As a response to investigator/cooperation partner feedback, the MAF offers a rapid immunization protocol allowing cell fusion at day 8 and the generation of mouse monoclonal cell lines in 4 to 5 weeks or, also available by request, cell fusion at day 15 and generation of rat monoclonal cell lines in 6 to 8 weeks.
Contaminations of cell culture pose a major threat to scientific results. In addition to molds, yeast and bacteria contaminations that are easily detectable by pH shifts, turbidity, and cell destruction, other contaminations such as viruses, mycoplasma and other cell lines are more difficult to detect, and as a result, are a potentially more serious problem.
Cell cultures are important tools for basic and applied biomedical research. However, their use typically involves several investigators and exchange between laboratories bearing the risk for contamination with cells of other origin. The need for cell line authentication has been emphasized by granting agencies as well as by many high ranking journals (no cell authentication = no grant/publication).