The high throughput sequencing unit provides highly parallel DNA sequencing service based on the Illumina HiSeq 2000 / 2500 and 4000, MiSeq and HiSeq X as well as NovaSeq and NextSeq and PacBio Sequel.
The DKFZ Microarray Core Facility provide access to full service of state-of-the-art molecular profiling based on Illumina technology for members of DKFZ, NCT and their collaborators
MicroRNAs (miRNAs) are evolutionarily conserved, small, noncoding RNA molecules that negatively regulate gene expression at the level of translation in a variety of eukaryotic organisms. Although the first miRNA was described in 1993, but only recently the breadth and diversity of this gene class been uncovered. Vertebrate genomes encode as many as 1000 unique miRNAs, which are predicted to regulate expression of at least 30% of genes.
Epigenetics describes the study of heritable changes in gene function that occur without a change in the nuclear DNA sequence. In addition to RNA-associated silencing and histone modification, a major epigenetic mechanism in higher eukaryotes is DNA methylation.
Genotyping provides a measurement of the genetic variation between members of a species. Single nucleotide polymorphisms (SNP) are the most common type of genetic variation. A SNP is a single base pair mutation at a specific locus, usually consisting of two alleles.