Protein Analysis

The Protein Analysis Facility provides full-services in protein identification by mass spectometry and assists researchers to identify and characterize proteins and find differential protein expression. The core facility serves the research community by providing a resource for training, education, and consultation in protein identification and modern proteomic techniques.

Molecular Structure Analysis

The Molecular Structure Analysis Unit (MSA) offers the following services

• Analytical High-Field NMR Spectroscopy
• Mass Spectrometry
• Molecular Modeling & Glycomics Web Services

Protein Protein Interaction Analysis

Yeast Two Hybrid

Yeast Two-Hybrid (Y2H)  is the method of choice for the initial detection of binary protein-protein interactions.
We have a large screening database which is helpful to assess beforehand the chances of screening the protein or protein fragment of interest.
We can offer to look at your protein in detail, suggest appropriate screening libraries and check for possible artifactual binding tendencies (promiscuity).

Pairwise Y2H tests can be used to verify individual protein interactions as well as for domain and epitope mapping and binding studies of mutated proteins/domains.

As a new service we offer the possibility to measure a broad range of biomolecular interactions including the determination of binding parameters.

Protein Purification

Proteins play a crucial role in all life processes. They serve as construction materials, transmit messages from cell to cell, and are responsible for regulating metabolism. Proteins are long chains of amino acids. In cancer research, purified proteins are used for a wide variety of applications, including assay development, molecular biology applications, raise of antibodies, binding studies and more. Therefore, protein purification is a first step of almost all in vitro protein studies. High quality of purified protein could be critical in respect to their activity and folding. Proteins can be isolated from their native environment or from recombinant sources like E. coli, yeast, baculo virus or mammalian cell culture.

Peptide Synthesis

Proteins play a pivotal role in all life processes. They serve as construction materials, transmit messages from cell to cell, and are responsible for regulating metabolism. Proteins are long chains of amino acids, shorter chains are called peptides. Our working group produces peptides for different scientific purposes.