WEBVTT
Kind: subtitles
Language: en

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Hi, I'm Emily and I'm currently doing my Master's 
Thesis at the German Cancer Research Center.

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At the moment I'm doing a lot of cell 
culture because my project includes biology  

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and I'm working on combination therapies so I 
try to combine the irradiation with different  

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concentrations of DNA repair inhibitors 
to create a synergistic effect of both  

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and for that I'm doing so-called 
clonogenic assays. I plate cells and  

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I can fix and stain them in the end and 
then I can count the surviving colonies  

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and for that I can determine the survival 
fraction in the end and see how many cells  

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survive with the different concentrations 
and in combination with the irradiation.  

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These are some examples of my plates and this one 
here for example is from a pancreatic cancer cell  

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line where I tried to irradiate at two Gray and I 
used a first concentration which was higher than  

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the second one and here in the first concentration 
there are lots more of colonies. It means the  

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second concentration worked in combination with 
the irradiation. Additionally, I will do this in  

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3D because tumors are also growing in 3D so I'm 
going to create so-called spheroids and I will  

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observe the increase and the shrinkage of their 
size and this is called a growth delay essay.  

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I like most about my project that it includes a 
lot of practical work. I really have to plan my  

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own experiments. I have to look after the cells 
and I have to look if my experiments work or not  

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and in the end I have to do my own analysis. And 
I really like that in medical physics a lot of  

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different fields work together. I really get 
to know how to apply physics and biology here.  

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I have a lot of colleagues who are biologists 
chemists physicists and medical students as well.  

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My daily challenges include the work with the 
cells because if you slightly change something  

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in the environment of the experiment, it can 
happen that the cells behave totally different so  

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you have to be very accurate at working with the 
experiment. And the biological experiments, they  

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take a lot of time so in the end if it didn't work 
you have to repeat the whole experiment again.

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you