Videos and References

A selection of short Youtoube videos about Microscale Thermophoresis
 

NanoTemper Technology: Learn about the handling of NT.115

 

short overview about the measurement steps (commercial)
duration: ~ 2 minutes

Measuring Interactions Using Microscale Thermophoresis by NanoTemper

 

Advantages, applications, physical principle, thermophoresis curves (commercial)
duration ~ 3 minutes

Protein Purification-free Binding Analysis by MicroScale Thermophoresis

 

purification-free measurement using GFP-tagged proteins
detailed workflow, detailed analysis/curve fitting examples
duration: ~ 10 miniutes

 

Tools

Concentration finder:  KD-Intervall Optimization

 

enter the assumed Kd range for your binding experiment to
find the optimal concentration range of the  ligand (titrant).

 

Consumables (capillaries & labelling kits)

Overview about Kits and Capillary Types

product description,
get an a quote for your consumables

 

 

Basic Literature

MST Starting Guide – Monolith NT.115   [>]
( i )  concise summary: assay setup and how to avoid common pitfalls

 

Microscale thermophoresis - Wikipedia  [>]

( i )  short introduction into the methodology

 

MicroScale Thermophoresis: Interaction analysis and beyond  [>]

( i )  more detailed introduction into MicroScale Thermophoresis
        showing a row of interesting examples

 

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last update:
28/02/2019
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