Practical Course HP-F12: Immunological Methods

Type: Practical Course with Student Seminars

Date: 15. June - 3. July 2020

 

Hosts/Supervisors:

Christian Busse, Stefan Eichmüller, Viktor Umansky (responsible organizer contacts: christian.busse@dkfz.de, s.eichmueller@dkfz.de, v.umansky@dkfz.de)
Guoliang Cui, Branko Cirovic, Nikolaus Dietlein, Christine Engeland, Thorsten Feyerabend, Johannes Heidbüchel, Katharina Imkeller Wolfram Osen, Thomas Plum, Sandrine Sander

Topics:

Methods in experimental immunology and tumor immunology including cellular analysis, single-cell assays, sequencing, and various functional assays. The practical course covers a selection of these methods routinely applied by research groups working in the tumor immunology field.

Contents (practical part):

  • Introduction into flow cytometry and cell sorting
  • B cell development
  • Sequence and repertoire analysis of immunoglobulins
  • Affinity maturation of antibodies
  • Quantification of the type 1 hyper-reactive mast cell degranulation
  • T cell metabolism
  • Bi-specific T-cell engagers (BiTEs)
  • Phagocytosis of apoptotic cells
  • Detection of immune cell infiltration in tumors using immunofluorescence techniques
  • Detection of antigen-specific T cells using FACS-based cytokine secretion assay
  • Functional T cell assays: ICS, ELISPOT, ELISA

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