Cookie Settings

We use cookies to optimize our website. These include cookies that are necessary for the operation of the site, as well as those that are only used for anonymous statistic. You can decide for yourself which categories you want to allow. Further information can be found in our data privacy protection .


These cookies are necessary to run the core functionalities of this website and cannot be disabled.

Name Webedition CMS
Purpose This cookie is required by the CMS (Content Management System) Webedition for the system to function correctly. Typically, this cookie is deleted when the browser is closed.
Name econda
Purpose Session cookie emos_jcsid for the web analysis software econda. This runs in the “anonymized measurement” mode. There is no personal reference. As soon as the user leaves the site, tracking is ended and all data in the browser are automatically deleted.

These cookies help us understand how visitors interact with our website by collecting and analyzing information anonymously. Depending on the tool, one or more cookies are set by the provider.

Name econda
Purpose Statistics
External media

Content from external media platforms is blocked by default. If cookies from external media are accepted, access to this content no longer requires manual consent.

Name YouTube
Purpose Show YouTube content
Name Twitter
Purpose activate Twitter Feeds

Vector Production & Development Unit (VP&DU)


Group 2018 more

Group in 2017
© 2018 Tom Holz (

The VP&D Unit supports research-associated groups on two levels:

  • Large Scale production of purified virus (vector) stocks for preclinical applications or basic research
  • Development and optimization of new production, purification and analytic methods with the aim to establish working protocols for GMP-production of virus(vector) stocks necessary for clinical applications planned in the department F010.


  • High virus yield and concentration
  • High purity
  • Precise analysis (quantity and quality)
  • Good process control
  • High safety
  • Process development including upscaling


  • Standard large-scale production of purified, well characterized virus stocks of wildtype and recombinant parvoviruses.
  • Accompanying research for clinical studies.
  • Consulting and technical support intern by questions to virus production, purification and analysis.
  • Optimization and adaptation of production and purification methods.

Beside providing support for scientists our aim is to establish standard protocols for:
(a) self-replicating wild type and mutant parvoviruses and
(b) recombinant autonomous and adeno-associated parvoviruses carrying foreign therapeutic genes.

These new developments concern:

  • the improvement of distinct steps in the production and purification protocols (e.g. evaluation of initial titers for virus production [optimization of per cell output], optimizing transfection procedures [cost factors included], search for suitable cell lines for optimal production), 
  • up-scaling of the currently used procedures (evaluation of suitable production factories, adaptation to suspension/micro-carrier cultures), 
  • the establishment of new procedures for virus purification and quality controls (concentration of virus stocks, purification by alternative/additional purification steps [e.g. affinity chromatography], establishment of quality control measurements, (SDS-PAGE, HPLC/FPLC, Empty capsid vs. full capsids by ELISA-techniques), 
  • adaptation of present materials and methodologies to the production of GMP-grade virus (vector) stocks to support clinical trials

In the establishment of GMP-produced parvovirus, the unit also plays a pivotal role in the interaction of the F010 division with the various organizations involved in the approval and application of procedures for virus (vector) production under GMP-conditions.

to top
powered by webEdition CMS