Cookie Settings

We use cookies to optimize our website. These include cookies that are necessary for the operation of the site, as well as those that are only used for anonymous statistic. You can decide for yourself which categories you want to allow. Further information can be found in our data privacy protection .

Essential

These cookies are necessary to run the core functionalities of this website and cannot be disabled.

Name Webedition CMS
Purpose This cookie is required by the CMS (Content Management System) Webedition for the system to function correctly. Typically, this cookie is deleted when the browser is closed.
Name econda
Purpose Session cookie emos_jcsid for the web analysis software econda. This runs in the “anonymized measurement” mode. There is no personal reference. As soon as the user leaves the site, tracking is ended and all data in the browser are automatically deleted.
Statistics

These cookies help us understand how visitors interact with our website by collecting and analyzing information anonymously. Depending on the tool, one or more cookies are set by the provider.

Name econda
Purpose Statistics
External media

Content from external media platforms is blocked by default. If cookies from external media are accepted, access to this content no longer requires manual consent.

Name YouTube
Purpose Show YouTube content
Name Twitter
Purpose activate Twitter Feeds

Understanding the "wiring diagrams" of genes in complex tissues

No. 55 | 24/10/2018 | by Koh

How is the activity of all genes in cells of higher organisms interconnected? And how are the genetic "wiring diagrams" of the cells in complex tissues coordinated with each other? Scientists from the German Cancer Research Center, the European Molecular Biology Laboratory and the University of Heidelberg are now planning to investigate this in two model organisms, Drosophila and Arabidopsis. The European Research Council (ERC) is funding the "DECODE" project with a Synergy Grant.

© Benedikt Rauscher/DKFZ

Genes are team players. Changes in a genetic make-up often have an effect on the activity of a large number of other genes - with far-reaching consequences: "The interplay of very many gene activities determines the identity of a cell and ultimately decides whether, for example, a nerve cell or a white blood cell is formed - with largely identical genetic material," explains Michael Boutros from the German Cancer Research Center (DKFZ).

Wiring diagrams, which map the entirety of such genetic dependencies, were initially created for protozoa such as yeast and later also for cells of higher organisms in the culture dish. The challenge now is to extend such analyses to the complicated tissues of multicellular organisms, which usually consist of different cell types, differentiate in the course of life and have to react to changing environmental conditions.

With "DECODE", researchers from three Heidelberg institutions now want to prove that such analyses are possible. The European Research Council (ERC) is funding this ambitious project with a "Synergy Grant". In addition to Boutros, Wolfgang Huber from the European Molecular Biology Laboratory (EMBL), Jan Lohmann from the University of Heidelberg and Oliver Stegle from the DKFZ and EMBL are involved. The strength of the DECODE team is that it combines excellent expertise in single cell analysis and genome engineering with top-class bioinformatics.

The team will initially focus on two model systems: the intestine of the fruit fly Drosophila and the root tip of arabidopsis - the botanists' most popular research object. Using the CRISPR-Cas gene scissors, the researchers hope to switch off around 3000 selected genes, either individually or in pairs - and observe what happens: "Today we are able to analyze the RNA molecules at the level of individual cells. This enables us to recognize which changes in the activity of all genes our respective intervention has triggered," explains Michael Boutros.

The researchers hope to understand how the genetic circuit diagrams change in the course of the development and differentiation of a tissue or how they react to an external stimulus such as a toxin. "Drosophila and Arabidopsis have already been studied in detail and have compact genomes, making them particularly suitable for us. Our project is also paving the way for comparable studies on human cells," said Oliver Stegle. "If we understand disorders in the genetic circuit diagram of cells that occur in inflammations or cancer, for example, this could open up completely new possibilities for the development of new therapeutics.

With its "Synergy Grants", the ERC supports small teams of scientists working together to solve complex research problems, bringing together different techniques and skills across disciplines. The DECODE team won a highly competitive bidding process, with only one out of ten project proposals selected. "DECODE" will now receive €10.6 million over six years.

Video about the SYNGENE Grant

With more than 3,000 employees, the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) is Germany’s largest biomedical research institute. DKFZ scientists identify cancer risk factors, investigate how cancer progresses and develop new cancer prevention strategies. They are also developing new methods to diagnose tumors more precisely and treat cancer patients more successfully. The DKFZ's Cancer Information Service (KID) provides patients, interested citizens and experts with individual answers to questions relating to cancer.

To transfer promising approaches from cancer research to the clinic and thus improve the prognosis of cancer patients, the DKFZ cooperates with excellent research institutions and university hospitals throughout Germany:

  • National Center for Tumor Diseases (NCT, 6 sites)
  • German Cancer Consortium (DKTK, 8 sites)
  • Hopp Children's Cancer Center (KiTZ) Heidelberg
  • Helmholtz Institute for Translational Oncology (HI-TRON Mainz) - A Helmholtz Institute of the DKFZ
  • DKFZ-Hector Cancer Institute at the University Medical Center Mannheim
  • National Cancer Prevention Center (jointly with German Cancer Aid)
The DKFZ is 90 percent financed by the Federal Ministry of Education and Research and 10 percent by the state of Baden-Württemberg. The DKFZ is a member of the Helmholtz Association of German Research Centers.

RSS-Feed

Subscribe to our RSS-Feed.

to top
powered by webEdition CMS