Dr. Lena V. v. Voithenberg

Molecular and cellular heterogeneity in health and disease

We are interested in analyzing heterogeneity at the molecular and cellular level. Our main interest is the investigation of inter- and intrapatient heterogeneity of tumors and their microenvironment. To this end, we are using next-generation sequencing techniques and bioinformatic analyses to understand the role of differences at the genomic and transcriptomic level and in the cellular composition of the tumor samples.

Curriculum vitae

Lena obtained her PhD at the Ludwig-Maximilians-Universität München, investigating subpopulations of molecules by using single molecule fluorescence techniques. During a short research stay at the European Commission – Joint Research Centre, Lena extended her research towards open questions in oncology. While working in the Precision Diagnostics lab at IBM Research in Zurich, she developed techniques for the spatial analysis of tumor tissue sections. Currently, Lena is working as a team leader in the field of Applied Bioinformatics at the German Cancer Research Center.

Teaching

• RNA-sequencing as part of Bioinformatics Lecture
• Seminar Spatial Omics Techniques

Publications

2021

• Voith von Voithenberg L*, Kashyap A*, Opitz L, Aquino C, Sykes T, Nieser M, Petrini LFT, Enrriquez Casimiro N, van Kooten XF, Biskup S, Schlapbach R, Schraml P, Kaigala GV (2021) Mapping spatial genetic landscapes in tissue sections through microscale integration of sampling methodology into genomic workflows. Small. doi: 10.1002/smll.202007901.
• Voith von Voithenberg L, Barth A, Trauschke V, Demarco B, Tyagi S, Koehler C, Lemke EA, Lamb DC (2021) Comparative analysis of the coordinated motion of Hsp70s from different organelles observed by single molecular three-color FRET. PNAS. 118(33): e2025578118. doi: 10.1073/pnas.2025578118.

2019

• Voith von Voithenberg L, Fomitcheva Khartchenko A, Huber D, Schraml P, Kaigala GV (2019) Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity. Nucl. Acids Res. 48(3): e17. doi: 10.1093/nar/gkz1151.
• Voith von Voithenberg L*, Park J*, Stübe R, Lux C, Lee Y, Philippar K (2019) A novel prokaryote-type ECF/ABC transporter module in chloroplast metal homeostasis. Front. Plant Sci. 10:1264. doi: 10.3389/fpls.2019.01264.
• van Kooten XF, Petrini LFT, Kashyap A, Voith von Voithenberg L, Bercovici M, Kaigala GV (2019) Spatially Resolved Genetic Analysis of Tissue Sections Enabled by Microscale Flow Confinement Retrieval and Isotachophoretic Purification. Angewandte Chemie 58(43): 15259. doi: 10.1002/anie.201907150.
• Barth A, Voith von Voithenberg L, Lamb DC (2019) Quantitative Single-Molecule Three-Color Förster Resonance Energy Transfer by Photon Distribution Analysis. J. Phys. Chem. B. doi: 10.1021/acs.jpcb.9b02967.
• Voith von Voithenberg LϮ, Crocetti E, Martos Jimenez C, Dimitrova N, Giusti F, Randi G, Rooney R, Dyba T, Bettio M, N. Carvalho R (2019) Cancer registries – guardians of breast cancer biomarker information. A systematic review. Int. J. of Biological Markers. doi: 10.1177/1724600819836097.
• Heiss G*, Ploetz E*, Voith von Voithenberg L*, Vishwanathan R, Glaser S, Schluesche P, Madhira S, Meisterernst M, Auble D, Lamb DC (2019) Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation. Nucl. Acids Res. doi: 10.1093/nar/gky1322.

2018

• Huber D, Voith von Voithenberg L, Kaigala GV (2018) Fluorescence in situ hybridization (FISH): History, limitations and what to expect from micro-scale FISH? Micro and Nano Engineering 1:15.24. doi: 10.1016/j.mne.2018.10.006.
• Voith von Voithenberg LϮ, Lamb DC (2018) Single pair Förster resonance energy transfer: A versatile tool to investigate protein conformational dynamics. BioEssays. 40(3). doi: 10.1002/bies.201700078.

2017

• Sanchez-Rico C*, Voith von Voithenberg L*, Warner LR, Lamb DC, Sattler M (2017) Effects of fluorophore attachment on protein conformation and dynamics studied by spFRET and NMR. Chem Eur J. 23(57). doi: 10.1002/chem.201702423.

2016

• Voith von Voithenberg L, Sanchez-Rico C, Kang HS, Madl T, Zanier K, Barth A, Warner LR, Sattler M, Lamb DC (2016) Recognition of the 3'splice site RNA by the U2AF heterodimer involves a dynamic population shift. PNAS. 113(46):E7169-E7175. doi: 10.1073/pnas.1605873113.
• Lee DJ, Kessel E, Edinger D, He D, Klein PM, Voith von Voithenberg L, Lamb DC, Lächelt U, Lehto T, Wagner E (2016) Dual Antitumoral Potency of EG5 siRNA Nanoplexes Armed with Cytotoxic Bifunctional Glutamyl-Methotrexate Targeting Ligand. Biomaterials. 77:98-110. doi: 10.1016/j.biomaterials.2015.11.004.

2015 and earlier

• Barth A*, Voith von Voithenberg L*, Lamb DC (2015) MFD-PIE and PIE-FI: Ways to extract more information with TCSPC. Book Chapter: Springer Series on Fluorescence. 15:129-158. doi: 10.1007/4243_2014_66.
• Gietl A, Holzmeister P, Blombach F, Schulz S, Voith von Voithenberg L, Lamb DC, Werner F, Tinnefeld P, Grohmann D (2014). Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways. Nucleic Acids Res. 42(10):6219-31. doi: 10.1093/nar/gku273.
• Sikor M, Mapa K, Voith von Voithenberg L, Mokranjac D, Lamb DC (2013) Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET. EMBO J. 32(11):1639-49. doi: 10.1038/emboj.2013.89.
• Zimmermann D, Abdel Motaal B, Voith von Voithenberg L, Schliwa M, Ökten Z (2011). Diffusion of myosin V on microtubules: a fine-tuned interaction for which E-hooks are dispensable. PLoS One. 2011; 6(9):e25473. doi: 10.1371/journal.pone.0025473.

Patents

• Vogel R, Dogra N, Voith von Voithenberg L, Kashyap A, Kaigala G, Stolovitzky G. Determining position and transcriptomes of biological cells. US20200157528A1. Patent filed in 2018.