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  • Suchergebnis: RNA_ISOLATION_b066.pdf
    RNA_ISOLATION_b066.pdf PDF, 90.78 KB Inhaltstyp: Dokument

    RNA Isolation RNA ISOLATION Main mix: (cover with aluminium foil): 4M guanidinisothiocyanat 25 mM sodium citrate pH 7 0,5% Sarcosyl Lauroylsacosine 20 ml 500 µl 100 µl (300 µl of 30% Sodium salt…

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  • Suchergebnis: Sucrose_gradient_b066.pdf
    Sucrose_gradient_b066.pdf PDF, 91.57 KB Inhaltstyp: Dokument

    Sucrose gradient with long chromatin fragments (RNAse free) Sucrose gradient with long chromatin fragments (RNase free) Day 1 Prepare the gradient:    Prepare a 5% and a 30% sucrose solution…

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  • Suchergebnis: userguide_encore_complete_rnaseq_b066.pdf
    userguide_encore_complete_rnaseq_b066.pdf PDF, 831.30 KB Inhaltstyp: Dokument

    B066 Maiwen Caudron-Herger m.caudron@dkfz-heidelberg.de USER GUIDE Encore Complete RNA-Seq Library Systems ® PART NOS. 0311, 0312, 0313, 0333 AND 0334  Patents, Licensing and Trademarks ©…

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  • Suchergebnis: Methods_B063_DW_20121022_b063.pdf
    Methods_B063_DW_20121022_b063.pdf PDF, 93.31 KB Inhaltstyp: Dokument

    Isolations, screening, sequencing Isolation of DNA and RNA (including miRNA) from tissue DNA and RNA including miRNA are isolated from tissues using the DNA/RNA All prep kit (Qiagen, Hilden,…

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  • Suchergebnis: 121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf
    121001_ivPA_assay_to_measure_Drosha_enzymatic_activity_variant_1_b061.pdf PDF, 211.62 KB Inhaltstyp: Dokument

    Daniel Mertens In vivo processing assay based on dual luciferase reporter system to evaluate DROSHA enzymatic activity Summary Luciferase reporter assays are widely used to study promoter activity,…

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  • Suchergebnis: RIP-Seq_b060__1_.pdf
    RIP-Seq_b060__1_.pdf PDF, 90.11 KB Inhaltstyp: Dokument

    SILAC B060 Jan Meier Michael Rogers j.meier@dkfz.de SILAC Taken from: Lößner et al.; “Quantitative Proteomics Identify Novel miR-155 Target Proteins”; PLOS One; 2011 For SILAC analysis, SILAC…

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  • Suchergebnis: Fracationation_Tissue_protocol_a290.pdf
    Fracationation_Tissue_protocol_a290.pdf PDF, 90.43 KB Inhaltstyp: Dokument

    Tissue Polysome Fractionation Tissue Polysome Fractionation (Stefan’s Protocol) Reagents       HBSS+: HBSS + 1:50 CHX o 20ml/mouse Homogenization Buffer (HB): 1:50 CHX + 1:100 DTT + 1:50…

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  • Suchergebnis: PAT_protocol_a290.pdf
    PAT_protocol_a290.pdf PDF, 103.77 KB Inhaltstyp: Dokument

    PAT Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in well, dice with…

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  • Suchergebnis: poly_A__tail_assay_a290.pdf
    poly_A__tail_assay_a290.pdf PDF, 101.16 KB Inhaltstyp: Dokument

    Poly(A) tail assay Homogenization 1. 2. 3. 4. 5. 6. 7. Prepare Homogenization Buffer with additives (HB+++) Add 1ml/well on 6‐well plate Add PFS fresh before use Place extracted spinal cord in…

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  • Suchergebnis: RNA_IP_Tissue_protocol_a290.pdf
    RNA_IP_Tissue_protocol_a290.pdf PDF, 105.78 KB Inhaltstyp: Dokument

    RNA IP Tissue Protocol IP RNA  Preparation of the beads        50µL Magnetic Prot A beads per condition (Dynabeads® Protein A, Invitrogen) Wash 2 x with PBS‐DEPC Block with yeast tRNA 50µg…

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