Protein Analytics Instrumentation
The Protein Analysis Facility provides services and assists researchers to identify and characterize proteins and differential protein expression. The core facility serves the research community by providing a resource for training, education, and consultation in protein identification and modern proteomic techniques.
The equipment can be divided in four categories:
equipment for protein separation and imaging,
sample preparation for mass spectrometry,
mass spectrometers and
computer equipment with the appropriate software for data evaluation and database searches.
- Protein Separation and Imaging:
2D gel equipment:
Isoelectric focussing cell (ETTAN IPGphor, Amersham Bioscience)
2D gel chamber (DALTtwelve System, Amersham Bioscience)
High resolution gel imaging system (Image Scanner, Amersham Bioscience)
2-D Fluorescence difference gel electrophoresis (2D DIGE, Amersham Bioscience) - Sample Preparation:
2 benches for gel slicing and digestion to prevent sample contamination
Manual gel spotter (Gelpal, Genetix) - Mass Spectrometry:
MALDI time of Flight PSD reflectron and linear mass spectrometer (Reflex II, Bruker)
MALDI Time of Flight reflectron mass spectrometer (M@LDI, Waters)
Quadrupole Time of Flight electrospray tandem mass spectrometer (Q-Tof Ultima, Waters) with a nano flow HPLC system (CapLC System, Waters)
Ion trap mass spectrometer (LCQ, Thermo Electron ) with a nano flow HPLC system (UltiMate, Dionex) - Computer and Software Equipment:
Proteomweaver (Definiens) 2D image analysis software
Mascot protein identification database on a 4 processor Linux cluster
Profoud protein identification database on a 2 processor PC
Protein Global Lynx mass spectrometry processing and identification software (Waters) for protein identification, automated BLAST searches, automated DeNovo sequencing analysis and quantitative proteomics.
last update:
06/06/2011
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