Practical Course HP-ZMBH: Yeast: Basic Knowledge and Methods

Type: Practical Course with Student Seminars

Date: 28. January - 15. February 2019

Hosts/Supervisors: Elmar Schiebel, Gislene Pereira

Synopsis:

Note added for WS 2018/2019:
For organizational reasons the course has to be slightly re-organized, the participants will be informed directly.

The intention of this master practical is to learn basic methods that are relevant for working successfully with the model system budding yeast Saccharomyces cerevisiae. Participants will learn the ‘dos and donts’ when working with yeast, where to obtain information and resources, methodological workflows, experimental planning etc. At the end of the course students will possess all relevant knowledge to start working with yeast, either in the setting of a ‘yeast lab’, or in the environment of a lab that exploits the advantages of yeast for basic and applied research and in biotechnology.

The course consists of 3 modules that are coherent entities, each prepared by a different lab. Together, the 3 modules cover all relevant aspects to get you ‘started’ with yeast. To participate in Modules 2 or 3 it is mandatory to participate in the previous modules.

Topics and Content

Module 1: Theory and basics lab knowledge (Prof. Michael Knop, ZMBH)

This module consists of a lecture series that introduces yeast as a model organism and covers many practical and theoretical aspects. The hands-on lessons will teach the very basic lab manipulations with yeast with a focus on ‘good microbiological practice’.

  • 5 theory lectures à 90 min
  • 5 hands-on lessons à 4 hours

Module 2: Seminal findings made with yeast (Prof. Elmar Schiebel, ZMBH)

This module consists of student presentations of key scientific papers of the last 150 years where yeast played a major role in many scientific discoveries, and it encompasses hands on experimental work on basic experimental techniques: genetics and molecular biology methods and strain constructions. We will use yeast in an evolution experiment to access how an organism can bypass a clinically relevant drug.

  • 10 presentations à 45 min by the participants
  • 5 hands-on lessons à 4 hours

Module 3: Yeast functional analysis: from cell biology to genomics (Prof. Gislene Pereira, COS)

During this module several advanced experimental techniques will be discussed and different typical experimental work-flows will be demonstrated.

  • 4 hands-on lessons à 6 hours
  • 1 wrap up session

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