Performance and advantages

• sequence up to 16 independent samples in one run (up to 192 independent samples by using MID-tags)
• sequence ~400 Mb in less than ten hours
• read length 200-400 bp
• ~1 million high quality reads per run (depending on the application)
• single base read accuracy >99%
• consensus accuracy >99.99%

Applications for the 454/GS FLX technology

Whole genome sequencing (de novo, resequencing) »...

• Shotgun Sequencing (bacteria, viruses)
• BAC-based Shotgun Sequencing (animals, plants)

Transcriptome analysis »...

• EST sequencing
• Genome identification sequencing (GIS-PET)

Gene regulation studies »...

• Identification of protein binding sites
• Analysis of DNA methylation patterns

Amplicon sequencing »...

Sequence based karyotyping »...

• Identification of copy number variations
• Detection of SNPs, Inserts, Deletions

Metagenomics »...

Further details concerning the Roche/454 GS FLX Titanium sequencing can also be obtained from the Roche/454 web site and the list of puplications.

You provide

• genomic samples: 100 µl genomic DNA dissolved in TE  with a DNA concentration of at least 50 ng/µl (Nanodrop)
• Amplicons: 30 µl of your probe dissolved in TE with a DNA concentration of more than 25ng/µl (Nanodrop) (» Amplicon Primer Sequences)

You receive

• QC data and run statistics
• complete raw sequence (FASTA format) and quality data (additional files such as raw sff-files are also available)
• mapping, assembly and amplicon analysis data and statistics
• bioinformatics support for further analysis

For more information and experimental setup please contact us directly (Dr. Stephan Wolf).