Introduction

Contaminations of cell culture pose a major threat to scientific results. In addition to molds, yeast and bacteria contaminations that are easily detectable by pH shifts, turbidity, and cell destruction, other contaminations such as viruses, mycoplasma and other cell lines are more difficult to detect, and as a result, are a potentially more serious problem.

The DKFZ has developed a high-throughput multiplex cell contamination test (McCT) currently able to detect >20 contamination markers in a single reaction.

Multiplex cell Contamination Testing (McCT) v.2 Service

Contaminations of cell culture pose a major threat to scientific results and their interpretation. In addition to molds, yeast and bacteria contaminations that are easily detectable by pH shifts, turbidity, and cell destruction, other contaminations such as viruses, mycoplasma and other cell lines are more difficult to detect, and as a result, are a potentially more serious problem. The DKFZ has developed a high-throughput multiplex cell contamination test that is currently able to detect >20 contamination markers in a single reaction.

This and similar assays are meanwhile carried out by a number of different commercial providers. Since the unit that originally developed this assay at DKFZ has meanwhile been outsorced please go to the internal pages to obtain further information on available providers and services.