Generation of transgenic mice

PhD Program: 0,5 Credit Points

The Transgenic Service organizes a yearly course "Generation of transgenic (genetically altered) mice".

This Course is for interested colleagues at the DKFZ.

Registration: At HCM portal (Training and In-service training M300)

Next courses:

Course in German
Part I – Theory    January 19th  2017 9:00 – 12:00 hrs
Part II – Practice January 26st  2017 8:30 – 13:00 hrs

Course in English
Part I – Theory     January 24th  2017  9:00 – 12:00 hrs
Part II – Practice February 02nd  2017 8:30 – 13:00 hrs

Theory: 8th floor, room-No. 824
Practice: Transgenic Service at the DKFZ, 1st floor, main building

Part I – Theory

· Transgenic animal models - for what reason?
· General strategy for generation of transgenic mice
· Injection of DNA into pronucleus of zygotes
· Embryonic stem cells and homologous recombination
· Cre/loxP system
· Injection of embryonic stem cells into blastocysts
· Alternative methods
· Cryopreservation of embryos and sperm from mice
· Vasectomy of male mice
Part II – Practice (demonstration)

· Introduction into mice housing
· Collection of mouse embryos (zygotes and morulae)
· Injection of embryonic stem cells into blastocysts
· Injection of DNA into pronucleus of zygotes
· Transfer of embryos into the oviduct and uterus
Participation of part I is necessary for visiting the practical part.

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