Supplementary Table 1: JAK/STAT phenotypes by RNAi [pdf]

 
Gene name dsRNA ID z-score
[screen]
z-score
[re-screen]
Functional group
assignment (based on GO
and Interpro evidence)
Interpro 8.0
evidence
GO Evidence
 

Positive Regulators

 
Art2 HFA00627 -2,9 -3,2 Protein modifying enzymes
Metabolism
IPR000051 GO:0016274
protein-arginine N-methyltransferase activity
asf1 HFA11324 -2,3 -2,5 Others IPR008967 GO:0003682
chromatin binding
bin3 HFA04919 -3,1 -3,3 Unknown IPR000051 na
na
CG10007 HFA14173 -3,2 -2,9 Unknown noIPR na
na
CG10730 HFA02102 -2,1 -2,3 Unknown IPR004245 na
na
CG10960 HFA09807 -2,0 -2,1 Protein modifying enzymes
Metabolism
IPR005829 GO:0005355
glucose transporter activity
CG11307 HFA11648 -2,3 -2,4 Unknown noIPR GO:0016757
transferase activity
CG11696 HFA19417 -2,0 -2,3 Transcription regulators IPR007087 GO:0003677
DNA binding
CG12213 HFA14478 -3,3 -3,2 Unknown IPR009053 na
na
CG12460 HFA20970 -3,3 -3,4 Transcription regulators IPR000504 GO:0030528
transcription regulator activity
CG12479 HFA19459 -2,3 -2,4 Unknown IPR007512 na
na
CG13243 HFA01920 -2,7 -2,6 Unknown IPR003117 na
na
CG13473 HFA10017 -2,4 -2,1 Cytoskeleton and Transport IPR006662 GO:0005489
electron transporter activity
CG14434 HFA17927 -2,0 -2,3 Unknown IPR008173 na
na
CG15306 HFA17993 -3,3 -3,1 Signal transduction IPR001715 GO:0005102
receptor binding
CG15418 HFA00432 -2,1 -2,1 Protein modifying enzymes
Metabolism
IPR002223 GO:0004866
endopeptidase inhibitor activity
CG15434 HFA00449 -2,5 -2,9 Protein modifying enzymes
Metabolism
IPR007741 GO:0003954
NADH dehydrogenase activity
CG15555 HFA15093 -2,3 -2,6 Others IPR001873 GO:0015268
alpha-type channel activity
CG15784 HFA18090 -2,4 -2,6 Unknown IPR009072 na
na
CG16903 HFA18561 -2,8 -2,8 Transcription regulators IPR011028 GO:0016251
general RNA polymerase II transcription factor activity
CG17179 HFA10258 -2,1 -2,8 Unknown IPR001660 na
na
CG18160 HFA21006 -3,1 -2,4 Unknown noIPR na
na
CG30069 HFA06272 -2,9 -2,2 Protein modifying enzymes
Metabolism
noIPR GO:0016491
oxidoreductase activity
CG3058 HFA00563 -3,4 -3,5 Cytoskeleton and Transport IPR006663 GO:0005489
electron transporter activity
CG31005 HFA15507 -2,3 -3,0 Protein modifying enzymes
Metabolism
IPR000092 GO:0000010
trans-
hexaprenyltranstransferase
activity
CG31132 HFA16032 -2,8 -3,5 Unknown IPR001487 na
na
CG31132 HFA15369 -2,3 -3,6 Unknown IPR001487 na
na
CG31358 HFA15235 -2,0 -2,2 Cytoskeleton and Transport IPR001972 GO:0005200
structural constituent of cytoskeleton
CG31694 HFA00415 -2,8 -2,7 Signal transduction IPR006921 GO:0005102
receptor binding
CG32406 HFA09966 -2,1 -2,2 Signal transduction IPR000980 na
na
CG32573 HFA19906 -3,1 -2,9 Unknown IPR000719 na
na
CG3281 HFA15470 -3,1 -3,0 Transcription regulators IPR007087 GO:0030528
transcription regulator activity
CG3819 HFA10378 -2,3 -2,3 Unknown IPR001604 na
na
CG4022 HFA10395 -3,4 -3,7 Unknown noIPR na
na
CG40351 HFA20930 -2,6 -2,7 Transcription regulators IPR001214 GO:0030528
transcription regulator activity
CG4349 HFA19892 -4,1 -2,1 Others IPR009040 GO:0008199
ferric iron binding
CG4446 HFA10420 -2,7 -2,7 Protein modifying enzymes
Metabolism
IPR004625 GO:0008478
pyridoxal kinase activity
CG4653 HFA19909 -3,2 -3,0 Protein modifying enzymes
Metabolism
IPR001254 GO:0004263
chymotrypsin activity
CG4781 HFA04488 -2,5 -2,5 Unknown IPR003591 na
na
CG6422 HFA16036 -3,3 -3,2 Unknown IPR007275 na
na
CG6434 HFA10635 -2,8 -2,8 Unknown IPR001680 na
na
CG6946 HFA16145 -2,3 -2,9 RNA processing and Translation IPR000504 GO:0003723
RNA binding
CG7635 HFA20054 -2,9 -2,8 Cytoskeleton and Transport IPR001972 GO:0005200
structural constituent of cytoskeleton
CG8108 HFA09675 -2,7 -2,7 Transcription regulators IPR007087 GO:0003676
nucleic acid binding
CG9086 HFA20148 -2,8 -2,9 Signal transduction IPR009030 GO:0005057
receptor signaling protein activity
CkIIalpha HFA11946 -2,1 -2,5 Signal transduction IPR000719 GO:0004702
receptor signaling protein serine/threonine kinase activity
CkIIbeta HFA20230 -2,7 -2,6 Signal transduction IPR000704 GO:0004702
receptor signaling protein serine/threonine kinase activity
comm3 HFA09995 -2,2 -2,2 Unknown noIPR na
na
CtBP HFA16617 -2,9 -2,8 Transcription regulators IPR006139 GO:0003714
transcription corepressor activity
dome HFA19583 -6,2 -4,9 Signal transduction IPR000194 GO:0004907
interleukin receptor activity
eIF-4B HFA20983 -3,2 -3,0 RNA processing and Translation IPR000504 GO:0003723
RNA binding
HDC01676 HFA01091 -2,3 -2,6 Unknown IPR006202 na
na
HDC11198 HFA11427 -2,3 -2,2 Unknown noIPR na
na
hop HFA20340 -5,7 -4,1 Signal transduction IPR001245 GO:0004718
Janus kinase activity
Ipk2 HFA00357 -2,6 -4,0 Signal transduction IPR005522 GO:0050516
inositol-polyphosphate multikinase activity
jbug HFA04167 -2,7 -3,2 Cytoskeleton and Transport IPR001298 GO:0005200
structural constituent of cytoskeleton
kn HFA07637 -2,4 -2,4 Transcription regulators IPR003523 GO:0030528
transcription regulator activity
l(1)G0084 HFA19450 -2,1 -2,1 Transcription regulators IPR001965 GO:0003677
DNA binding
larp HFA16984 -2,5 -2,4 Unknown IPR006630 na
na
mask HFA15370 -2,3 -2,7 Signal transduction IPR002110 GO:0005102
receptor binding
mst HFA20582 -2,2 -2,6 Unknown noIPR na
na
nonA HFA20357 -3,0 -3,3 RNA processing and Translation IPR000504 GO:0030528
transcription regulator activity
Obp93a HFA15220 -2,4 -2,9 Cytoskeleton and Transport IPR006170 GO:0005549
odorant binding
Rrp1 HFA00784 -4,3 -4,3 Others IPR000097 GO:0004520
endodeoxyribonuclease activity
sol HFA20587 -2,5 -3,0 Others IPR001876 GO:0005516
calmodulin binding
Stat92E HFA16870 -5,0 -5,2 Signal transduction IPR001217 GO:0004871
signal transducer activity
Taf2 HFA11298 -2,7 -2,9 Transcription regulators IPR002052 GO:0016251
general RNA polymerase II transcription factor activity
 

