Practical Course HP-F3: Epigenetics & Epitranscriptomics, Crystallography, and Transplantation Models to Study Brain Cancer and Stem Cell Biology

Type: Practical Course with Student Seminars

 

Part 1: Transplantation models to study brain cancer and stem cell biology

Date: 23.-27. November 2020

Location: DKFZ Teaching lab, A231 Division Labs (DKFZ Main Building, 2nd floor) and COS Division Labs (INF 230, Rooms 002, 006 and 05.011)

Hosts/Supervisors: Julieta Alfonso (contact: j.alfonso@dkfz.de), Ceren Duman, Lazaro Centanin

 

Topics:

Cell transplantations in mice and fish.

Experimental setups and data analysis to study brain tumor and stem cell biology.

 

Content:

  • Experimental design to test the role of a protein of interest in glioblastoma growth
  • Genetic labeling of glioblastoma cells in culture
  • Tissue processing and analysis of tumor samples from experimental animals
  • Genetic and chemical labeling of embryonic stem cells in fish
  • Embryonic Stem Cell transplantations in fish

Part 2: Crystallography

© dkfz.de

Date: 30. November - 4. December 2020

Location: DKFZ Teaching Lab and D160 Division Labs (DKFZ Main Building, 7th floor)

Hosts/Supervisors: Erec Stebbins, Johan Zeelen, Alex Hempelmann, and Monica Chandra (contact: e.stebbins@dkfz.de)

 

Topic:

Crystallography: A tool to understand protein-protein interactions and drug discovery 

 

Content:

  • Practical introduction to crystallography
  • Crystallography and cancer research
  • Crystallization of a protein
  • Solving the structure of a protein

Part 3: Epigenetics and epitranscriptomics: DNA and RNA modifications and their effect on gene expression

Date: 7.-11. December 2020

Location: DKFZ Teaching Lab and D150 Division Labs (DKFZ Main Building, 7th floor)

Hosts/Supervisors: Nina Papavasiliou, Riccardo Pecori and co-workers (contact: n.papavasiliou@dkfz.de)

 

Topic:

Epigenetics and epitranscriptomics: DNA and RNA modifications and their effect on gene expression

 

Content:

Nucleic acid modifications: from chemical detection to biological “sensing” and downstream outcomes (including gene expression changes and DNA repair).

Functional assays to assess modifications (including tissue culture and FACS approaches; also RT-PCR of target RNA, cloning and sanger sequencing for the identification of DNA or RNA modifications).

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