Generation of transgenic mice

PhD Program: 0,5 Credit Points

The Transgenic Service organizes a yearly course "Generation of transgenic (genetically altered) mice".

This Course is for interested colleagues at the DKFZ.

Registration: DKFZ Training Portal (Advanced training and In-service training M300)

Next courses:

Course in German
Part I – Theory     Tuesday, January 26th 2021 09:30 - 11:00 hrs
Part II – Practice  Cancelled in 2021

Course in English
Part I – Theory     Tuesday, February 2nd  2021  09:30 - 11:00 hrs
Part II – Practice  Cancelled in 2021

Theory: online
Practice: Cancelled in 2021

Part I – Theory

· What are transgenic mice?
· Usage of transgenic mice
· Injection of DNA into pronucleus of zygotes
· Embryonic stem cells and homologous recombination
· Cre/loxP system
· Injection of embryonic stem cells into blastocysts
· Alternative methods
· Cryopreservation of embryos and sperm from mice
· Vasectomy of male mice
Part II – Practice (demonstration)  Cancelled in 2021

· Introduction into isolator housing system
· Collection of mouse embryos (zygotes and morulae)
· Injection of embryonic stem cells into blastocysts and morulae
· Injection of DNA into pronucleus and cytosol of zygotes
· Transfer of embryos into the uterus
Participation of part I is necessary for visiting the practical part.

to top
powered by webEdition CMS