Illumina HiSeq 2000

The Illumina HiSeq 2000 is a short read system that uses a massively parallel sequencing-by-synthesis approach to generate billions of bases of high-quality sequence data per run. With selectable read lengths of 50 bp or 100 bp and the option of paired-end sequencing, this technology can be used for a wide variety of applications. Furthermore, barcoding (multiplexing) increases the number of samples that can be sequenced in parallel.

Performance and advantages

• sequence up to 16 independent samples on 2 flowcells in one run
• 12 unique barcodes provided by Illumina for multiplexing
• ~ 800 million high-quality reads per flowcell (~100 million/lane)
• up to 600 Gb output per run
• >80% bases higher than Q30 (at 2x100 bp)
• single-read (1 x 50 bp; 1 x 100 bp) or paired-end sequencing (2 x 100bp)

Further details concerning the Illumina HiSeq 2000 sequencing can also be obtained from the Illumina web site and the list of puplications.

You provide

• genomic samples: 1 µg genomic DNA
• fragmented immunoprecipitated (IP) DNA (e.g. ChIP): 10 ng with fragment size ~100-200 bp
• mRNA: 500 ng
• total RNA: 5 µg

You may provide the samples together with the HiSeq 2000 submission form, after logging into the GPCF web (see left menu).

You receive

• QC data and run statistics
• complete raw sequence and quality data (Illumina FASTQ format)
• mapping data and statistics (Illumina EXPORT or SAM/BAM format)
• bioinformatics support for further analysis
• the data returned depend on the sequencing application (please contact the High Throughput Sequencing Support for further information)

Please be aware, the raw image output of a sequencing run is not stored by the HiSeq 2000 sequencer and therefore can not be provided.

For more information and experimental setup please contact us directly (Dr. Stephan Wolf).