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Searchresult: Molecular Hematology/Oncology
Our Research Our translational and clinical research focus on Cancer of Unknown Primary (CUP). CUP is a highly aggressive, primary metastatic malignancy, in which only metastases but no primary…
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Searchresult: RNA-TRIZOL_d090.pdf
RNA Trizol RNA isolation from cells or tissues with TRIZOL A. Required reagents: DEPC‐treated water (Ambion) TRIzol Reagent (Invitrogen) Ice cold PBS 70% ethanol 100% ethanol (kept at ‐20) Always…
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Searchresult: TLDA_arrays_plasma_samples_c080.pdf
TILDA arrays plasma samples TLDA: TaqMan® Array MicroRNA Cards 1. Megaplex™ RT Reactions In this step, use the TaqMan® MicroRNA Reverse Transcription Kit and the Megaplex™ RT Primers to synthesize…
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Searchresult: Reverse_transcription_and_real-time_PCR_miRNA_C080.pdfReverse_transcription_and_real-time_PCR_miRNA_C080.pdf PDF, 78.66 KB Document content type: Document
Rverse transcription and real time PCR miRNA Reverse transcription (RT) Reverse transcription (RT) reactions are performed using TaqMan miRNA Reverse Transcription Kit (Applied Biosystems, Germany)…
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Searchresult: Immunoprecipitation_with_AGO_c080.pdf
Immunoprecipitation with AGO Protocol: Immunoprecipitation Kit: Pierce Classic IP Kit Additional materials: 1. For cell culture 2. 0.1M Na2HPO4, 0.15M NaCl2, Ph 7.2 3. Antibody: anti‐AGO1 (clone…
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Searchresult: miRNA_extraction_protocols_c080.pdf
miRNA extraction 1. miRNA extraction from liquid samples (plasma/serum) 1. 2. 3. 4. defrost the plasma/serum sample on ice (do not vortex, but invert to mix) transfer 400 ul of plasma into fresh…
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Searchresult: miRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdfmiRNA_mimic_or_inhibitor_transfection_Lipofectamine_c080.pdf PDF, 80.37 KB Document content type: Document
MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Protocol: MicroRNA Mimic/Inhibitor Transfection with Lipofectamine Seed 2.5×105 cells per well of a 6‐well plate 24 h before transfection.…
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Searchresult: RNA_affinity_purification_b150.pdf
RNA affinity purification Cytoplasmic Extract Preparation Forty 15 cm plates of 90 % confluent cells were washed twice with 1x PBS, scraped off the plates in 10 ml of 1x PBS and collected into 50 ml…
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Searchresult: RNA_ISOLATION_b066.pdf
RNA Isolation RNA ISOLATION Main mix: (cover with aluminium foil): 4M guanidinisothiocyanat 25 mM sodium citrate pH 7 0,5% Sarcosyl Lauroylsacosine 20 ml 500 µl 100 µl (300 µl of 30% Sodium salt…
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Searchresult: Sucrose_gradient_b066.pdf
Sucrose gradient with long chromatin fragments (RNAse free) Sucrose gradient with long chromatin fragments (RNase free) Day 1 Prepare the gradient: Prepare a 5% and a 30% sucrose solution…
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