Negative Regulators

 
bon HFA16914 5,6 4,8 Protein modifying enzymes
Metabolism
IPR001841 GO:0004842
ubiquitin-protein ligase activity
Caf1 HFA16596 3,0 2,6 Protein modifying enzymes
Metabolism
IPR001680 GO:0035035
histone acetyltransferase binding
CG10077 HFA09691 2,8 4,0 RNA processing and Translation IPR001410 GO:0003724
RNA helicase activity
CG11400 HFA06070 2,6 2,2 Unknown noIPR na
na
CG11501 HFA14317 3,7 3,1 Unknown noIPR na
na
CG13499 HFA04144 2,5 3,1 Unknown noIPR na
na
CG14247 HFA14742 3,2 3,4 Unknown IPR002557 na
na
CG15706 HFA06577 2,2 2,1 Unknown IPR011701 na
na
CG16975 HFA02552 2,7 2,7 Transcription regulators IPR001660 GO:0030528
transcription regulator activity
CG17492 HFA02623 2,5 2,1 Protein modifying enzymes
Metabolism
IPR001841 GO:0004842
ubiquitin-protein ligase activity
CG18112 HFA15304 2,1 2,1 Unknown IPR001829 na
na
CG30122 HFA06935 3,3 2,8 Transcription regulators IPR003034 GO:0003677
DNA binding
CG4907 HFA15673 3,3 3,5 Unknown IPR007070 na
na
dre4 HFA08714 2,6 2,5 Transcription regulators IPR000994 GO:0003712
transcription cofactor activity
enok HFA04096 3,0 3,0 Transcription regulators IPR001965 GO:0030528
transcription regulator activity
lig HFA07247 2,2 2,1 Unknown IPR009060 na
na
Nup154 HFA03384 2,9 2,9 Cytoskeleton and Transport IPR011045 GO:0005487
nucleocytoplasmic transporter activity
par-1 HFA07660 4,4 4,2 Signal transduction IPR000719 GO:0004674
protein serine/threonine kinase activity
Pp1alpha-96A HFA16795 3,0 3,8 Signal transduction IPR006186 GO:0004722
protein serine/threonine phosphatase activity
PP2A-B' HFA16344 2,6 2,5 Signal transduction IPR002554 GO:0008601
protein phosphatase type 2A regulator activity
Ptp61F HFA08683 5,9 8,1 Signal transduction IPR000863 GO:0004725
protein tyrosine phosphatase activity
Rab5 HFA00777 2,1 2,1 Signal transduction IPR001806 GO:0005525
GTP binding
Socs36E HFA02455 3,2 2,3 Signal transduction IPR000980 GO:0007259
JAK-STAT cascade
TSG101 HFA11098 3,1 3,4 Protein modifying enzymes
Metabolism
IPR001440 GO:0004842
ubiquitin-protein ligase activity
 
InterPro Evidence was obtained from:
Mulder et al. (2005). InterPro, progress and status in 2005.

GO Evidence was obtained from:
R.A. Drysdale, M.A. Crosby and The FlyBase Consortium (2005). FlyBase: genes and gene models. Nucleic Acids Research 33:D390-D395. http://flybase.org/

All 384-well screening plates contained dsRNAs against known JAK/STAT pathway components.
Controls for the 57 screening plates were stat92E RNAi (identified 55 times), hop RNAi (identified 37 times), dome RNAi (identified 55 times) and socs36E RNAi (identified 45 times)
 
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