Stable isogenic recombinant (Sir) cell lines

The stable isogenic cell line service provides highly standardized cell line models for functional analysis and high throughput screening. By integration of a specific recombination site in our host cell lines, inducible expression constructs can be stably inserted in a defined genomic locus. Compared to classical transfection based approaches the Sir cell line technology offers advantages which increase sensitivity and reproducibilitiy of the datasets.

Antibodies

The Unit offers the production of monoclonal (mouse/rat) antibodies using the hybridoma technology and the production of polyclonal (guinea pig) antibodies for research projects and as diagnostic tools in histopathology. In addition, we offer non-GMP production and purification of monoclonal antibodies.

Cell Contamination Control

Cell cultures are important tools for basic and applied biomedical research. Contaminations of cell culture pose a major threat to scientific results. In addition to molds, yeast and bacteria contaminations that are easily detectable by pH shifts, turbidity, and cell destruction, other contaminations such as viruses, mycoplasma and other cell lines are more difficult to detect, and as a result, are a potentially more serious problem. The need for contamination monitoring of any cell lines used for in vitro studies is increasingly emphasized (Lichter et al, 2010, Nature 515:7, Science 346:679) by granting agencies as well as by journals -> no cell-line authentification = no grant/publication.

Multiplex human Cell Authentication (MCA)

Cell cultures are important tools for basic and applied biomedical research. About 15-20% of human cell lines have been estimated to be mislabelled or affected by cross-contamination with other human cell lines. Moreover, the mandatory requirement for authentification of any cell lines used for in vitro studies is increasingly emphasized (Lichter et al, 2010, Nature 515:7, Science 346:679) by granting agencies as well as by journals -> no cell-line authentification = no grant/publication.

last update:
06/03/2019
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