Single-cell Open Lab

RNAscope HiPlex Assay

We offer test chemistry, guidance and experimental support for HiPlex Assays. Both fresh frozen und FFPE samples can be analysed with pre-defined targeted FISH probes and single-cell resolution. Staining and imaging equipment is available via our booking system. 

10x Genomics Visium slide

While RNAscope provides single-cell resolution for up to 48 different pre-defined targets, visium sildes cover the whole transcriptome using spotted barcodes with a diameter 55 µm or with 2x2 µm square in case of visium HD. All steps including optimization, imaging, Cytassist transfer and library preparation can be done in our spatial lab. 

Molecular cartograhy

Molecular Cartography is a multiplex spatial analysis that allows the detection of up to 100 transcripts in intact tissue. Further readouts that complement the cyclic FISH signal can be recorded, such as H&E or protein IF.

Merfish

Merfish enables users to detect up to 500 transcripts in fresh frozen and FFPE samples. Subsequent antibody readouts can be performed for five protein targets using prelabeled secondary antibodies.

Xenium

The Xenium chemistry can detect up to 400 transcripts in fresh frozen and FFPE samples. Signal amplification via a rolling circle reaction allows the analysis of isoforms or fusions. Also 5k panels can be run on our system.

Each methodology has its merits (apart from the number of transcripts). If you are lost in space, we are happy to provide guidance in selecting the best technology for your project. Please discuss your spatial projects at an early stage with us - please do not order anything prior to a project discussion!

COMET by Lunaphore

We offer training and test antibodies to familiarize users with the seqIF workflow. Both fresh frozen und FFPE samples can be analysed. Staining and imaging is performed automatically by the system after successful panel optimization. 

Mibiscope by Ionpath

The MIBIscope allows the detection of over 40 markers simultaneously using single step imaging. The antibody based approach uses secondary ion mass spectrometry and thus requires labelling with elemental mass tags. To detect the tags the systems releases the secondary ions via a primary charged ion beam.

Each methodology has its merits. If you are lost in space, we are happy to provide guidance in selecting the best technology for your project. Please discuss your spatial projects at an early stage with us - please do not order anything prior to a project discussion!