General strategy

  • Generation of a (expression-competent) DNA-construct, CRISPR/Cas reagents or genetically modified ES cells
  • Isolation of early embryos (zygotes or blastocysts)
  • Injection of DNA, CRISPR/Cas reagents or genetically modified ES cells and transfer of the injected embryos into a foster mouse
  • Analysis of the offspring, to check for transgenesis, by testing tail-DNA, ear biopsies or blood
  • Analysis of the expression and biological effects of the gene of interest in the transgenic animals

Methods

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