Protein Analysis

The Protein Analysis Facility provides full-services in protein identification by mass spectometry and assists researchers to identify and characterize proteins and find differential protein expression. The core facility serves the research community by providing a resource for training, education, and consultation in protein identification and modern proteomic techniques.

Molecular Structure Analysis

The Molecular Structure Analysis Unit (MSA) offers the following services

• Analytical High-Field NMR Spectroscopy
• Mass Spectrometry
• Molecular Modeling & Glycomics Web Services

Protein Protein Interaction Analysis

Yeast Two Hybrid

Yeast Two-Hybrid (Y2H) screening is a well-established method to detect protein protein interactions.

Lumier

LUMIER-assays (LUminescence-based Mammalian IntERactome mapping), originally published by Barrios-Rodiles et al., provide a novel method to test protein-protein interactions. It is based on the co-purification of two tagged proteins from transiently transfected mammalian cells.

Protein Purification

Proteins play a crucial role in all life processes. They serve as construction materials, transmit messages from cell to cell, and are responsible for regulating metabolism. Proteins are long chains of amino acids. In cancer research, purified proteins are used for a wide variety of applications, including assay development, molecular biology applications, raise of antibodies, binding studies and more. Therefore, protein purification is a first step of almost all in vitro protein studies. High quality of purified protein could be critical in respect to their activity and folding. Proteins can be isolated from their native environment or from recombinant sources like E. coli, yeast, baculo virus or mammalian cell culture.

Peptide Synthesis

Proteins play a pivotal role in all life processes. They serve as construction materials, transmit messages from cell to cell, and are responsible for regulating metabolism. Proteins are long chains of amino acids, shorter chains are called peptides. Our working group produces peptides for different scientific purposes.