Suche

Sortierung:

Filter:
6348 Ergebnisse
  • Suchergebnis: RIP_using_M2_FLAG_AGLyko_a130.pdf
    RIP_using_M2_FLAG_AGLyko_a130.pdf PDF, 171.93 KB Inhaltstyp: Dokument

    RIP using F2 FLAG A130 Matthias Schäfer m.schaefer@dkfz-heidelberg.de Francesca Tuorto f.tuorto@dkfz-heidelberg.de RNA -Immunoprecipitation (RIP) using Dm cell extract and M2-FLAG resin: The…

    Dokument
  • Suchergebnis: SOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdf
    SOP_Transfection_of_LNA-miRNA-inhibitors_in_MEFs_a100.pdf PDF, 120.00 KB Inhaltstyp: Dokument

    Transfection of miRNA‐LNA inhibitors in MEF Reagents cell types: miRNA inhibitor: MEF miRCURY LNA™ microRNA Power Inhibitor 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot,…

    Dokument
  • Suchergebnis: SOP_Transfection_of_miRNA_mimics_in_HUVECs_a100.pdf
    SOP_Transfection_of_miRNA_mimics_in_HUVECs_a100.pdf PDF, 120.08 KB Inhaltstyp: Dokument

    Transfection of miRNA mimics in HUVECs Reagents cell types: miRNA inhibitor: HUVECs miRVana miRNA mimics 5 nmol, dilute 5 nmol in 250 µl H2O = 20 µM stock solution, aliquot, store at ‐20°C…

    Dokument
  • Suchergebnis: ChRIP_protocola030.pdf
    ChRIP_protocola030.pdf PDF, 90.35 KB Inhaltstyp: Dokument

    Chromatin Immunoprecipitation ChRIP Assay Chromatin Immunoprecipitation (ChRIP) Assay 1. Add 1% formaldehyde to the medium, 10 min at room temperature on a rocker 2. Add 125 mM glycine (5 min at RT)…

    Dokument
  • Suchergebnis: Isolation-of-nascent-transcripts_a030.pdf
    Isolation-of-nascent-transcripts_a030.pdf PDF, 186.12 KB Inhaltstyp: Dokument

    Isolation of nascent transcripts Protocoll for 4sU‐tagging, thiol‐specific biotinylation and separation of total RNA into nascent and pre‐existing RNA This protocol was completed on the 29.11.2010…

    Dokument
  • Suchergebnis: PAR_Clip_protocol_a030.pdf
    PAR_Clip_protocol_a030.pdf PDF, 771.66 KB Inhaltstyp: Dokument

    PAR clip A030 Ingrid Grummt i.grummt@dkfz.de PROTOCOL OUTLINE 1. Protocol Title PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) - a step-by-step protocol…

    Dokument
  • Suchergebnis: Protein_pull-down_with_immobilized_RNA_a030.pdf
    Protein_pull-down_with_immobilized_RNA_a030.pdf PDF, 205.36 KB Inhaltstyp: Dokument

    Protein pull‐down with immobilized RNA Pull‐down of RNA binding proteins with immobilized biotinylated transcripts Buffers  Cell lysis buffer:    ◦ 85 mM M KCl ◦ 0.5% NP‐40 (Pierce) ◦ 5…

    Dokument
  • Suchergebnis: RIP_protocol_a030.pdf
    RIP_protocol_a030.pdf PDF, 83.50 KB Inhaltstyp: Dokument

    RNA immunoprecipitation (RIP) RNA immunoprecipitation (RIP) Use 5‐10x106 cells per RIP.               Wash cells twice in cold PBS, harvest and pellet cells by centrifugation (3 min…

    Dokument
  • Suchergebnis: RNA_pull-down_with_immobilized_proteins_a030.pdf
    RNA_pull-down_with_immobilized_proteins_a030.pdf PDF, 180.10 KB Inhaltstyp: Dokument

    RNA pull‐down with GFP‐tagged immobilized proteins Pull‐down of RNA with GFP‐tagged immobilized proteins 1. Purification of GFP‐tagged proteins: Materials:   Lysis buffer ◦ AM300 (AM100…

    Dokument
  • Suchergebnis: rna_at_dkfz_meeting_2012.pdf
    rna_at_dkfz_meeting_2012.pdf PDF, 112.70 KB Inhaltstyp: Dokument

    RNA@ Meeting: 18.07.2012, 14:00 – 19:00 Room K1 / K2 and Foyer of DKFZ Communication Center RNA@ PROGRAMME 14:00 – 15:30 Welcome Sven Diederichs 1 Maiwen Caudron‐Herger Maintenance of active…

    Dokument
Mehr laden
Formular

Formulardaten werden geladen ...