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Searchresult: AAV_CellStack_production_MBDfinal_a170.pdf
AAV Cellstack production A. Vegiopoulos; modified: M.Berriel Diaz 12/2011 Protocol for AAV production General Remarks: This protocol is designed for the production of two AAViruses in parallel. You…
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Searchresult: RIP_GFP_binder_AGLyko_a130.pdf
RIP GFB A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de RNA -Immunoprecipitation (RIP) using Dm cell extract and GFP-binder resin: The procedure…
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Searchresult: RIP_using_M2_FLAG_AGLyko_a130.pdf
RIP using F2 FLAG A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de RNA -Immunoprecipitation (RIP) using Dm cell extract and M2-FLAG resin: The…
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Searchresult: Detection_of_cytosine_methylation_in_RNA_AGLyko_a130.pdfDetection_of_cytosine_methylation_in_RNA_AGLyko_a130.pdf PDF, 291.11 KB Document content type: Document
Detection of Cytosine Methylation in RNA Using Bisulfite Sequencing A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de Cite as: Cold Spring Harb…
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Searchresult: Invitro_5mC_methylation_assay_Dnmt2_AGLyko_a130.pdf
In vitro methylation using Dnmt2 – Tritium-Assay A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de In vitro methylation using Dnmt2 – Tritium-Assay…
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Searchresult: SOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdfSOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdf PDF, 120.00 KB Document content type: Document
Transfection of miRNA‐LNA inhibitors in MEF Reagents cell types: miRNA inhibitor: MEF miRCURY LNA™ microRNA Power Inhibitor 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot,…
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Searchresult: SOP_Transfection_of_miRNA_mimics_in_HUVECs_a100.pdf
Transfection of miRNA mimics in HUVECs Reagents cell types: miRNA inhibitor: HUVECs miRVana miRNA mimics 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot, store at ‐20°C…
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Searchresult: RNA_pull-down_with_immobilized_proteins_a030.pdf
RNA pull‐down with GFP‐tagged immobilized proteins Pull‐down of RNA with GFP‐tagged immobilized proteins 1. Purification of GFP‐tagged proteins: Materials: Lysis buffer ◦ AM300 (AM100…
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Searchresult: Protein_pull-down_with_immobilized_RNA_a030.pdf
Protein pull‐down with immobilized RNA Pull‐down of RNA binding proteins with immobilized biotinylated transcripts Buffers Cell lysis buffer: ◦ 85 mM M KCl ◦ 0.5% NP‐40 (Pierce) ◦ 5…
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Searchresult: RIP_protocol_a030.pdf
RNA immunoprecipitation (RIP) RNA immunoprecipitation (RIP) Use 5‐10x106 cells per RIP. Wash cells twice in cold PBS, harvest and pellet cells by centrifugation (3 min…
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