Preparation)
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Denise Jansen
PhD Student
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Anna Neuerburg
Technician (Sample Preparation)
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Naoki Sanzenbacher
Trainee
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Martin Schneider
Scientist (Bioinformatician)
Get in touch with us