Functional Genome Analysis  (B070)
Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580
D-69120 Heidelberg, Germany.

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   Mapping and Sequencing of Xyllela fastidiosa
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figure of Xyllela genome map
Physical map of the X. fastidiosa genome.

Hybridisation probes are represented by horizontal lines, clones by vertical lines. A positive hybridisation signal is represented by a black spot at the cross section. The entire data set is presented, including all false positive or negative results. Clone contigs are numbered and gaps are indicated. Scattered lines indicate positions where a contig was thought to be continuous from hybridisation data while a small gap was identified by sequencing. On the right, the cosmids representing the megaplasmid are shown. R1 and R2 mark large repeat regions. At the bottom, the results obtained from hybridising genomic restriction fragments are shown; they were not taken into account for the actual clone ordering process and, thus, served as an independent control for co-linearity of the cosmid order.

Correlation of mapping and sequencing data

Comparison of clone locations as determined by mapping and end-sequencing information. Minor disturbances are mainly caused by the difference in resolution of mapping and sequencing. The few major inconsistencies were based on inaccurate mapping because of repeat sequences.

Mapping algorithm used for the
initial clone ordering.
Application of bootstrap techniques. Subsequently, the mapping was re-done using a different mapping algorithm. But for minor differences, the new algorithm produced an even better clone order. In addition, the comparison of mapping data generated with two independent methods proved helpful with respect to establishment of the final, correct clone order.



 
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Information on the entire genome sequence:
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http://aeg.lbi.ic.unicamp.br/xf/         Brazilian flag.
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